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3.2.1.55: non-reducing end alpha-L-arabinofuranosidase

This is an abbreviated version!
For detailed information about non-reducing end alpha-L-arabinofuranosidase, go to the full flat file.

Word Map on EC 3.2.1.55

Reaction

1,5-alpha-L-arabinofuranohexaose
+ 5 H2O = 6 alpha-L-arabinofuranose

Synonyms

ABF, ABF 2, Abf II, Abf III, ABF1, Abf2, ABF3, Abf4, Abf43A, Abf5, AbF51, Abf51A, Abf51B, Abf6, Abf62A, Abf62A-Axe6A, Abf62A-m2,3, AbfA, AbfATK4, AbfB, AbfD3, AbfI, AbfII, AbjA, acid arabinosidase, AF, AF2, AFase, Afase I, Afase II, AFQ, AFQ1, AFS, AFS1, AfuB, AkAbf, AkAbf54, AkabfA, AkAbfB, alpha-1,3-arabinofuranosidase, alpha-arabinofuranosidase, alpha-arabinofuranosidase B, alpha-arabinosidase, alpha-AraF, alpha-L-Abfase, Alpha-L-AF, alpha-L-Afase, alpha-L-arabinanase, alpha-L-arabinofuranosidase, alpha-L-arabinofuranosidase 54, alpha-L-arabinofuranosidase B, alpha-L-arabinofuranosidase D3, alpha-L-arabinofuranosidase II, alpha-L-arabinofuranosidase III, alpha-L-arabinofuranosidase/beta-D-xylosidase, alpha-L-arabinofuranoside arabinofuranohydrolase, alpha-L-arabinofuranoside hydrolase, alpha-L-arabinosidase, alpha-L-Araf, alpha-L-arafase, alpha-L-arafase I, alpha-L-arafase II, AN7908, AoAra54A, ARA-I, AraB, arabinofuranosidase, arabinofuranosidase 3, arabinofuranosidase 43A, arabinofuranosyl hydrolase, arabinosidase, arabinoxylan alpha-L-1,3-arabinofuranohydrolase, arabinoxylan alpha-L-arabinofuranohydrolase, arabinoxylan arabinofuranohydrolase, arabinoxylan hydrolase, AraF, Araf 51, Araf-ase, AraF43, Araf43A, Araf51A, Arb43A, Arb93A, ARF, Arfase, AtBXL1, AvAbfB, AXH, AXH-d3, Axh43A, Axh43B, AXS5, Axy43A, beta-D-galactofuranosidase 1, beta-D-galactofuranosidase 3, beta-D-galactofuranosidase 4, beta-D-xylosidase/alpha-L-arabinofuranosidase, beta-D-xylosidase/alpha-L-arabinosidase, beta-xylosidase/alpha-arabinofuranosidase, beta-xylosidase/alpha-arabinosidase, BlAraf, blArafA, BsAXH-m2,3, CcAbf62A, ccxyl3a, Csac_2411, Ct43Araf, CtCBM6B, CtGH43, EC 3.2.1.79, exo-1,5-alpha-L-arabinanase, exo-1,5-alpha-L-arabinofuranosidase, exo-alpha-L-arabinanase, exo-alpha-L-arabinofuranosidase, exo-arabinanase 43A, family 54 alpha-L-arabinofuranosidase, Galf-ase, GH 51 Abf, GH family 54 alpha-L-arabinofuranosidase, GH43 alpha-L-arabinofuranosidase, GH43B6, GH43_22 alpha-L-arabinofuranosidase, GH51 alpha-arabinofuranosidase, GH51 alpha-L-arabinofuranosidase, GH51 arabinofuranosidase, GH54 alpha-L-arabinofuranosidase, GH62, GH62 alpha-L-arabinofuranosidase, GRMZM2G077299, GRMZM2G120132, GRMZM2G180889, GRMZM2G393002, Hore_20580, L-arabinofuranosidase, L-arabinosidase, L213A variant beta-glycosidase, More, ORF0232, ORF2125, ORF2812, PaAbf62A, PcABF43A, PfABF62a, PfABF62b, PfABF62c, PoAbf, polysaccharide alpha-L-arabinofuranosidase, rArfA, RuXyn1, ScAraf62A, STX-IV, swu62A, Theth_1120, TmAFase, Tx-Abf, UmAbf62A, xarB, XYL3, XylC, XynD, XynF

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.55 non-reducing end alpha-L-arabinofuranosidase

Cloned

Cloned on EC 3.2.1.55 - non-reducing end alpha-L-arabinofuranosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 genes, abf51A and abf62A, transposon mutagenesis
-
a histidine tag is introduced at the N-terminal end of AbfATK4, and the recombinant protein is expressed in Escherichia coli BL21, under control of isopropyl-beta-D-thiogalactopyranoside-inducible T7 promoter
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ABF2 gene cloned and expressed in Saccharomyces cerevisiae
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Abf62A-Axe6A is a modular enzyme comprising (in order from the N-terminus): an N-terminal signal peptide, a glycoside hydrolase family 62 (GH62) catalytic module, a family 6 carbohydrate binding module (CBM6), a dockerin module and an additional carbohydrate esterase family 6 catalytic module (CE6). Three Abf62A-Axe6A derivatives are constructed, overexpressed in Escherichia coli, purified, and biochemically characterized: 1. RjAbf62A-Axe6A, containing all four modules but lacking the signal peptide, 2. RjAbf62A-CBM6, containing the GH62 and CBM6 modules, and 3. RjAxe6A, containing only CE6
-
abfB gene, nucleotide sequence determined
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alpha-AF cDNA cloned from Trichoderma reesei RUT C-30 chromosomal DNA using PCR technique, trying to express this gene in the yeast Schizosaccharomyces pombe, expression in Saccharomyces cerevisiae
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alpha-L-arabinofuranosidase is expressed in combination with either the beta-glucosidases from Saccharomycopsis fibuligera or from Aspergillus kawachii in Saccharomyces cerevisiae strain VIN13
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arfI and arfII, genes encoding alpha-L-arabinofuranosidase
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cDNA clones isolated
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cDNA isolation
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cloning and sequencing of the gene stxIV encoding alpha-L-arabinofuranosidase, expressed in Escherichia coli JM109
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cloning of the abfA T-6 gene, PCR, T7 polymerase expression vector pET9d, expressed in Escherichia coli BL21(DE3)
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constitutive recombinant expression of His-tagged enzyme in Escherichia coli strain MC1061
determination of the genetic interactions between AtBXL1 and known MSC-related transcription factors, overview
DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme without the N-terminal signal peptide in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, encoded on chromosome 2H
DNA and amino acid sequence determination and analysis, overexpression of the C-terminally His-tagged enzyme in Lactococcus lactis NZ9000 under control of nisin-inducible nisA promotor
DNA and amino acid sequence determination and analysis, sequence comparisons
exprerssion in Pichia pastoris
expressed in Escherichia coli
expressed in Escherichia coli Arctic cells
expressed in Escherichia coli BL21 DE3 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Codon Plus cells
expressed in Escherichia coli BL21(DE3) pLysS cells
expressed in Escherichia coli BL21(DE3)DELTASlyD cells
expressed in Escherichia coli BL21(lambdaDE3) cells
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells
expressed in Escherichia coli BL21gold (DE3) cells
-
expressed in Escherichia coli ER2566 cells
-
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Pichia pastoris
expressed in Pichia pastoris cells
-
expressed in Pichia pastoris GS115 cells
expressed in Pichia pastoris X-33 cells
expression in Aspergillus oryzae, GH43 alpha-L-arabinofuranosidase
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expression in Escherichia coli
expression in Escherichia coli and Pichia pastoris
-
expression in Escherichia coli BL21 (DE3)
expression in Escherichia DH5 alpha, phylogentic analyses
expression in Pichia pastori as an active enzyme accumulated in the culture broth, wild-type and mutant enzymes lacking one or both asparagine residues in the N-glycosylation sites N83Q, N202Q and N83Q/N202Q
-
expression in Pichia pastoris
expression in Saccharomyces cerevisiae
expression of His6-tagged enzyme in Pichia pastoris strain X33
expression of wild-type and mutant enzymes in Escherichia coli BL21(DE3)
functional recombinant expression of full-length enzyme and isolated catalytic module CtGH43 in Escherichia coli strain BL21(DE3) pLysS
gene abf51A amplified by PCR
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gene abfA encoding alpha-L-arabinofuranosidase homologously expressed in Streptomyces lividans by complementation of mutant IAF10-164 and DNA sequence determined, multicopy vector pIJ702 permits overproduction of the enzyme
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gene abfA, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain M15(pREP4), subcloning in Escherichia coli strain DH5alpha
gene abfB, DNA and amino acid sequence determination and analysis, recombinant extracellular expression of His-tagged enzyme in Aspergillus vadensis strain CBS 113365 using gpdA promoter from Aspergillus vadensis, method development and evaluation, overview
-
gene AbfD3 isolated, inserted into pET24 vector and expressed in Escherichia coli
-
gene afq1, overexpression of the enzyme containing thioredoxin, a histidine tag and a S-tag, attached to the N-terminus in Escherichia coli strain BL21(DE3)
gene afq1, semi-quantitative RT-PCR expression analysis, recombinant expression in and secretion from Escherichia coli
gene afs1, overexpression of the enzyme containing thioredoxin, a His-tag and a S-tag, attached to the N-terminus in Escherichia coli strain BL21(DE3)
gene afs1, semi-quantitative RT-PCR expression analysis, recombinant expression in and secretion from Escherichia coli
gene BSU28720, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3)
gene cloning of 2 isoenzymes, PCR products subcloned into pCR2.1 plasmids
-
gene CsAra, DNA and amino acid sequence determination and analysis, sequence comparisons
gene fg03054.1, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain TOP10
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gene Pcabf43A, semi-quantitative RT-PCR expression analysis
gene poabf, DNA and amino acid sequence determination and analysis, recombinant expression of the enzyme in Pichia pastoris and Kluyveromyces lactis and secretion to the culture medium with higher activity in Pichia pastoris
gene poabf, screening of a genetic library after random mutagenesis using error prone PCR system, recombinant expression of wild-type and mutant enzymes in Pichia pastoris using vector pSAL4
gene UM04309.1, expression of His6-tagged enzyme in Pichia pastoris strain X33
gene xylB encoding AF, heterologously cloned in Escherichia coli
-
gene, encoding AF, heterologously cloned in Escherichia coli
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gene, encoding AF, heterologously expressed in Escherichia coli JM83
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genes FaAra1, FaAra2 and FaAra3, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
genome analysis, and identification and cloning of genes PfabfB1, PfabfB2, PfabfB3, and PfabfB4, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH10B, functional expression of the enzymes in Saccharomyces cerevisiae with secretion to the medium
-
GH51 alpha-L-arabinofuranosidase
high-level expression in Escherichia coli BL21-CodonPlus (DE3)-RIL
-
individual expression of the GH43 and CBM6 modules in Escherichia coli
isozymes PfABF62a, PfABF62b, and PfABF62c, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged wild-type isozymes and isozyme pfabf62c mutant pfabf62cDELTACBM in Pichia pastoris strain GS115, subcloning in Escherichia coli
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N-terminus (N_term, GH43, and CBM22) and C-terminus (C_term, GH43, and CBM6) of XynF are cloned and expressed separately
orf0232, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus
orf2125, DNA and amino acid sequence determination and analysis, recombinant expression of N-termially 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus
orf2812, DNA and amino acid sequence determination and analysis, recombinant expression of N-termially 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus
overexpression in Escherichia coli
overexpression in Escherichia coli BL21
overexpression in Escherichia coli BL21(DE3)
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
overexpression of wild-type and mutant enzymes in Escherichia coli BL21(DE3)
recombinant expression of the enzyme in Escherichia coli
recombinant expression of wild-type and mutant enzymes in Streptomyces lividans strain 1326 and secretion to the culture medium
truncated 903 bp gene encoding the N-terminus of CtGH43 is part of a full-length family 43 glycoside hydrolase Ct43Araf which also contains two tandem family 6 carbohydrate-binding modules, CtCBM6A (405 bp) and CtCBM6B (402 bp), at the C-terminus, recombinant expression of the His6-tagged truncated enzyme in Escherichia coli strain BL21(DE3) pLysS
XS gene cloned and expressed in Escherichia coli, the recombinant 60 kDa protein shows XS and AF activities
-
xynC gene, encoding AF, heterologously expressed in Escherichia coli
-
xynD is isolated from genomic DNA of Bacillus subtilis subsp. subtilis ATCC 6051 and cloned for cytoplasmatic expression in Escherichia coli