3.2.1.55: non-reducing end alpha-L-arabinofuranosidase This is an abbreviated version! For detailed information about non-reducing end alpha-L-arabinofuranosidase, go to the full flat file .
Reaction
1,5-alpha-L-arabinofuranohexaose +
5 H2O
=
6 alpha-L-arabinofuranose
Synonyms ABF, ABF 2, Abf II, Abf III, ABF1, Abf2, ABF3, Abf4, Abf43A, Abf5, AbF51, Abf51A, Abf51B, Abf6, Abf62A, Abf62A-Axe6A, Abf62A-m2,3, AbfA, AbfATK4, AbfB, AbfD3, AbfI, AbfII, AbjA, acid arabinosidase, AF, AF2, AFase, Afase I, Afase II, AFQ, AFQ1, AFS, AFS1, AfuB, AkAbf, AkAbf54, AkabfA, AkAbfB, alpha-1,3-arabinofuranosidase, alpha-arabinofuranosidase, alpha-arabinofuranosidase B, alpha-arabinosidase, alpha-AraF, alpha-L-Abfase, Alpha-L-AF, alpha-L-Afase, alpha-L-arabinanase, alpha-L-arabinofuranosidase, alpha-L-arabinofuranosidase 54, alpha-L-arabinofuranosidase B, alpha-L-arabinofuranosidase D3, alpha-L-arabinofuranosidase II, alpha-L-arabinofuranosidase III, alpha-L-arabinofuranosidase/beta-D-xylosidase, alpha-L-arabinofuranoside arabinofuranohydrolase, alpha-L-arabinofuranoside hydrolase, alpha-L-arabinosidase, alpha-L-Araf, alpha-L-arafase, alpha-L-arafase I, alpha-L-arafase II, AN7908, AoAra54A, ARA-I, AraB, arabinofuranosidase, arabinofuranosidase 3, arabinofuranosidase 43A, arabinofuranosyl hydrolase, arabinosidase, arabinoxylan alpha-L-1,3-arabinofuranohydrolase, arabinoxylan alpha-L-arabinofuranohydrolase, arabinoxylan arabinofuranohydrolase, arabinoxylan hydrolase, AraF, Araf 51, Araf-ase, AraF43, Araf43A, Araf51A, Arb43A, Arb93A, ARF, Arfase, AtBXL1, AvAbfB, AXH, AXH-d3, Axh43A, Axh43B, AXS5, Axy43A, beta-D-galactofuranosidase 1, beta-D-galactofuranosidase 3, beta-D-galactofuranosidase 4, beta-D-xylosidase/alpha-L-arabinofuranosidase, beta-D-xylosidase/alpha-L-arabinosidase, beta-xylosidase/alpha-arabinofuranosidase, beta-xylosidase/alpha-arabinosidase, BlAraf, blArafA, BsAXH-m2,3, CcAbf62A, ccxyl3a, Csac_2411, Ct43Araf, CtCBM6B, CtGH43, EC 3.2.1.79, exo-1,5-alpha-L-arabinanase, exo-1,5-alpha-L-arabinofuranosidase, exo-alpha-L-arabinanase, exo-alpha-L-arabinofuranosidase, exo-arabinanase 43A, family 54 alpha-L-arabinofuranosidase, Galf-ase, GH 51 Abf, GH family 54 alpha-L-arabinofuranosidase, GH43 alpha-L-arabinofuranosidase, GH43B6, GH43_22 alpha-L-arabinofuranosidase, GH51 alpha-arabinofuranosidase, GH51 alpha-L-arabinofuranosidase, GH51 arabinofuranosidase, GH54 alpha-L-arabinofuranosidase, GH62, GH62 alpha-L-arabinofuranosidase, GRMZM2G077299, GRMZM2G120132, GRMZM2G180889, GRMZM2G393002, Hore_20580, L-arabinofuranosidase, L-arabinosidase, L213A variant beta-glycosidase, More, ORF0232, ORF2125, ORF2812, PaAbf62A, PcABF43A, PfABF62a, PfABF62b, PfABF62c, PoAbf, polysaccharide alpha-L-arabinofuranosidase, rArfA, RuXyn1, ScAraf62A, STX-IV, swu62A, Theth_1120, TmAFase, Tx-Abf, UmAbf62A, xarB, XYL3, XylC, XynD, XynF
ECTree
Temperature Stability
Temperature Stability on EC 3.2.1.55 - non-reducing end alpha-L-arabinofuranosidase
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20 - 60
the enzyme loses all activity after 1 h at 60°C. 60% activity remain after 1 h at 20-50°C
30 - 40
purified recombinant enzyme, pH 5.0, completely stable for at least 1 week
30 - 90
-
stable, half-life 12 h at 70°C, 2 h at 90°C
35 - 50
the enzyme loses 40% of its activity at 35°C and 90% at 50°C after 30 min of incubation
40 - 60
after 1 h pre-incubation of the enzyme in 20 mM Na-acetate buffer (pH 5.0), the enzyme is stable up to 40°C and loses all activity by treatment at 60°C
45 - 50
-
native crude enzyme, pH 7.0, over 90% activity remaining after 10 h
45 - 85
the enzyme maintains full activity at 50°C, 96% activity at 55°C, 60°C, and 65°C, and 80% activity at 70°C for 24 h
50 - 55
-
half-life of 2 h at 50°C and of 25 min at 55°C
50 - 60
-
enzyme is stable for 10 min at 50°C from pH 3-pH 8, at 60°C stable at pH 4 for 10 min, less stable at pH 5.5 and stable again at pH 7
50 - 80
-
stable up to 50°C, retains 50% original activity at 80°C
53 - 59
-
Ca2+ increases the melting temperature of the enzyme from 53°C to 59°C
53.5
melting temperature of the enzyme
55 - 70
purified native enzyme, stable up to 55°C for 30 min, half-lives at 55°C, 60°C, 65°C and 70°C are 167.4 min, 104.6 min, 28.1 min and 10.3 min, respectively
60 - 70
-
at 60°C, the enzyme retains over 90% activity after a 2-day incubation and over 60% activity after a 6-day incubation. The enzyme remains approximately 58% activity after a 1-day incubation at 70°C
60 - 80
-
at pH 7 enzyme is completely stable at 60°C for at least 80 min, retains 50% of its maximum activity after 75 min at 70°C and loses all activity after 15 min at 80°C
62
-
T50-value of mutant enzyme N83Q is 62.2°C
65 - 80
-
the half-lives of the enzyme at 65°C, 70°C, 75°C and 80°C were 1700 h, 390 h, 49 h and 0.4 h, respectively
68.5
melting temperatue, presence of 2 mM Mn2+
70 - 72
-
the wild type enzyme retains about 20% activity after 100 min at 70°C, about 20% activity after 40 min at 71°C, and less than 5% activity after 30 min at 72°C
70 - 75
-
the half-lives of the enzyme at 70°C and 75°C are 100 h and 90 h, respectively
70 - 85
thermal inactivation of the purified enzyme follows first-order kinetics with half-lives of 130, 41, 2.5, and 0.12 h at 70°C, 75°C, 80°C, and 85°C, respectively
70 - 90
-
remarkably thermostable, half of its activity remains after 0.7 h at 90°C
76
melting temperature, presence of 4 M NaCl and Mn2+ at pH 6.5
85
-
recombinant arfB gene product, stable up to
30
-
stable up to
30
more than 80% activity is retained after incubation at 30°C for 60 min
30
purified recombinant enzyme, 60 min, stable up to
30
purified native enzyme, pH 5.0, 1 h, more than 90% of the initial activity remains
30 - 45
the enzyme is stable up to 30°C for 3 h, and it retains 83% of its maximal activity at 40°C after 3 h of incubation. However, the stability of the enzyme decreases rapidly at temperature above 45°C
30 - 45
-
the enzyme retains about 70% activity after 100 min at 30-35°C and about 10% activity after 100 min at 40-45°C
40
stable up to
40
-
the recombinant enzyme retains more than 80% relative activity after 1 h of incubation at pH 7.0-8.5 below 40°C
40
purified wild-type and mutant enzymes, pH 5.0, loss of over 90% and 50% activity after 24 h, respectively
40
half-life for wild-type 16 days, for mutant S160G 7 days
40
purified recombinant enzyme, 60 min, 90% activity remaining
40
purified native enzyme, pH 5.0, 1 h, loss of 70% activity
40 - 50
the enzyme exhibits stability at temperatures up to 50°C, retaining 90% of its activity after pre-incubation at 40°C for 30 min. Higher temperatures result in a complete decrease in the enzyme activity
40 - 50
the enzyme is relatively stable at 40°C, but loses 76% of its activity after 10 min at 45°C, and 97% of activity at 50°C. The irreversible thermal denaturation half-time of the enzyme is calculated to be 112 min at 40°C, and 5.8 min at 45°C
45
-
stable up to 45°C
45
-
relatively stable up to
45
-
the enzyme loses all activity after 10 min incubation at 45°C and retains about 40% activity after 1 h at 37°C
45
the enzyme keeps about 40% of its initial activity after 20 min at 45°C
50
-
stable up to
50
-
purified recombinant His-tagged enzyme, 15 min, stable up to
50
-
1 h, complete loss of activity of isozyme ABF1, 37% remaining activity of isozyme ABF2
50
-
enzyme activity is stable under 50°C
50
purified recombinant enzyme, pH 5.0, stable up to
50
purified recombinant enzyme, pH 5.0, half-life is 17 h
50
purified wild-type and mutant enzymes, pH 5.0, loss of 75% and 55% activity after 23 h, respectively
50
-
after 3 h incubation isoenzyme C1 loses 90% of its original activity, C2 loses only 40%
50
purified recombinant enzyme, 60 min, 40% activity remaining
50
ABF 2, when incubated at 50°C, retains over 50% of its activity after 2 h
55
AbfA has a half-life of about 17.6 min at 55°C
55
the enzyme retains 0.03% activity after 1 h of incubation at 55°C
55
-
the enzyme retains 73% activity after 1 h of incubation at 55°C
55
-
native crude enzyme, pH 7.0, half-life is 10 h
60
-
the stability and activity of the enzyme declines sharply at temperatures above 60°C
60
-
T50-value of mutant enzyme N202Q is 60.1°C
60
-
T50-value of mutant enzyme N83Q/N202Q is 60.1°C
60
-
half-life time 1.5 h, inactivation after 2 h at 70°C
60
-
isoenzymes ABF1 and ABF3 seemed to be quite stable
60
-
purified recombinant His-tagged enzyme, 15 min, 77% activity remaining
60
40 min, complete and irreversible inactivation
60
purified native enzyme, 1 h, 75% activity remaining
60
purified recombinant enzyme, pH 5.0, half-life is a few min
60
purified wild-type and mutant enzymes, pH 5.0, loss of 75% and 70% activity after 5 min, respectively, almost inactivation after 10 min
60
-
loses activity completely after 10 min
60
purified recombinant enzyme, 60 min, no activity remaining
60
-
native crude enzyme, pH 7.0, 42% activity remaining after 10 h
60
-
the half-life of the enzyme at 60°C is approximately 2 h. The activity is stable over 7 h at 50°C and is slightly affected at 55°C with a half-life of 4 h. At 70°C, the enzyme is rapidly inactivated and has a half-life of 30 min
63
-
T50-value of wild-type enzyme is 63.3°C
65
pH 4-11, stable for 12 h
65
-
isoenzyme ABF2 retains 7% activity after 4 h
65
-
loss of 80% activity after 2 h
65
-
native crude enzyme, pH 7.0, 26% activity remaining after 10 h
70
-
isoenzymes ABF1 and ABF3, half-life 2 h and 5 h
70
-
purified recombinant His-tagged enzyme, 15 min, inactivation
70
Abf2 has a half-life of about 9.34 min at 70°C
70
-
AFase retains over 50% activity at 70°C
70
-
92% residual activity after 1h
75
-
50% loss of activity after 135 min
80
-
30 min, 10% loss of activity. Complete loss of activity after 80 min
90
-
half-life: 2 h
90
the enzyme shows no loss of activity after 20 h at 90°C