Literature summary for 3.2.1.55 extracted from

Remond, C.; Plantier-Royon, R.; Aubry, N.; Maes, E.; Bliard, C.; ODonohue, M.J.
Synthesis of pentose-containing disaccharides using a thermostable alpha-L-arabinofuranosidase (2004), Carbohydr. Res., 339, 2019-2025.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Thermobacillus xylanilyticus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	-	Thermobacillus xylanilyticus	5618	-

Organism

Organism	UniProt	Comment	Textmining
Thermobacillus xylanilyticus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
flash chromatography on silica gel columns	Thermobacillus xylanilyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.066	-	p-nitrophenol-beta-D-xylofuranoside	Thermobacillus xylanilyticus
0.19	-	p-nitrophenol-alpha-L-arabinopyranoside	Thermobacillus xylanilyticus
0.292	-	p-nitrophenol-beta-D-xylopyranoside	Thermobacillus xylanilyticus
465	-	p-nitrophenyo-alpha-L-arabinofuranoside	Thermobacillus xylanilyticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 p-nitrophenyl-alpha-L-arabinofuranoside	-	Thermobacillus xylanilyticus	p-nitrophenol + p-nitrophenyl-alpha-L-arabinofuranosyl-(1,2)-alpha-L-arabinofuranoside	-	?
2 p-nitrophenyl-beta-D-xylopyranoside	-	Thermobacillus xylanilyticus	p-nitrophenol + p-nitrophenyl-beta-D-xylopyranosyl-(1,2)-beta-D-xylopyranoside	-	?
2 p-nitrophenyl-beta-D-xylopyranoside	-	Thermobacillus xylanilyticus	p-nitrophenol + p-nitrophenyl-beta-D-xylopyranosyl-(1,3)-beta-D-xylopyranoside	-	?
p-nitrophenyl alpha-L-arabinofuranoside + benzyl-alpha-D-xylopyranoside	-	Thermobacillus xylanilyticus	?	-	?
p-nitrophenyl-alpha-L-arabinopyranoside + H2O	-	Thermobacillus xylanilyticus	p-nitrophenol + alpha-L-arabinopyranose	-	?
p-nitrophenyl-beta-D-xylofuranoside + H2O	-	Thermobacillus xylanilyticus	p-nitrophenol + beta-D-xylofuranose	-	?

Synonyms

Synonyms	Comment	Organism
alpha-L-arabinofuranosidase	Abf	Thermobacillus xylanilyticus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Thermobacillus xylanilyticus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.8	-	-	Thermobacillus xylanilyticus

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Release 2023.1 (January 2023)