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Literature summary for 3.2.1.55 extracted from
- A family GH51 alpha-L-arabinofuranosidase from Pleurotus ostreatus: identification, recombinant expression and characterization (2012), Appl. Microbiol. Biotechnol., 94, 995-1006.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene poabf, DNA and amino acid sequence determination and analysis, recombinant expression of the enzyme in Pichia pastoris and Kluyveromyces lactis and secretion to the culture medium with higher activity in Pichia pastoris | Pleurotus ostreatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Pleurotus ostreatus | |
| 0.64 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | recombinant enzyme, pH 5.0, 40°C | Pleurotus ostreatus |  |
| 3.79 | - |
4-nitrophenyl-beta-D-glucopyranoside | recombinant enzyme, pH 5.0, 40°C | Pleurotus ostreatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Pleurotus ostreatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81500 | - |
1 * 81500, SDS-PAGE | Pleurotus ostreatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pleurotus ostreatus | G0TES6 | type: Florida, ATCC MYA-2306, gene poabf | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | a single O-glycosylation site at the level of Ser160, mass spectrometric analysis | Pleurotus ostreatus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant extracellular enzyme from Pichia pastoris culture supernatant | Pleurotus ostreatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | strain solid state fermentation on tomato pomace, method evaluation, overview | Pleurotus ostreatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Pleurotus ostreatus | 4-nitrophenol + L-arabinofuranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | low activity | Pleurotus ostreatus | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| additional information | the enzyme is highly specific for alpha-L-arabinofuranosyl linkages. No activity ith 4-nitrophenyl-beta-D-xylopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, and 2-nitrophenylbeta-D-galactopyranoside | Pleurotus ostreatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 81500, SDS-PAGE | Pleurotus ostreatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-arabinofuranosidase | - |
Pleurotus ostreatus |
| PoAbf | - |
Pleurotus ostreatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pleurotus ostreatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | activity range, profile overview | Pleurotus ostreatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | purified recombinant enzyme, pH 5.0, completely stable for at least 1 week | Pleurotus ostreatus |
| 50 | - |
purified recombinant enzyme, pH 5.0, half-life is 17 h | Pleurotus ostreatus |
| 60 | - |
purified recombinant enzyme, pH 5.0, half-life is a few min | Pleurotus ostreatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.155 | - |
4-nitrophenyl-beta-D-glucopyranoside | recombinant enzyme, pH 5.0, 40°C | Pleurotus ostreatus | |
| 50.17 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | recombinant enzyme, pH 5.0, 40°C | Pleurotus ostreatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Pleurotus ostreatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | activity range, profile overview | Pleurotus ostreatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the purified recombinant enzyme shows a very high stability in a broad range of pH | Pleurotus ostreatus |
| 3 | - |
purified recombinant enzyme, 40°C, half-life is 24 days | Pleurotus ostreatus |
| 5 | - |
purified recombinant enzyme, 40°C, half-life is 51 days | Pleurotus ostreatus |
| 7 | - |
purified recombinant enzyme, 40°C, half-life is 42 days | Pleurotus ostreatus |
| 8 | - |
purified recombinant enzyme, 40°C, half-life is 38 days | Pleurotus ostreatus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to glycosyl hydrolase family 51, GH51 | Pleurotus ostreatus |
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