Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Thermobacillus xylanilyticus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor-diffusion method, inactive mutant in complex with a pentasaccharide, X-ray diffraction structure determination and analysis at 2.0 A resolution, space group P 65 2 2, seleno-methionyl derivative of wild-type arabinofuranosidase, X-ray diffraction structure determination and analysis at 2.2 A resolution, space group P 65 2 2, sequence identities and homologies, phylogentic analyses | Thermobacillus xylanilyticus |
Protein Variants | Comment | Organism |
---|---|---|
C180A | site-directed mutagenesis, reduced thermostability | Thermobacillus xylanilyticus |
E176Q | site-directed mutagenesis, inactive mutant | Thermobacillus xylanilyticus |
M94_I103delinsGG | site-directed mutagenesis, 70fold less active than the wild-type enzyme | Thermobacillus xylanilyticus |
W99A | site-directed mutagenesis, the arabinosyl moiety is not correctly bound in the active site when Trp99 is replaced by Ala | Thermobacillus xylanilyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
p-nitrophenyl-alpha-L-arabinofuranose | Tx-Abf, 60°C | Thermobacillus xylanilyticus | |
3.2 | - |
p-nitrophenyl-alpha-L-arabinofuranose | Tx-Abf-C180A mutant | Thermobacillus xylanilyticus | |
10 | - |
p-nitrophenyl-alpha-L-arabinofuranose | Tx-Abf-M94_I103delinsGG mutant | Thermobacillus xylanilyticus | |
45 | - |
p-nitrophenyl-alpha-L-arabinofuranose | Tx-Abf-W99A mutant | Thermobacillus xylanilyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | Thermobacillus xylanilyticus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobacillus xylanilyticus | O69262 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermobacillus xylanilyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.5 | - |
Tx-Abf-W99A mutant, 60°C, p-nitrophenyl-alpha-L-arabinofuranose | Thermobacillus xylanilyticus |
27 | - |
Tx-Abf-C180A mutant, 60°C, p-nitrophenyl-alpha-L-arabinofuranose | Thermobacillus xylanilyticus |
169 | - |
Tx-Abf, 60°C, p-nitrophenyl-alpha-L-arabinofuranose | Thermobacillus xylanilyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Thermobacillus xylanilyticus | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Thermobacillus xylanilyticus | 4-nitrophenol + L-arabinose | - |
? | |
xylan + H2O | - |
Thermobacillus xylanilyticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | dimer of trimers | Thermobacillus xylanilyticus |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-arabinofuranosidase | - |
Thermobacillus xylanilyticus |
Tx-Abf | - |
Thermobacillus xylanilyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
p-nitrophenyl-alpha-L-arabinofuranose | Tx-Abf-C180A mutant, 60°C | Thermobacillus xylanilyticus | |
35 | - |
p-nitrophenyl-alpha-L-arabinofuranose | Tx-Abf-M94_I103delinsGG mutant, 60°C | Thermobacillus xylanilyticus | |
165 | - |
p-nitrophenyl-alpha-L-arabinofuranose | Tx-Abf-W99A mutant, 60°C | Thermobacillus xylanilyticus | |
355 | - |
p-nitrophenyl-alpha-L-arabinofuranose | Tx-Abf, 60°C | Thermobacillus xylanilyticus |