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Literature summary for 3.2.1.55 extracted from

  • Paes, G.; Skov, L.K.; ODonohue, M.J.; Remond, C.; Kastrup, J.S.; Gajhede, M.; Mirza, O.
    The structure of the complex between a branched pentasaccharide and Thermobacillus xylanilyticus GH-51 arabinofuranosidase reveals xylan-binding determinants and induced fit (2008), Biochemistry, 47, 7441-7451.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermobacillus xylanilyticus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor-diffusion method, inactive mutant in complex with a pentasaccharide, X-ray diffraction structure determination and analysis at 2.0 A resolution, space group P 65 2 2, seleno-methionyl derivative of wild-type arabinofuranosidase, X-ray diffraction structure determination and analysis at 2.2 A resolution, space group P 65 2 2, sequence identities and homologies, phylogentic analyses Thermobacillus xylanilyticus

Protein Variants

Protein Variants Comment Organism
C180A site-directed mutagenesis, reduced thermostability Thermobacillus xylanilyticus
E176Q site-directed mutagenesis, inactive mutant Thermobacillus xylanilyticus
M94_I103delinsGG site-directed mutagenesis, 70fold less active than the wild-type enzyme Thermobacillus xylanilyticus
W99A site-directed mutagenesis, the arabinosyl moiety is not correctly bound in the active site when Trp99 is replaced by Ala Thermobacillus xylanilyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf, 60°C Thermobacillus xylanilyticus
3.2
-
p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-C180A mutant Thermobacillus xylanilyticus
10
-
p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-M94_I103delinsGG mutant Thermobacillus xylanilyticus
45
-
p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-W99A mutant Thermobacillus xylanilyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylan + H2O Thermobacillus xylanilyticus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Thermobacillus xylanilyticus O69262
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermobacillus xylanilyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.5
-
Tx-Abf-W99A mutant, 60°C, p-nitrophenyl-alpha-L-arabinofuranose Thermobacillus xylanilyticus
27
-
Tx-Abf-C180A mutant, 60°C, p-nitrophenyl-alpha-L-arabinofuranose Thermobacillus xylanilyticus
169
-
Tx-Abf, 60°C, p-nitrophenyl-alpha-L-arabinofuranose Thermobacillus xylanilyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Thermobacillus xylanilyticus 4-nitrophenol + alpha-L-arabinofuranose
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Thermobacillus xylanilyticus 4-nitrophenol + L-arabinose
-
?
xylan + H2O
-
Thermobacillus xylanilyticus ?
-
?

Subunits

Subunits Comment Organism
hexamer dimer of trimers Thermobacillus xylanilyticus

Synonyms

Synonyms Comment Organism
alpha-L-arabinofuranosidase
-
Thermobacillus xylanilyticus
Tx-Abf
-
Thermobacillus xylanilyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24
-
p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-C180A mutant, 60°C Thermobacillus xylanilyticus
35
-
p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-M94_I103delinsGG mutant, 60°C Thermobacillus xylanilyticus
165
-
p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-W99A mutant, 60°C Thermobacillus xylanilyticus
355
-
p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf, 60°C Thermobacillus xylanilyticus