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Literature summary for 3.2.1.55 extracted from

  • Canakci, S.; Belduz, A.O.; Saha, B.C.; Yasar, A.; Ayaz, F.A.; Yayli, N.
    Purification and characterization of a highly thermostable alpha-L-arabinofuranosidase from Geobacillus caldoxylolyticus TK4 (2007), Appl. Microbiol. Biotechnol., 75, 813-820.
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
a histidine tag is introduced at the N-terminal end of AbfATK4, and the recombinant protein is expressed in Escherichia coli BL21, under control of isopropyl-beta-D-thiogalactopyranoside-inducible T7 promoter Parageobacillus caldoxylosilyticus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, complete inhibition Parageobacillus caldoxylosilyticus
Hg2+ 1 mM, complete inhibition Parageobacillus caldoxylosilyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Parageobacillus caldoxylosilyticus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Parageobacillus caldoxylosilyticus
-
-
intracellular
-
 Parageobacillus caldoxylosilyticus 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates Parageobacillus caldoxylosilyticus
Mn2+ stimulates Parageobacillus caldoxylosilyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 4 * 59000, SDS-PAGE Parageobacillus caldoxylosilyticus
236000
-
 gel filtration Parageobacillus caldoxylosilyticus

Organism

Organism UniProt Comment Textmining
Parageobacillus caldoxylosilyticus
-
-
-
Parageobacillus caldoxylosilyticus TK4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Parageobacillus caldoxylosilyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no endoarabinanase activity Parageobacillus caldoxylosilyticus ?
-
 ?
additional information no endoarabinanase activity Parageobacillus caldoxylosilyticus TK4 ?
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Parageobacillus caldoxylosilyticus p-nitrophenol + L-arabinofuranose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Parageobacillus caldoxylosilyticus TK4 p-nitrophenol + L-arabinofuranose
-
 ?
p-nitrophenyl-beta-D-xylopyranoside + H2O low activity Parageobacillus caldoxylosilyticus p-nitrophenol + beta-D-xylopyranose
-
 ?
p-nitrophenyl-beta-D-xylopyranoside + H2O low activity Parageobacillus caldoxylosilyticus TK4 p-nitrophenol + beta-D-xylopyranose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 59000, SDS-PAGE Parageobacillus caldoxylosilyticus

Synonyms

Synonyms Comment Organism
AbfATK4
-
 Parageobacillus caldoxylosilyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75 80
-
 Parageobacillus caldoxylosilyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 65 96 h, stable Parageobacillus caldoxylosilyticus
70
-
 48 h, stable Parageobacillus caldoxylosilyticus
75
-
 12 h, stable Parageobacillus caldoxylosilyticus
80
-
 30 min, 10% loss of activity. Complete loss of activity after 80 min Parageobacillus caldoxylosilyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Parageobacillus caldoxylosilyticus