3.2.1.55: non-reducing end alpha-L-arabinofuranosidase

This is an abbreviated version!
For detailed information about non-reducing end alpha-L-arabinofuranosidase, go to the full flat file.

Reaction

Synonyms

ABF, ABF 2, Abf II, Abf III, ABF1, Abf2, ABF3, Abf4, Abf43A, Abf5, AbF51, Abf51A, Abf51B, Abf6, Abf62A, Abf62A-Axe6A, Abf62A-m2,3, AbfA, AbfATK4, AbfB, AbfD3, AbfI, AbfII, AbjA, acid arabinosidase, AF, AF2, AFase, Afase I, Afase II, AFQ, AFQ1, AFS, AFS1, AfuB, AkAbf, AkAbf54, AkabfA, AkAbfB, alpha-1,3-arabinofuranosidase, alpha-arabinofuranosidase, alpha-arabinofuranosidase B, alpha-arabinosidase, alpha-AraF, alpha-L-Abfase, Alpha-L-AF, alpha-L-Afase, alpha-L-arabinanase, alpha-L-arabinofuranosidase, alpha-L-arabinofuranosidase 54, alpha-L-arabinofuranosidase B, alpha-L-arabinofuranosidase D3, alpha-L-arabinofuranosidase II, alpha-L-arabinofuranosidase III, alpha-L-arabinofuranosidase/beta-D-xylosidase, alpha-L-arabinofuranoside arabinofuranohydrolase, alpha-L-arabinofuranoside hydrolase, alpha-L-arabinosidase, alpha-L-Araf, alpha-L-arafase, alpha-L-arafase I, alpha-L-arafase II, AN7908, AoAra54A, ARA-I, AraB, arabinofuranosidase, arabinofuranosidase 3, arabinofuranosidase 43A, arabinofuranosyl hydrolase, arabinosidase, arabinoxylan alpha-L-1,3-arabinofuranohydrolase, arabinoxylan alpha-L-arabinofuranohydrolase, arabinoxylan arabinofuranohydrolase, arabinoxylan hydrolase, AraF, Araf 51, Araf-ase, AraF43, Araf43A, Araf51A, Arb43A, Arb93A, ARF, Arfase, AtBXL1, AvAbfB, AXH, AXH-d3, Axh43A, Axh43B, AXS5, Axy43A, beta-D-galactofuranosidase 1, beta-D-galactofuranosidase 3, beta-D-galactofuranosidase 4, beta-D-xylosidase/alpha-L-arabinofuranosidase, beta-D-xylosidase/alpha-L-arabinosidase, beta-xylosidase/alpha-arabinofuranosidase, beta-xylosidase/alpha-arabinosidase, BlAraf, blArafA, BsAXH-m2,3, CcAbf62A, ccxyl3a, Csac_2411, Ct43Araf, CtCBM6B, CtGH43, EC 3.2.1.79, exo-1,5-alpha-L-arabinanase, exo-1,5-alpha-L-arabinofuranosidase, exo-alpha-L-arabinanase, exo-alpha-L-arabinofuranosidase, exo-arabinanase 43A, family 54 alpha-L-arabinofuranosidase, Galf-ase, GH 51 Abf, GH family 54 alpha-L-arabinofuranosidase, GH43 alpha-L-arabinofuranosidase, GH43B6, GH43_22 alpha-L-arabinofuranosidase, GH51 alpha-arabinofuranosidase, GH51 alpha-L-arabinofuranosidase, GH51 arabinofuranosidase, GH54 alpha-L-arabinofuranosidase, GH62, GH62 alpha-L-arabinofuranosidase, GRMZM2G077299, GRMZM2G120132, GRMZM2G180889, GRMZM2G393002, Hore_20580, L-arabinofuranosidase, L-arabinosidase, L213A variant beta-glycosidase, More, ORF0232, ORF2125, ORF2812, PaAbf62A, PcABF43A, PfABF62a, PfABF62b, PfABF62c, PoAbf, polysaccharide alpha-L-arabinofuranosidase, rArfA, RuXyn1, ScAraf62A, STX-IV, swu62A, Theth_1120, TmAFase, Tx-Abf, UmAbf62A, xarB, XYL3, XylC, XynD, XynF

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.55 non-reducing end alpha-L-arabinofuranosidase

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Results	in table
38	Activating Compound
10736	AA Sequence
142	Application
1	CAS Registry Number
138	Cloned(Commentary)
36	Crystallization (Commentary)
14	Expression
71	General Information
4	General Stability
2	IC50 Value
226	Inhibitors
61	kcat/KM [mM/s]
5	Ki Value [mM]
267	KM Value [mM]
90	Localization
60	Metals/Ions
268	Molecular Weight [Da]
272	Natural Substrates/ Products (Substrates)
7	Organic Solvent Stability
1014	Organism
1	Oxidation Stability
4	Pathway
161	PDB ID
218	pH Optimum
46	pH Range
71	pH Stability
20	pI Value
19	Posttranslational Modification
129	Protein Variants
183	Purification (Commentary)
17	Reaction
3	Reaction Type
611	Reference
57	Source Tissue
137	Specific Activity [micromol/min/mg]
7	Storage Stability
1841	Substrates and Products (Substrate)
218	Subunits
572	Synonyms
1	Systematic Name
195	Temperature Optimum [°C]
34	Temperature Range [°C]
110	Temperature Stability [°C]
157	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 3.2.1.55 - non-reducing end alpha-L-arabinofuranosidase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

5.5 - 6.7

1,5-alpha-L-arabinobiose

2 entries

4.8 - 6.9

1,5-alpha-L-arabinotriose

2 entries

0.5

2,3-di-alpha-L-arabinofuranosyl xylotriose

Acetivibrio clariflavus

-

at pH 6.5 and 60°C

749717

6.9

2-alpha-L-arabinofuranosyl xylotriose

Acetivibrio clariflavus

-

at pH 6.5 and 60°C

749717

22.8

3-alpha-L-arabinofuranosyl xylobiose

Acetivibrio clariflavus

-

at pH 6.5 and 60°C

749717

0.00015 - 6500

4-nitrophenyl alpha-L-arabinofuranoside

23 entries

205

4-nitrophenyl alpha-L-arabinopyranoside

Acetivibrio clariflavus

-

at pH 6.5 and 60°C

749717

0.19 - 38

4-nitrophenyl beta-D-galactofuranoside

2 entries

0.44

4-nitrophenyl beta-D-xylopyranoside

Paenibacillus sp.

AM283040

pH 6.5, 60°C

737909

0.005 - 795

4-nitrophenyl-alpha-L-arabinofuranoside

10 entries

0.067 - 63.6

alpha-1,5-L-arabinofuranobiose

5 entries

46600

alpha-1,5-L-arabinohexaose

Fusarium graminearum

-

pH 5.0, 40°C, wild-type enzyme

709012

20500

alpha-1,5-L-arabinopentaose

Fusarium graminearum

-

pH 5.0, 40°C, wild-type enzyme

709012

11300

alpha-1,5-L-arabinotetraose

Fusarium graminearum

-

pH 5.0, 40°C, wild-type enzyme

709012

31700

alpha-1,5-L-arabinotriose

Fusarium graminearum

-

pH 5.0, 40°C, wild-type enzyme

709012

0.35 - 2.425

rye arabinoxylan

3 entries

-

0.121 - 2.56

wheat arabinoxylan

4 entries

-

0.042

wheat L-arabinoxylan

Streptomyces coelicolor

O54161

pH 5.5, 37°C, recombinant wild-type enzyme

732171

-

5.5

1,5-alpha-L-arabinobiose

Levilactobacillus brevis

-

at pH 5.8 and 45°C

744883

6.7

1,5-alpha-L-arabinobiose

Weissella sp. 142

A0A1L7HA00

at pH 5.8 and 45°C

744883

4.8

1,5-alpha-L-arabinotriose

Levilactobacillus brevis

-

at pH 5.8 and 45°C

744883

6.9

1,5-alpha-L-arabinotriose

Weissella sp. 142

A0A1L7HA00

at pH 5.8 and 45°C

744883

0.00015

4-nitrophenyl alpha-L-arabinofuranoside

Streptomyces avermitilis

-

mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C

715550

0.015

4-nitrophenyl alpha-L-arabinofuranoside

Streptomyces avermitilis

-

mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C

715550

0.09

4-nitrophenyl alpha-L-arabinofuranoside

Streptomyces avermitilis

-

mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C

715550

0.2

4-nitrophenyl alpha-L-arabinofuranoside

Penicillium oxalicum

-

at pH 4.5 and 35°C

749551

0.22

4-nitrophenyl alpha-L-arabinofuranoside

Streptomyces avermitilis

-

wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C

715550

0.33

4-nitrophenyl alpha-L-arabinofuranoside

Streptomyces avermitilis

-

mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C

715550

0.8

4-nitrophenyl alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

O69262

mutant R69H/G179F/L352M, pH 7.0, 45°C

737311

0.8

4-nitrophenyl alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

O69262

mutant R69H/L352M, pH 7.0, 45°C

737311

1.2

4-nitrophenyl alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

O69262

mutant R69H/N216W/L352M, pH 7.0, 45°C

737311

1.32

4-nitrophenyl alpha-L-arabinofuranoside

Talaromyces purpureogenus

-

pH 4.6, 37°C

738310

4.14

4-nitrophenyl alpha-L-arabinofuranoside

Streptomyces avermitilis

-

mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C

715550

4.17

4-nitrophenyl alpha-L-arabinofuranoside

Talaromyces purpureogenus

C3W4Y2

in 50 mM sodium citrate buffer (pH 4.0), at 30°C

713826

11.7

4-nitrophenyl alpha-L-arabinofuranoside

uncultured rumen bacterium

E3TBJ3

in 50 mM phosphate/citrate buffer pH 7.0, at 40°C

715805

18

4-nitrophenyl alpha-L-arabinofuranoside

Thermomyces dupontii

-

in 50 mM potassium phosphate buffer (pH 7.0), at 50°C

715952

25.4

4-nitrophenyl alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

O69262

mutant R69H, pH 7.0, 45°C

737311

25.9

4-nitrophenyl alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

O69262

mutant L352M, pH 7.0, 45°C

737311

50

4-nitrophenyl alpha-L-arabinofuranoside

Alicyclobacillus sp.

A0A0P0ISK8

pH 6.0, 60°C

737905

205

4-nitrophenyl alpha-L-arabinofuranoside

Caldicellulosiruptor saccharolyticus

-

in 50 mM citric acid buffer (pH 5.5), at 80°C

715312

221

4-nitrophenyl alpha-L-arabinofuranoside

Caldicellulosiruptor saccharolyticus

-

in 50 mM citric acid buffer (pH 5.5), at 80°C

715312

556

4-nitrophenyl alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

O69262

wild-type, pH 7.0, 45°C

737311

811

4-nitrophenyl alpha-L-arabinofuranoside

Pseudothermotoga thermarum

F7YTI3

pH 5.0, 95°C

738475

1050

4-nitrophenyl alpha-L-arabinofuranoside

Paenibacillus sp.

AM283040

pH 6.5, 60°C

737909

6500

4-nitrophenyl alpha-L-arabinofuranoside

Caldicellulosiruptor sp. F32

-

at pH 6.0 and 80°C

744111

0.19

4-nitrophenyl beta-D-galactofuranoside

Talaromyces purpureogenus

-

pH 4.6, 37°C

738310

38

4-nitrophenyl beta-D-galactofuranoside

Paenibacillus sp.

AM283040

pH 6.5, 60°C

737909

0.005

4-nitrophenyl-alpha-L-arabinofuranoside

Ustilago maydis

A0A0D1BZQ4

pH 5.0, 37°C

732166

0.011

4-nitrophenyl-alpha-L-arabinofuranoside

Streptomyces coelicolor

O54161

pH 5.5, 37°C, recombinant wild-type enzyme

732171

0.062

4-nitrophenyl-alpha-L-arabinofuranoside

Podospora anserina

E2GHW5

pH 5.0, 37°C

732166

9.8

4-nitrophenyl-alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

-

mutant H98A/W99A, pH 5.8, 60°C

731785

15.1

4-nitrophenyl-alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

-

mutant W99A, pH 5.8, 60°C

731785

18

4-nitrophenyl-alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

-

mutant W99F, pH 5.8, 60°C

731785

28.2

4-nitrophenyl-alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

-

mutant H98F/W99F, pH 5.8, 60°C

731785

126

4-nitrophenyl-alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

-

mutant H98A, pH 5.8, 60°C

731785

228

4-nitrophenyl-alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

-

mutant H98F, pH 5.8, 60°C

731785

795

4-nitrophenyl-alpha-L-arabinofuranoside

Thermobacillus xylanilyticus

-

wild-type enzyme, pH 5.8, 60°C

731785

0.067

alpha-1,5-L-arabinofuranobiose

Streptomyces avermitilis

-

mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C

715550

0.18

alpha-1,5-L-arabinofuranobiose

Streptomyces avermitilis

-

mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C

715550

1.78

alpha-1,5-L-arabinofuranobiose

Streptomyces avermitilis

-

mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C

715550

11.15

alpha-1,5-L-arabinofuranobiose

Streptomyces avermitilis

-

mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C

715550

63.6

alpha-1,5-L-arabinofuranobiose

Streptomyces avermitilis

-

wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C

715550

0.35

rye arabinoxylan

Talaromyces funiculosus

-

pH 4.0, 40°C, isozyme PfABF62a

731728

-

1.91

rye arabinoxylan

Talaromyces funiculosus

-

pH 4.0, 40°C, isozyme PfABF62c

731728

-

2.425

rye arabinoxylan

Talaromyces funiculosus

-

pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM

731728

-

0.121

wheat arabinoxylan

Talaromyces funiculosus

-

pH 4.0, 40°C, isozyme PfABF62a

731728

-

0.64

wheat arabinoxylan

Talaromyces funiculosus

-

pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM

731728

-

0.92

wheat arabinoxylan

Talaromyces funiculosus

-

pH 4.0, 40°C, isozyme PfABF62c

731728

-

2.56

wheat arabinoxylan

Talaromyces funiculosus

-

pH 4.0, 40°C, isozyme PfABF62b

731728

-