Application | Comment | Organism |
---|---|---|
agriculture | simple and low cost saccharification of agricultural residues including waste can be performed under mild conditions and the reducing sugars obtained are useful for bioconversion to valueadded products | Streptomyces sp. PC22 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Streptomyces sp. PC22 | |
Fe2+ | concentration 1 mM, reduces the enzyme activity to less than 50% of that in the absence of the metal ion | Streptomyces sp. PC22 | |
Hg2+ | - |
Streptomyces sp. PC22 | |
Mn2+ | concentration 1 mM, reduces the enzyme activity to less than 50% of that in the absence of the metal ion | Streptomyces sp. PC22 | |
additional information | no effect with Ca2+ and Mg2+; no effect with iodoacetamide | Streptomyces sp. PC22 | |
N-bromosuccinimide | enzyme completely inactivated | Streptomyces sp. PC22 | |
Zn2+ | concentration 1 mM, reduces the enzyme activity to less than 50% of that in the absence of the metal ion | Streptomyces sp. PC22 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 6.0 | Streptomyces sp. PC22 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces sp. PC22 | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
404000 | - |
gel filtration, subunit mass of about 79000 Da, SDS-PAGE | Streptomyces sp. PC22 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. PC22 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
precipitation followed by column chromatography | Streptomyces sp. PC22 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Streptomyces sp. PC22 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | arabinofuranosidase shows a mild but significant synergistic effect in combination with other xylanolytic enzymes, including xylanase, beta-xylosidase and acetyl esterase, on the degradation of oat-spelt xylan, corn cob and corn husk substrates with a 1.25, 1.32 and 1.21fold increase in the amount of reducing sugar released | Streptomyces sp. PC22 | ? | - |
? | |
additional information | no activity with p-nitrophenyl-beta-D-xylopyranoside | Streptomyces sp. PC22 | ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. PC22 | p-nitrophenol + L-arabinofuranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular weight 404000, subunit mass about 79000 Da, SDS-PAGE | Streptomyces sp. PC22 |
Synonyms | Comment | Organism |
---|---|---|
AFase | - |
Streptomyces sp. PC22 |
alpha-L-arabinofuranosidase | - |
Streptomyces sp. PC22 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Streptomyces sp. PC22 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
AFase retains over 50% activity at 70°C | Streptomyces sp. PC22 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Streptomyces sp. PC22 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | 20% activity at pH 9.0 | Streptomyces sp. PC22 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | 20% activity at pH 9.0 | Streptomyces sp. PC22 |