3.2.1.28: alpha,alpha-trehalase
This is an abbreviated version!
For detailed information about alpha,alpha-trehalase, go to the full flat file.
Word Map on EC 3.2.1.28
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3.2.1.28
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sucrase
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lactase
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border
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larvae
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disaccharidase
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brush
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trehalose-6-phosphate
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glycogen
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midgut
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glucoamylase
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hemolymph
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camp-dependent
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validamycin
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chitin
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isomaltase
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silkworm
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alpha-glucosidase
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instar
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bombyx
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brush-border
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gamma-glutamyltransferase
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sucrase-isomaltase
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microvillar
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lepidoptera
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carbohydrases
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molitor
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tenebrio
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20-hydroxyecdysone
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diapause
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metarhizium
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cellobiase
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planthopper
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ascospores
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analysis
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diagnostics
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synthesis
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agriculture
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medicine
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biotechnology
- 3.2.1.28
- sucrase
- lactase
- border
- larvae
- disaccharidase
-
brush
- trehalose-6-phosphate
- glycogen
- midgut
- glucoamylase
- hemolymph
-
camp-dependent
- validamycin
- chitin
- isomaltase
- silkworm
- alpha-glucosidase
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instar
- bombyx
-
brush-border
- gamma-glutamyltransferase
- sucrase-isomaltase
- microvillar
- lepidoptera
-
carbohydrases
- molitor
- tenebrio
- 20-hydroxyecdysone
-
diapause
- metarhizium
- cellobiase
- planthopper
- ascospores
- analysis
- diagnostics
- synthesis
- agriculture
- medicine
- biotechnology
Reaction
Synonyms
acid (non regulatory) trehalase, acid non-regulatory trehalase, acid trehalase, alkaline trehalase, alpha,alpha glucoside 1-glucohydrolase, alpha,alpha trehalase, alpha,alpha trehalose glucohydrolase, alpha,alpha'-trehalose 1-D-glucohydrolase, Alpha,alpha-trehalase, Alpha,alpha-trehalose glucohydrolase, alpha-glucoside-1-glucohydrolase, ATC1, Atc1p, Ath1, ATHA, ATM1, GH37 trehalase, glucose alpha-1,1-glucose hydrolase, HaTre-1, HaTreh-1, Hl-Tre, membrane-bound SfTre2, MSMEG 4528, neutral regulatory trehalase, neutral trehalase, NT-INV, Ntc1p, NTH1, Nth1p, Nth2p, NTHA, Ntp1p, Px_EclTre, Saci_1816, SeTre-1, SeTre-2, SfTre1, soluble P I type trehalase, soluble P II type trehalase, soluble trehalase, THA, Tre-2, TRE1, Tre37A, TreA, treB, TreF, Treh, Treh1, Treh2, trehalase, trehalase-1, trehalase-invertase, TreZ
ECTree
3.2.1.28 alpha,alpha-trehalaseAdvanced search results
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results (Inhibitors
Inhibitors on EC 3.2.1.28 - alpha,alpha-trehalase
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
(1R,2R,3R,6S,7S,7aS)-3-(hydroxymethyl)-6-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]oxy]hexahydro-1H-pyrrolizine-1,2,7-triol
(2R,3R,4S,5R,6R)-2-[[(1R,2R,5R,6R,7R,7aR)-6,7-dihydroxy-1,5-bis(hydroxymethyl)hexahydro-1H-pyrrolizin-2-yl]oxy]-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
(2S,3R,4S,5S,6R)-2-[[(2R,5R,6R,7R,7aR)-6,7-dihydroxy-5-(hydroxymethyl)hexahydro-1H-pyrrolizin-2-yl]oxy]-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
1,3-bis((2R,3R,4R,5S)-3,4,5-trihydroxy-2-(hydroxymethyl)piperidin-1-yl)propane-1,3-dione
micromolar inhibitor
diethyldicarbonate
the compound modifies a His residue that results in a less active enzyme. After 40 min with 40 mM diethylpyrocarbonate at 30°C pH 6.0, trehalase activity decreases to about 50% of the initial activity
diphosphate
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more than 50% inhibition at 8 mM and complete inhibition at 16 mM
fructose
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10 mM, 45% inhibition. Inhibitory effect is abolished by Ca2+
lactose
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10 mM, 68% inhibition. Inhibitory effect is abolished by Ca2+
maltose
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10 mM, 54% inhibition. Inhibitory effect is abolished by Ca2+
NaCl
strong inhibition at 10 mM, treatment of cells leads to accumulation of trehalose
NH4Cl
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1 mM NH4Cl, 22% inhibition of extracellular enzyme, 27% inhibition of intracellular enzyme
(1R,2R,3R,6S,7S,7aS)-3-(hydroxymethyl)-6-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]oxy]hexahydro-1H-pyrrolizine-1,2,7-triol
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casuarine-6-O-alpha-D-glucoside, active against trehalases derived from insects, bacteria and eukaryotes
(1R,2R,3R,6S,7S,7aS)-3-(hydroxymethyl)-6-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]oxy]hexahydro-1H-pyrrolizine-1,2,7-triol
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casuarine-6-O-alpha-D-glucoside, active against trehalases derived from insects, bacteria and eukaryotes
(1R,2R,3R,6S,7S,7aS)-3-(hydroxymethyl)-6-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]oxy]hexahydro-1H-pyrrolizine-1,2,7-triol
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casuarine-6-O-alpha-D-glucoside, active against trehalases derived from insects, bacteria and eukaryotes
(2R,3R,4S,5R,6R)-2-[[(1R,2R,5R,6R,7R,7aR)-6,7-dihydroxy-1,5-bis(hydroxymethyl)hexahydro-1H-pyrrolizin-2-yl]oxy]-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
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7-homocasuarine-6-O-alpha-D-glucoside, active against trehalases derived from insects, bacteria and eukaryotes
(2R,3R,4S,5R,6R)-2-[[(1R,2R,5R,6R,7R,7aR)-6,7-dihydroxy-1,5-bis(hydroxymethyl)hexahydro-1H-pyrrolizin-2-yl]oxy]-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
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7-homocasuarine-6-O-alpha-D-glucoside, active against trehalases derived from insects, bacteria and eukaryotes
(2R,3R,4S,5R,6R)-2-[[(1R,2R,5R,6R,7R,7aR)-6,7-dihydroxy-1,5-bis(hydroxymethyl)hexahydro-1H-pyrrolizin-2-yl]oxy]-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
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7-homocasuarine-6-O-alpha-D-glucoside, active against trehalases derived from insects, bacteria and eukaryotes
(2S,3R,4S,5S,6R)-2-[[(2R,5R,6R,7R,7aR)-6,7-dihydroxy-5-(hydroxymethyl)hexahydro-1H-pyrrolizin-2-yl]oxy]-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
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7-deoxycasuarine-6-O-alpha-D-glucoside, active against trehalases derived from insects, bacteria and eukaryotes
(2S,3R,4S,5S,6R)-2-[[(2R,5R,6R,7R,7aR)-6,7-dihydroxy-5-(hydroxymethyl)hexahydro-1H-pyrrolizin-2-yl]oxy]-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
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7-deoxycasuarine-6-O-alpha-D-glucoside, active against trehalases derived from insects, bacteria and eukaryotes
(2S,3R,4S,5S,6R)-2-[[(2R,5R,6R,7R,7aR)-6,7-dihydroxy-5-(hydroxymethyl)hexahydro-1H-pyrrolizin-2-yl]oxy]-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
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7-deoxycasuarine-6-O-alpha-D-glucoside, active against trehalases derived from insects, bacteria and eukaryotes
1,10-phenanthroline
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inhibits the acid trehalase, inhibits the neutral trehalase in presence of 5 mM trehalose
1,10-phenanthroline
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inhibits the neutral trehalase in presence of 5 mM trehalose
1,2-bis((2R,3R,4R,5S)-3,4,5-trihydroxy-2-(hydroxymethyl)piperidin-1-yl)ethane-1,2-dione
micromolar inhibitor
1,2-bis((2R,3R,4R,5S)-3,4,5-trihydroxy-2-(hydroxymethyl)piperidin-1-yl)ethane-1,2-dione
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micromolar inhibitor
1,4-bis((2R,3R,4R,5S)-3,4,5-trihydroxy-2-(hydroxymethyl)piperidin-1-yl)butane-1,4-dione
micromolar inhibitor
1,4-bis((2R,3R,4R,5S)-3,4,5-trihydroxy-2-(hydroxymethyl)piperidin-1-yl)butane-1,4-dione
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micromolar inhibitor
acetate
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strong inhibition by acetic acid/acetate buffer, V-trehalase
ADP
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10 mM, 16% residual activity, extracellular isoform, 12% residual activity, intracellular isoform. In presence of CaCl2, 18% residual activity for extracellular isoform, 12% residual activity, intracellular isoform, respectively
amygdalin
i.e. glucose-beta-1,6-glucose-beta-mandelonitrile, linear competitive inhibitor
ATP
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trehalase of dormant spores is strongly inhibited by 0.5 mM ATP. Trehalase from germinating spores is not inhibited by ATP up to much higher ATP concentrations
ATP
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10 mM, 12% residual activity, extracellular isoform, 6% residual activity, intracellular isoform. In presence of CaCl2, 2% residual activity for extracellular isoform, 10% residual activity, intracellular isoform
ATP
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at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP
ATP
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at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP
castanospermine
effective competitive inhibitor of trehalase, completely inhibited by 0.001 mM
castanospermine
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effective competitive inhibitor of trehalase, completely inhibited by 0.001 mM
cellobiose
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10 mM, 54% inhibition. Inhibitory effect is abolished by Ca2+
Cu2+
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1 mM CuSO4, complete inhibition of extracellular enzyme, 47% inhibition of intracellular enzyme
D-glucose
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isoform Atc1p is subject to glucose repression, but exhaustion of glucose itself does not increase the activity
EDTA
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1 mM, 30% inhibition of extracellular enzyme, 54% inhibition of intracellular enzyme
EDTA
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1 mM, 70% residual activity for extracellular isoform, 46% residual activity, intracellular isoform
EDTA
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at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP
EDTA
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at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP
glucose
the enzyme is regulated by glucose-induced catabolite repression
Hg2+
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the glycoprotein enzyme forms are more susceptible than the nonglycoprotein forms
MDL 25 637
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i.e. 7-O-beta-D-glucopyranosyl-alpha-homojirimycin; potent, reversible, competitive, slow-binding nature
MDL 25 637
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i.e. 7-O-beta-D-glucopyranosyl-alpha-homojirimycin; potent, time-dependent
methyl-alpha-glucoside
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slight inhibition of acid trehalase
phloretin
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inhibits the acid trehalase, inhibits the neutral trehalase in presence of 20 mM trehalose
phloretin
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inhibits the acid trehalase, inhibits the neutral trehalase in presence of 20 mM trehalose
phlorizin
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hyperbolic uncompetitive, binds only to enzyme-substrate complex
prunasin
i.e glucose-beta-mandelonitrile, linear competitive inhibitor
sucrose
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10 mM, 54% inhibition. Inhibitory effect is abolished by Ca2+
validamycin A
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potent, reversible, competitive, slow-binding nature
validamycin A
validamycin A binds at the active site with multiple hydrogen bonds
Zn2+
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1 mM, 50% residual activity for extracellular isoform, not inhibitory for intracellular isoform
additional information
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reaction product D-glucose suppresses enzyme expression
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additional information
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insensitive to ATP, cyclic AMP or divalent cations
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additional information
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inhibitory potency of plant-derived inhibitors in different tissues on soluble and membranous enzymes, overview, mechanism of insect tocircumvent trehalase inhibition caused by glucosides, overview
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additional information
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inhibitory potency of plant-derived inhibitors in different tissues on soluble and membranous enzymes, overview, mechanism of insect tocircumvent trehalase inhibition caused by glucosides, overview
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additional information
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40 mM sodium fluoride and trehazolin have no inhibitory effect
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additional information
the enzyme activity is reduced under water-stress
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additional information
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the enzyme activity is reduced under water-stress
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additional information
Ca2+ has no significant inhibitory effect at 5 mM
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additional information
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Ca2+ has no significant inhibitory effect at 5 mM
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additional information
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sodium arsenate (45 mM), carbonyl cyanide m-chlorophenylhydrazone (0.14 mM), and sodium fluoride (28 mM) do not inhibit the activity
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additional information
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inhibitory potency of plant-derived inhibitors in different tissues on soluble and membranous enzymes, overview, mechanism of insect tocircumvent trehalase inhibition caused by glucosides, overview
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additional information
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no inhibition by 1,10-phenanthroline at 2 mM, and by gentiobiose
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additional information
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not inhibited by 1,3-bis((2R,3R,4R,5S)-3,4,5-trihydroxy-2-(hydroxymethyl)piperidin-1-yl)propane-1,3-dione
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additional information
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inhibitory potency of plant-derived inhibitors in different tissues on soluble and membranous enzymes, overview, mechanism of insect tocircumvent trehalase inhibition caused by glucosides, overview
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additional information
EDTA, polyaminopropyl biguanide, tetranitromethane, N-bromosuccinamide, gentiobiose (glucose-beta-1,6-glucose), methyl alpha-D-glucoside, 1,10-phenanthroline, and Tris (pH 6.0) do not inhibit the trehalase
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additional information
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EDTA, polyaminopropyl biguanide, tetranitromethane, N-bromosuccinamide, gentiobiose (glucose-beta-1,6-glucose), methyl alpha-D-glucoside, 1,10-phenanthroline, and Tris (pH 6.0) do not inhibit the trehalase
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