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Literature summary for 3.2.1.28 extracted from

  • Jorge, C.D.; Sampaio, M.M.; Hreggvidsson, G.O.; Kristjanson, J.K.; Santos, H.
    A highly thermostable trehalase from the thermophilic bacterium Rhodothermus marinus (2007), Extremophiles, 11, 115-122.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Rhodothermus marinus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 71% inhibition at 5 mM Rhodothermus marinus
D-glucose competitive inhibitor Rhodothermus marinus
EDTA moderate inhibitory level at 5 mM Rhodothermus marinus
Mg2+ moderate inhibitory level at 5 mM Rhodothermus marinus
Mn2+ moderate inhibitory level at 5 mM Rhodothermus marinus
additional information Ca2+ has no significant inhibitory effect at 5 mM Rhodothermus marinus
Zn2+ 95% inhibition at 5 mM Rhodothermus marinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
trehalose at 65°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) Rhodothermus marinus
0.27
-
trehalose at 88°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) Rhodothermus marinus

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Rhodothermus marinus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
SDS-PAGE Rhodothermus marinus
59270
-
calculated from amino acid sequence Rhodothermus marinus
77000
-
gel filtration Rhodothermus marinus

Organism

Organism UniProt Comment Textmining
Rhodothermus marinus A1XBT9
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Q Sepharose column chromatography Rhodothermus marinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose + H2O high specificity for trehalose Rhodothermus marinus D-glucose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 59000, SDS-PAGE Rhodothermus marinus

Synonyms

Synonyms Comment Organism
Alpha,alpha-trehalose glucohydrolase
-
Rhodothermus marinus
trehalase
-
Rhodothermus marinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
88
-
-
Rhodothermus marinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 90 the enzyme exhibits approximately a half-life of 6 h at 70°C, and of 2.5 h at 80°C, incubation of 10 min at 87°C results in 70% loss of the enzyme activity, and after 10 min at 90°C no residual activity is detected Rhodothermus marinus
80
-
the recombinant trehalase with a histidine tag at the N-terminus loses 50% of the activity after 10 min incubation at 80°C in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) Rhodothermus marinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Rhodothermus marinus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7
-
D-glucose at 65°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) Rhodothermus marinus