Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Rhodothermus marinus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 71% inhibition at 5 mM | Rhodothermus marinus | |
D-glucose | competitive inhibitor | Rhodothermus marinus | |
EDTA | moderate inhibitory level at 5 mM | Rhodothermus marinus | |
Mg2+ | moderate inhibitory level at 5 mM | Rhodothermus marinus | |
Mn2+ | moderate inhibitory level at 5 mM | Rhodothermus marinus | |
additional information | Ca2+ has no significant inhibitory effect at 5 mM | Rhodothermus marinus | |
Zn2+ | 95% inhibition at 5 mM | Rhodothermus marinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
trehalose | at 65°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) | Rhodothermus marinus | |
0.27 | - |
trehalose | at 88°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) | Rhodothermus marinus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Rhodothermus marinus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
SDS-PAGE | Rhodothermus marinus |
59270 | - |
calculated from amino acid sequence | Rhodothermus marinus |
77000 | - |
gel filtration | Rhodothermus marinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodothermus marinus | A1XBT9 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Q Sepharose column chromatography | Rhodothermus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trehalose + H2O | high specificity for trehalose | Rhodothermus marinus | D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 59000, SDS-PAGE | Rhodothermus marinus |
Synonyms | Comment | Organism |
---|---|---|
Alpha,alpha-trehalose glucohydrolase | - |
Rhodothermus marinus |
trehalase | - |
Rhodothermus marinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
88 | - |
- |
Rhodothermus marinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | the enzyme exhibits approximately a half-life of 6 h at 70°C, and of 2.5 h at 80°C, incubation of 10 min at 87°C results in 70% loss of the enzyme activity, and after 10 min at 90°C no residual activity is detected | Rhodothermus marinus |
80 | - |
the recombinant trehalase with a histidine tag at the N-terminus loses 50% of the activity after 10 min incubation at 80°C in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) | Rhodothermus marinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Rhodothermus marinus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
D-glucose | at 65°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) | Rhodothermus marinus |