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3.2.1.28: alpha,alpha-trehalase

This is an abbreviated version!
For detailed information about alpha,alpha-trehalase, go to the full flat file.

Word Map on EC 3.2.1.28

Reaction

alpha,alpha-trehalose
+
H2O
=
beta-D-glucose
 +
alpha-D-glucose

Synonyms

acid (non regulatory) trehalase, acid non-regulatory trehalase, acid trehalase, alkaline trehalase, alpha,alpha glucoside 1-glucohydrolase, alpha,alpha trehalase, alpha,alpha trehalose glucohydrolase, alpha,alpha'-trehalose 1-D-glucohydrolase, Alpha,alpha-trehalase, Alpha,alpha-trehalose glucohydrolase, alpha-glucoside-1-glucohydrolase, ATC1, Atc1p, Ath1, ATHA, ATM1, GH37 trehalase, glucose alpha-1,1-glucose hydrolase, HaTre-1, HaTreh-1, Hl-Tre, membrane-bound SfTre2, MSMEG 4528, neutral regulatory trehalase, neutral trehalase, NT-INV, Ntc1p, NTH1, Nth1p, Nth2p, NTHA, Ntp1p, Px_EclTre, Saci_1816, SeTre-1, SeTre-2, SfTre1, soluble P I type trehalase, soluble P II type trehalase, soluble trehalase, THA, Tre-2, TRE1, Tre37A, TreA, treB, TreF, Treh, Treh1, Treh2, trehalase, trehalase-1, trehalase-invertase, TreZ

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.28 alpha,alpha-trehalase

Specific Activity

Specific Activity on EC 3.2.1.28 - alpha,alpha-trehalase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.226
supernatant, at pH 6.0 and 30°C
0.227
-
cell extract, at 37°C
0.405
-
after 40fold purification, at pH 7.0 and 30°C
0.531
-
-
1.03
-
soluble trehalase P I after 86.9fold purification, in acetate buffer, pH 5.5, at 30°C
1.224
culture filtrate, in 20 mM MES buffer, pH 5.5, at 30°C
10.8
-
40°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition
100
-
40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition
101.3
recombinant enzyme, after purification, at 30°C
106.7
-
pH 5.5, 60°C, substrate: alpha,alpha-trehalose
11.2
-
40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition
11.5
-
40°C, pH 4.5, extracellular isoform, activity resistant to EDTA inhibition
113.4
-
40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition
117
-
after 232fold purification, at pH 5.5 and 55°C
12.2
membrane-bound isozyme
12.3
-
40°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition
12.4
-
60°C, pH 4.5, extracellular isoform, activity sensitive to EDTA inhibition
144.7
-
pH 5.4, 37°C, recombinant protein with N-terminal His-tag
154
-
60°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition
16.5
-
40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition
16.67
after 74fold purification, at pH 6.0 and 30°C
168
-
purified enzyme
1700
-
extracellular isoform, pH 6.0, 60°C
175.7
-
purified acid trehalase
1775
-
extracellular enzyme
18.4
-
40°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition
184
-
40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition
19.5
-
40°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition
195.5
-
40°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition
2.9
-
soluble trehalase P II after 245.7fold purification, in acetate buffer, pH 5.5, at 30°C
25.6
-
40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition
26.2
-
60°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition
27
-
40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition
274.8
-
60°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition
278
-
-
3.2
-
40°C, pH 4.5, extracellular isoform, activity resistant to EDTA inhibition
343
-
intestine
36.99
-
after 162fold purification, at 37°C
3700
-
intracellular isoform, pH 6.0, 65°C
389
-
40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition
39.4
soluble isozyme
4.8
-
40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition
42.2
-
40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition
46
-
40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition
48.6
-
40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition
49.6
-
60°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition
5.2
-
40°C, pH 4.5, extracellular isoform, activity sensitive to EDTA inhibition
50
-
free enzyme
53.4
-
solubilized enzyme
6.2
-
enzyme in periplasmic fraction, pH and temperature not specified in the publication
76.5
-
60°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition
76.8
-
60°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition
80 - 150
pH 7.0, 30°C
95.5
-
40°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition
additional information