General Stability | Organism |
---|---|
Ca2+ and Mn2+ protect from thermoinactivation | Mycothermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Mycothermus thermophilus | |
Al3+ | 1 mM AlCl3, complete inhibition | Mycothermus thermophilus | |
ATP | - |
Mycothermus thermophilus | |
Cu2+ | 1 mM CuSO4, complete inhibition of extracellular enzyme, 47% inhibition of intracellular enzyme | Mycothermus thermophilus | |
EDTA | 1 mM, 30% inhibition of extracellular enzyme, 54% inhibition of intracellular enzyme | Mycothermus thermophilus | |
Hg2+ | 1 mM HgCl2, complete inhibition | Mycothermus thermophilus | |
NH4Cl | 1 mM NH4Cl, 22% inhibition of extracellular enzyme, 27% inhibition of intracellular enzyme | Mycothermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.24 | - |
trehalose | intracellular enzyme | Mycothermus thermophilus | |
3.58 | - |
trehalose | extracellular enzyme | Mycothermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | intracellular and extracellular enzyme | Mycothermus thermophilus | - |
- |
intracellular | intracellular and extracellular enzyme | Mycothermus thermophilus | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Mycothermus thermophilus | |
Co2+ | slight stimulation | Mycothermus thermophilus | |
Mn2+ | activates | Mycothermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
5 * 82000, extracellular enzyme, SDS-PAGE | Mycothermus thermophilus |
85000 | - |
5 * 85000, intracellular enzyme, SDS-PAGE | Mycothermus thermophilus |
370000 | - |
extracellular enzyme, gel filtration | Mycothermus thermophilus |
398000 | - |
intracellular enzyme, gel filtration | Mycothermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycothermus thermophilus | - |
intracellular and extracellular threhalase | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | extracellular enzyme contains 81% carbohydrate, intracellular enzyme contains 51% carbohydrate | Mycothermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1775 | - |
extracellular enzyme | Mycothermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trehalose + H2O | absolute specificity | Mycothermus thermophilus | D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | 5 * 82000, extracellular enzyme, SDS-PAGE | Mycothermus thermophilus |
pentamer | 5 * 85000, intracellular enzyme, SDS-PAGE | Mycothermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
extracellular enzyme | Mycothermus thermophilus |
65 | - |
intracellular enzyme | Mycothermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
extracellular enzyme has a half-life of 3 min. Intracellular enzyme loses 20% of activity after 40 min when incubated without Ca2+, and is fully stable in presence of Ca2+ | Mycothermus thermophilus |
65 | - |
intracellular enzyme has a half-life of 4.5 min when incubated without Ca2+ | Mycothermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
extracellular and intracellular enzyme | Mycothermus thermophilus |