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0.06 - 72.8
alpha,alpha-trehalose
additional information
additional information
-
0.06
alpha,alpha-trehalose
mutant R222A/R287A, pH 7.0, 30°C
0.109
alpha,alpha-trehalose
-
pH 6.3, 37°C
0.16
alpha,alpha-trehalose
-
signal sequenceless TreA
0.2
alpha,alpha-trehalose
-
pH 4.5, 45°C
0.22
alpha,alpha-trehalose
mutant E520A, pH 7.0, 30°C
0.22
alpha,alpha-trehalose
mutant R287A, pH 7.0, 30°C
0.3
alpha,alpha-trehalose
wild-type, pH 7.0, 30°C
0.31
alpha,alpha-trehalose
-
periplasmic TreA
0.35
alpha,alpha-trehalose
-
pH 5.4, 37°C, recombinant protein with N-terminal His-tag
0.3793
alpha,alpha-trehalose
-
mutant enzyme Y227H/R442G, 50°C, pH 6.0
0.38
alpha,alpha-trehalose
-
-
0.43
alpha,alpha-trehalose
at pH 6.0 and 30°C
0.4317
alpha,alpha-trehalose
-
mutant enzyme R442G, 50°C, pH 6.5
0.44
alpha,alpha-trehalose
-
pH 5.5, 30°C
0.45
alpha,alpha-trehalose
mutant R169A, pH 7.0, 30°C
0.47
alpha,alpha-trehalose
-
pH 6.0, 30°C
0.47
alpha,alpha-trehalose
isozyme SfTre1
0.48
alpha,alpha-trehalose
-
pH 6.5, 30°C
0.5
alpha,alpha-trehalose
-
pH 5.5, 30°C, immobilized enzyme
0.52
alpha,alpha-trehalose
-
pH 7.5, 30°C
0.66
alpha,alpha-trehalose
-
-
0.7194
alpha,alpha-trehalose
-
mutant enzyme Y227H, 50°C, pH 6.5
0.86
alpha,alpha-trehalose
-
pH 5.5, 60°C
0.99
alpha,alpha-trehalose
-
wild type enzyme, 50°C, pH 6.5
1
alpha,alpha-trehalose
mutant D322A, pH 7.0, 30°C
1.11
alpha,alpha-trehalose
-
1.32
alpha,alpha-trehalose
-
pH 5.5, 30°C, soluble enzyme
1.34
alpha,alpha-trehalose
-
1.34
alpha,alpha-trehalose
pH and temperature not specified in the publication
1.37
alpha,alpha-trehalose
-
1.42
alpha,alpha-trehalose
-
in 50 mM phosphate buffer, at pH 5.5 and 37°C
1.47
alpha,alpha-trehalose
-
55°C, pH 5.0
1.5
alpha,alpha-trehalose
-
cytoplsmic wild-type treF
1.54
alpha,alpha-trehalose
-
1.6
alpha,alpha-trehalose
isozyme SfTre2
1.74
alpha,alpha-trehalose
-
1.77
alpha,alpha-trehalose
-
-
2.24
alpha,alpha-trehalose
-
intracellular isoform, pH 6.0, 65°C
3.09
alpha,alpha-trehalose
-
presence of Mn2+, intracellular isoform, pH 6.0, 65°C
3.33
alpha,alpha-trehalose
mutant R222A, pH 7.0, 30°C
3.58
alpha,alpha-trehalose
-
extracellular isoform, pH 6.0, 60°C
3.64
alpha,alpha-trehalose
-
at pH 5.5 and 55°C
6.43
alpha,alpha-trehalose
-
presence of Ca2+, intracellular isoform, pH 6.0, 65°C
6.6
alpha,alpha-trehalose
-
-
7.63
alpha,alpha-trehalose
-
presence of Mn2+, extracellular isoform, pH 6.0, 60°C
9.51
alpha,alpha-trehalose
-
presence of Ca2+, extracellular isoform, pH 6.0, 60°C
11.78
alpha,alpha-trehalose
-
-
20
alpha,alpha-trehalose
-
at 37°C
42
alpha,alpha-trehalose
pH 7.0, 30°C
72.8
alpha,alpha-trehalose
55°C, pH 7.4
72.8
alpha,alpha-trehalose
-
55°C, pH 7.4
0.0109
trehalose
-
in 50 mM acetate buffer, pH 4.6, at 30°C
0.031
trehalose
-
pH 3.3, 37°C
0.16
trehalose
at 65°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5)
0.27
trehalose
at 88°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5)
0.41
trehalose
at 37°C in sodium maleate buffer (75 mM, pH 5.5)
0.44
trehalose
-
soluble trehalase P II, inacetate buffer, pH 5.5, at 30°C
0.52
trehalose
recombinant enzyme, in 50 mM sodium phosphate buffer, pH 6.6, with 0.01% Triton X-100, at 35°C
0.6
trehalose
Lobosphaera sp.
-
-
0.63
trehalose
Thermochaetoides thermophila
-
presence of Ca2+
0.8
trehalose
-
extracellular enzyme
0.9
trehalose
-
cell wall-bound enzyme
1
trehalose
-
intracellular enzyme
1.4
trehalose
-
V-trehalase
1.56
trehalose
-
soluble trehalase P I, in acetate buffer, pH 5.5, at 30°C
1.9
trehalose
-
cytoplasmic enzyme
2.24
trehalose
-
intracellular enzyme
2.3
trehalose
in 20 mM MES buffer, pH 5.5, at 30°C
2.3
trehalose
native enzyme, in 20 mM MES, pH 6.0 for 10 min at 30°C
2.6
trehalose
recombinant enzyme, in 20 mM MES, pH 6.0 for 10 min at 30°C
3.15
trehalose
-
enzyme form kidney, intestine and amniotic fluid
3.3
trehalose
-
detergent-solubilized trehalase
3.58
trehalose
-
extracellular enzyme
3.6
trehalose
-
proteinase-treated trehalase
4.79
trehalose
-
in absence of trehalose-c
5.28
trehalose
-
in presence of trehalose-c
5.5
trehalose
-
phosphate buffer, pH 5.7
5.7
trehalose
-
C-trehalase
8
trehalose
-
actetate buffer, pH 5.0
10
trehalose
-
apparent value, at pH 7.1
11
trehalose
-
actetate buffer, pH 5.7
18
trehalose
pH 7.5, 37°C, recombinant enzyme
55
trehalose
-
trehalase from activated spores
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
trehalase from dormant spores shows a deviation from the Michaelis-Menten equation
-
additional information
additional information
-
Km of neutral and acid trehalase at different temperatures and pH values
-
additional information
additional information
-
Km of neutral and acid trehalase at different temperatures and pH values
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