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3.2.1.28: alpha,alpha-trehalase

This is an abbreviated version!
For detailed information about alpha,alpha-trehalase, go to the full flat file.

Word Map on EC 3.2.1.28

Reaction

alpha,alpha-trehalose
+
H2O
=
beta-D-glucose
 +
alpha-D-glucose

Synonyms

acid (non regulatory) trehalase, acid non-regulatory trehalase, acid trehalase, alkaline trehalase, alpha,alpha glucoside 1-glucohydrolase, alpha,alpha trehalase, alpha,alpha trehalose glucohydrolase, alpha,alpha'-trehalose 1-D-glucohydrolase, Alpha,alpha-trehalase, Alpha,alpha-trehalose glucohydrolase, alpha-glucoside-1-glucohydrolase, ATC1, Atc1p, Ath1, ATHA, ATM1, GH37 trehalase, glucose alpha-1,1-glucose hydrolase, HaTre-1, HaTreh-1, Hl-Tre, membrane-bound SfTre2, MSMEG 4528, neutral regulatory trehalase, neutral trehalase, NT-INV, Ntc1p, NTH1, Nth1p, Nth2p, NTHA, Ntp1p, Px_EclTre, Saci_1816, SeTre-1, SeTre-2, SfTre1, soluble P I type trehalase, soluble P II type trehalase, soluble trehalase, THA, Tre-2, TRE1, Tre37A, TreA, treB, TreF, Treh, Treh1, Treh2, trehalase, trehalase-1, trehalase-invertase, TreZ

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.28 alpha,alpha-trehalase

Cloned

Cloned on EC 3.2.1.28 - alpha,alpha-trehalase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of acid treahalase
-
DNA and amino acid sequence determination, analysis, and comparisons
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli strain BL21
expressed in Gluconobacter oxydans
-
expressed in Pichia pastoris strain GS115
expressed in Pichia pastoris strain KM71
expressed in yeast
-
expression in Escherichia coli
expression in Saccharomyces cerevisiae lacking the activites of endogenous trehalases Nth1, Nth2, or Ath1
-
expression in yeast
-
expression of GFP-tagged Ath1 N-terminal domain
-
gene ATC1, DNA and amino acid sequence determination and analysis, expression pattern, subcloning in Escherichia coli, in vitro transcription and translation of HA-tagged enzyme using an Escherichia coli-derived system
-
gene treH is organized in a gene cluster with the other two genes of the trehalose metabolism pathway, treZ and treY encoding malto-oligosyltrehalose trehalohydrolase and maltooligosyltrehalose synthase, DNA and amino acid sequence determination and analysis, overview. Expression of the N-terminally His-tagged active enzyme in Escherichia coli
genes NTH1 and ATH1
-
isozyme SfTre1, cDNA library preparation, DNA and amino acid sequence determination and analysis, phylogenetic tree and sequence comparisons, expression in Escherichia coli strain BL21(DE3)
isozyme SfTre2, cDNA library preparation, DNA and amino acid sequence determination and analysis, phylogenetic tree and sequence comparisons, expression in Escherichia coli strain BL21(DE3)
isozyme type 2
-
overexpression of the enzyme in strain Mph2 from plasmid pTRE11
-
soluble trehalase gene SeTre-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, developmental expression pattern of the two trehalase genes SeTre-1 and SeTre-2, overview
-