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3.2.1.28: alpha,alpha-trehalase

This is an abbreviated version!
For detailed information about alpha,alpha-trehalase, go to the full flat file.

Word Map on EC 3.2.1.28

Reaction

alpha,alpha-trehalose
+
H2O
=
beta-D-glucose
 +
alpha-D-glucose

Synonyms

acid (non regulatory) trehalase, acid non-regulatory trehalase, acid trehalase, alkaline trehalase, alpha,alpha glucoside 1-glucohydrolase, alpha,alpha trehalase, alpha,alpha trehalose glucohydrolase, alpha,alpha'-trehalose 1-D-glucohydrolase, Alpha,alpha-trehalase, Alpha,alpha-trehalose glucohydrolase, alpha-glucoside-1-glucohydrolase, ATC1, Atc1p, Ath1, ATHA, ATM1, GH37 trehalase, glucose alpha-1,1-glucose hydrolase, HaTre-1, HaTreh-1, Hl-Tre, membrane-bound SfTre2, MSMEG 4528, neutral regulatory trehalase, neutral trehalase, NT-INV, Ntc1p, NTH1, Nth1p, Nth2p, NTHA, Ntp1p, Px_EclTre, Saci_1816, SeTre-1, SeTre-2, SfTre1, soluble P I type trehalase, soluble P II type trehalase, soluble trehalase, THA, Tre-2, TRE1, Tre37A, TreA, treB, TreF, Treh, Treh1, Treh2, trehalase, trehalase-1, trehalase-invertase, TreZ

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.28 alpha,alpha-trehalase

Storage Stability

Storage Stability on EC 3.2.1.28 - alpha,alpha-trehalase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-12°C, stable for at least 2 months
-
-15°C, loses ca. 18% of activity after 3 months
-
-60°C, intestinal, renal and amniotic fluid enzyme retain full activity for at least 6 months
-
-80°C, 9 days, 15% loss of activity of enzyme form A1, 5% loss of activity of enzyme form A2, 35% loss of activity of enzyme form B1, 65% loss of activity of enzyme form B2
-
0-4°C, pH 4.5, 50% loss of activity after 1 month
-
0°C, 0.05 M maleate buffer, pH 6.5, stable for 1 month
-
4°-10°C, stable for at least 2-4 days
-
4°C, 0.02 M phosphate buffer, pH 6.2, the cryptic trehalase-c is completely stable in dilute aqueous solution and can be activated even after 6 months
-
4°C, pH 7.5, 20% glycerol, 20% loss of activity of trehalase-a after 5 months
-
4°C, stable for 3 months
-
immobilized crude recombinant enzyme on chitin shows no significant loss of activity being reused 10times and stored at 10°C, in 50 mM sodium maleate, pH 6.0, for 55 days
-