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Literature summary for 3.2.1.28 extracted from
- Characterization of a cytoplasmic trehalase of Escherichia coli (1996), J. Bacteriol., 178, 6250-6257.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 0.5 M KCl, activity is 2fold lower | Escherichia coli |  |
| Na+ | 0.5 M NaCl, activity is 2fold lower | Escherichia coli | |
| potassium glutamate | 0.5 M KCl, activity is 2fold lower | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
trehalose | cytoplasmic enzyme | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Escherichia coli | 5737 | - |
| periplasm | at high osmolarity a periplasmic trehalase is induced | Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | cytoplasmic enzyme has no metal dependency | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
1 * 64000, cytoplasmic enzyme, SDS-PAGE | Escherichia coli |
| 74000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose + H2O | Escherichia coli | pathway of trehalose utilization | D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from an overexpressing strain, cytoplasmic enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose + H2O | - |
Escherichia coli | D-glucose | - |
? | |
| trehalose + H2O | pathway of trehalose utilization | Escherichia coli | D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 64000, cytoplasmic enzyme, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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