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Literature summary for 3.2.1.28 extracted from
- Purification and properties of trehalase from rat intestinal mucosal cells (1977), J. Biochem., 81, 1041-1049.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 2 mM HgCl2, 61.7% inhibition | Rattus norvegicus |  |
| NEM | 2 mM, 8.4% inhibition | Rattus norvegicus | |
| PCMB | 2 mM, 30.1% inhibition | Rattus norvegicus | |
| phlorizin | 2 mM, 20% inhibition | Rattus norvegicus | |
| SDS | 1.0%, 80% inhibition | Rattus norvegicus | |
| Tris | 100 mM, 92% inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.5 | - |
trehalose | phosphate buffer, pH 5.7 | Rattus norvegicus | |
| 8 | - |
trehalose | actetate buffer, pH 5.0 | Rattus norvegicus | |
| 11 | - |
trehalose | actetate buffer, pH 5.7 | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2.5-5.0 mM CaCl2, slight stimulation | Rattus norvegicus | |
| Mg2+ | 2.5-5.0 mM MgCl2, slight stimulation | Rattus norvegicus | |
| Zn2+ | 2.5-5.0 mM ZnCl2, slight stimulation | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, SDS-PAGE | Rattus norvegicus |
| 240000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestine | microvillus membrane | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.4 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose + H2O | - |
Rattus norvegicus | D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.7 | - |
acetate buffer | Rattus norvegicus |
| 5.7 | - |
phosphate buffer or histidine buffer | Rattus norvegicus |
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