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Literature summary for 3.2.1.28 extracted from

  • Londesborough, J.; Varimo, K.
    Characterization of two trehalases in baker's yeast (1984), Biochem. J., 219, 511-518.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
MgATP2- C-trehalase can be activated by MgATP2- in presence of cAMP Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
acetate strong inhibition by acetic acid/acetate buffer, V-trehalase Saccharomyces cerevisiae
EDTA 1 mM, C-trehalase, complete inhibition Saccharomyces cerevisiae
K+ 100 mM KCl, 65% inhibition of C-trehalase Saccharomyces cerevisiae
MgCl2 50 mM, C-trehalase, complete inhibition Saccharomyces cerevisiae
ZnCl2 0.1 mM, C-trehalase, complete inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
trehalose V-trehalase Saccharomyces cerevisiae
5.7
-
trehalose C-trehalase Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ C-trehalase is absolutely dependent on Ca2+ or Mn2+. 4fold activation of C-trehalase at 2.5 mM CaCl2 Saccharomyces cerevisiae
K+ 30% activation by 100 mM KCl, V-trehalase Saccharomyces cerevisiae
Mn2+ 30% activation by 20-300 mM neutral salts such as KCl, NaNO3 and MnCl2, V-trehalase. C-trehalase is absolutely dependent on Ca2+ or Mn2+. 2fold activation of C-trehalase by MnCl2 Saccharomyces cerevisiae
Na+ 30% activation by 20-300 mM neutral salts such as KCl, NaNO3 and MnCl2, V-trehalase Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
C-trehalase, gel filtration Saccharomyces cerevisiae
215000
-
V-trehalase, gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
two enzyme activities, C-trehalase and V-trehalase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose + H2O
-
Saccharomyces cerevisiae D-glucose
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5 V-trehalase, at 40 mM trehalose Saccharomyces cerevisiae
6.7
-
at 40 mM trehalose, C-trehalase Saccharomyces cerevisiae