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3.2.1.28: alpha,alpha-trehalase

This is an abbreviated version!
For detailed information about alpha,alpha-trehalase, go to the full flat file.

Word Map on EC 3.2.1.28

Reaction

alpha,alpha-trehalose
+
H2O
=
beta-D-glucose
 +
alpha-D-glucose

Synonyms

acid (non regulatory) trehalase, acid non-regulatory trehalase, acid trehalase, alkaline trehalase, alpha,alpha glucoside 1-glucohydrolase, alpha,alpha trehalase, alpha,alpha trehalose glucohydrolase, alpha,alpha'-trehalose 1-D-glucohydrolase, Alpha,alpha-trehalase, Alpha,alpha-trehalose glucohydrolase, alpha-glucoside-1-glucohydrolase, ATC1, Atc1p, Ath1, ATHA, ATM1, GH37 trehalase, glucose alpha-1,1-glucose hydrolase, HaTre-1, HaTreh-1, Hl-Tre, membrane-bound SfTre2, MSMEG 4528, neutral regulatory trehalase, neutral trehalase, NT-INV, Ntc1p, NTH1, Nth1p, Nth2p, NTHA, Ntp1p, Px_EclTre, Saci_1816, SeTre-1, SeTre-2, SfTre1, soluble P I type trehalase, soluble P II type trehalase, soluble trehalase, THA, Tre-2, TRE1, Tre37A, TreA, treB, TreF, Treh, Treh1, Treh2, trehalase, trehalase-1, trehalase-invertase, TreZ

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.28 alpha,alpha-trehalase

Purification

Purification on EC 3.2.1.28 - alpha,alpha-trehalase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2896fold from midgut of larvae, by ammonium sulfate precipitation, 2 steps of hydrophobic interaction and ion exchange chromatography
-
4 enzyme form
-
acid trehalase 425fold from root nodules, by ammonium sulfate fractionation, ion exchange chromatography, gel filtration, and native PAGE
-
acid trehalase-sucrase aggregate
-
ammonium sulfate fractionation, phenyl-Sepharose DE-52 column chromatography and Sephacryl S-300 gel filtration
-
ammonium sulfate precipitation and DEAE-cellulose column chromatography
-
ammonium sulfate precipitation, Biosuite DEAE column chromatography, and gel filtration
-
ammonium sulfate precipitation, DEAE-cellulose column chromatography, CM Sepharose column chromatography, and Sephadex G-200 gel filtration
-
ammonium sulfate precipitation, DEAE-Sephadex-A50 column chromatography, and HPLC gel filtration on Protein Pak 300 SW
-
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Q Sepharose column chromatography
cryptic enzyme form
-
DEAE Sepharose Fast Flow column chromatography and Sephacryl S-100 gel filtration
Econo-Pac methyl hydrophobic interaction column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration
extracellular and intracellular enzyme
-
from an overexpressing strain, cytoplasmic enzyme
-
High Q Sepharose column chromatography, 25Q Sepharose column chromatography, and Sephacryl-200s HR gel filtration
IgG Sepharose column chromatography
-
native enzyme to homogeneity
-
Ni-NTA affinity column chromatography
nickel exchange chromatography
partial
preparation of periplasmic fraction of recombinant Escherichia coli
-
recombinant enzyme
recombinant His-tagged enzyme by His affinity chromatography to homogeneity
recombinant protein
-
Sephacryl S-300 gel filtration, DEAE-cellulofine column chromatography, and Con A-Sepharose column chromatography
-
stem enzyme 800fold by ion exchange chromatography, gel filtration, and other steps
-
with PEG 3350 (25% w/v) and bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane (Bis-Tris)/HCl buffer (0.1 m, pH 6.5)