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Literature summary for 3.2.1.28 extracted from

  • Silva, M.C.P.; Terra, W.R.; Ferreira, C.
    The role of carboxyl, guanidine and imidazole groups in catalysis by a midgut trehalase purified from an insect larvae (2004), Insect Biochem. Mol. Biol., 34, 1089-1099.
    View publication on PubMed

General Stability

General Stability Organism
Mn2+ stabilizes the enzyme during purification Spodoptera frugiperda

Inhibitors

Inhibitors Comment Organism Structure
amygdalin competitive inhibition Spodoptera frugiperda
mandelonitrile competitive inhibition Spodoptera frugiperda
methyl-alpha-glucoside weak competitive inhibition Spodoptera frugiperda
methyl-alpha-mannoside weak competitive inhibition Spodoptera frugiperda
additional information no inhibition by 1,10-phenanthroline at 2 mM, and by gentiobiose Spodoptera frugiperda
phlorizin hyperbolic uncompetitive, binds only to enzyme-substrate complex Spodoptera frugiperda
prunasin competitive inhibition Spodoptera frugiperda
Salicin competitive inhibition Spodoptera frugiperda
Tris competitive Spodoptera frugiperda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Spodoptera frugiperda
0.47
-
alpha,alpha-trehalose pH 6.0, 30°C Spodoptera frugiperda

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane about 10% Spodoptera frugiperda 16020
-
soluble about 90% Spodoptera frugiperda
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates, required Spodoptera frugiperda

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
x * 67000, SDS-PAGE Spodoptera frugiperda

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha,alpha-trehalose + H2O Spodoptera frugiperda
-
2 D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Spodoptera frugiperda
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2896fold from midgut of larvae, by ammonium sulfate precipitation, 2 steps of hydrophobic interaction and ion exchange chromatography Spodoptera frugiperda

Reaction

Reaction Comment Organism Reaction ID
alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose role of carboxyl, guanidine and imidazole groups in catalysis Spodoptera frugiperda

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
Spodoptera frugiperda
-
midgut larval Spodoptera frugiperda
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
168
-
purified enzyme Spodoptera frugiperda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O
-
Spodoptera frugiperda 2 D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 67000, SDS-PAGE Spodoptera frugiperda

Synonyms

Synonyms Comment Organism
trehalase
-
Spodoptera frugiperda

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Spodoptera frugiperda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Spodoptera frugiperda

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5 90% of maximal activity at pH 6.0, maximal activity at pH 7.0 Spodoptera frugiperda

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Spodoptera frugiperda
0.09
-
phlorizin pH 6.0, 30°C Spodoptera frugiperda
0.21
-
amygdalin pH 6.0, 30°C Spodoptera frugiperda
0.55
-
Tris pH 6.0, 30°C Spodoptera frugiperda
0.92
-
prunasin pH 6.0, 30°C Spodoptera frugiperda
1.14
-
mandelonitrile pH 6.0, 30°C Spodoptera frugiperda
6.2
-
methyl-alpha-mannoside pH 6.0, 30°C Spodoptera frugiperda
19
-
Salicin pH 6.0, 30°C Spodoptera frugiperda
89
-
methyl-alpha-glucoside pH 6.0, 30°C Spodoptera frugiperda