General Stability | Organism |
---|---|
Mn2+ stabilizes the enzyme during purification | Spodoptera frugiperda |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amygdalin | competitive inhibition | Spodoptera frugiperda | |
mandelonitrile | competitive inhibition | Spodoptera frugiperda | |
methyl-alpha-glucoside | weak competitive inhibition | Spodoptera frugiperda | |
methyl-alpha-mannoside | weak competitive inhibition | Spodoptera frugiperda | |
additional information | no inhibition by 1,10-phenanthroline at 2 mM, and by gentiobiose | Spodoptera frugiperda | |
phlorizin | hyperbolic uncompetitive, binds only to enzyme-substrate complex | Spodoptera frugiperda | |
prunasin | competitive inhibition | Spodoptera frugiperda | |
Salicin | competitive inhibition | Spodoptera frugiperda | |
Tris | competitive | Spodoptera frugiperda |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Spodoptera frugiperda | |
0.47 | - |
alpha,alpha-trehalose | pH 6.0, 30°C | Spodoptera frugiperda |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | about 10% | Spodoptera frugiperda | 16020 | - |
soluble | about 90% | Spodoptera frugiperda | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates, required | Spodoptera frugiperda |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, SDS-PAGE | Spodoptera frugiperda |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | Spodoptera frugiperda | - |
2 D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spodoptera frugiperda | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2896fold from midgut of larvae, by ammonium sulfate precipitation, 2 steps of hydrophobic interaction and ion exchange chromatography | Spodoptera frugiperda |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose | role of carboxyl, guanidine and imidazole groups in catalysis | Spodoptera frugiperda |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Spodoptera frugiperda | - |
midgut | larval | Spodoptera frugiperda | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
168 | - |
purified enzyme | Spodoptera frugiperda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | - |
Spodoptera frugiperda | 2 D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, SDS-PAGE | Spodoptera frugiperda |
Synonyms | Comment | Organism |
---|---|---|
trehalase | - |
Spodoptera frugiperda |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Spodoptera frugiperda |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Spodoptera frugiperda |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8.5 | 90% of maximal activity at pH 6.0, maximal activity at pH 7.0 | Spodoptera frugiperda |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Spodoptera frugiperda | |
0.09 | - |
phlorizin | pH 6.0, 30°C | Spodoptera frugiperda | |
0.21 | - |
amygdalin | pH 6.0, 30°C | Spodoptera frugiperda | |
0.55 | - |
Tris | pH 6.0, 30°C | Spodoptera frugiperda | |
0.92 | - |
prunasin | pH 6.0, 30°C | Spodoptera frugiperda | |
1.14 | - |
mandelonitrile | pH 6.0, 30°C | Spodoptera frugiperda | |
6.2 | - |
methyl-alpha-mannoside | pH 6.0, 30°C | Spodoptera frugiperda | |
19 | - |
Salicin | pH 6.0, 30°C | Spodoptera frugiperda | |
89 | - |
methyl-alpha-glucoside | pH 6.0, 30°C | Spodoptera frugiperda |