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Literature summary for 3.2.1.28 extracted from

  • Lopez, M.F.; Torrey, J.G.
    Purification and properties of trehalase in Frankia ArI3 (1985), Arch. Microbiol., 143, 209-215.
No PubMed abstract available
Search for more literature for 3.2.1.28

Inhibitors

Inhibitors Comment Organism Structure
glucose
-
 Frankia sp.
mannitol partial Frankia sp.
mannose
-
 Frankia sp.
sucrose
-
 Frankia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2
-
 trehalose
-
 Frankia sp.
4.7
-
 trehalose
-
 Frankia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trehalose + H2O Frankia sp. trehalase activity in crude extracts increases over time when cells are induced to fix nitrogen D-glucose
-
 ?
trehalose + H2O Frankia sp. ArI3 trehalase activity in crude extracts increases over time when cells are induced to fix nitrogen D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Frankia sp.
-
-
-
Frankia sp. ArI3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Frankia sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture activity in nitrogen-fixing pure culture is about 4times greater than in non-fixing cultures Frankia sp.
-
nodule vesicle clusters isolated from Alnus rubra nitrogen-fixing nodules infected with ArI3. Activity is 6-7 times greater than in nitrogen-fixing cultures and 26-33 times greater than in non-fixing cultures Frankia sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Frankia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose + H2O
-
 Frankia sp. D-glucose
-
 ?
trehalose + H2O trehalase activity in crude extracts increases over time when cells are induced to fix nitrogen Frankia sp. D-glucose
-
 ?
trehalose + H2O
-
 Frankia sp. ArI3 D-glucose
-
 ?
trehalose + H2O trehalase activity in crude extracts increases over time when cells are induced to fix nitrogen Frankia sp. ArI3 D-glucose
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.3
-
 Frankia sp.

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 pH 3.5: about 75% of maximal activity, pH 6.0: about 80% of maximal activity Frankia sp.