Application | Comment | Organism |
---|---|---|
synthesis | expression as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system. Only N-terminally tagged trehalase shows a high activity | Bombyx mori |
Cloned (Comment) | Organism |
---|---|
- |
Bombyx mori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
validoxylamine A | complete inhibition | Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
alpha,alpha-trehalose | pH 5.4, 37°C, recombinant protein with N-terminal His-tag | Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
x * 68000, recombinant enzyme with His-tag | Bombyx mori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | recombinant protein with N-terminal His-tag contains O-linked oligosaccharide chains having Galbeta1-3GalNAc-structure without terminal sialic acids | Bombyx mori |
Purification (Comment) | Organism |
---|---|
recombinant protein | Bombyx mori |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
144.7 | - |
pH 5.4, 37°C, recombinant protein with N-terminal His-tag | Bombyx mori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | - |
Bombyx mori | 2 D-glucose | - |
? | |
additional information | no substrate: maltose, isomaltose, laminaribiose, cellobiose, sucrose, lactose | Bombyx mori | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, recombinant enzyme with His-tag | Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant protein with N-terminal His-tag | Bombyx mori |