Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.28 extracted from

  • Ujita, M.; Yamanaka, M.; Maeno, Y.; Yoshida, K.; Ohshio, W.; Ueno, Y.; Banno, Y.; Fujii, H.; Okumura, H.
    Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures (2011), J. Biosci. Bioeng., 111, 22-25.
    View publication on PubMed

Application

Application Comment Organism
synthesis expression as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system. Only N-terminally tagged trehalase shows a high activity Bombyx mori

Cloned(Commentary)

Cloned (Comment) Organism
-
Bombyx mori

Inhibitors

Inhibitors Comment Organism Structure
validoxylamine A complete inhibition Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
alpha,alpha-trehalose pH 5.4, 37°C, recombinant protein with N-terminal His-tag Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
x * 68000, recombinant enzyme with His-tag Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant protein with N-terminal His-tag contains O-linked oligosaccharide chains having Galbeta1-3GalNAc-structure without terminal sialic acids Bombyx mori

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Bombyx mori

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
144.7
-
pH 5.4, 37°C, recombinant protein with N-terminal His-tag Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O
-
Bombyx mori 2 D-glucose
-
?
additional information no substrate: maltose, isomaltose, laminaribiose, cellobiose, sucrose, lactose Bombyx mori ?
-
?

Subunits

Subunits Comment Organism
? x * 68000, recombinant enzyme with His-tag Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
recombinant protein with N-terminal His-tag Bombyx mori