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3.2.1.15: endo-polygalacturonase

This is an abbreviated version!
For detailed information about endo-polygalacturonase, go to the full flat file.

Word Map on EC 3.2.1.15

Reaction

(1,4-alpha-D-galacturonosyl)n+m
+
H2O
=
(1,4-alpha-D-galacturonosyl)n
+
(1,4-alpha-D-galacturonosyl)m

Synonyms

ADPG1, ADPG2, Aj-PGase, alkaline endo-PG, ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1, At1g48100, BcPeh28A, BPN3, CluPG1, D-galacturonase, endo PG, endo-D-galacturonanase, endo-D-galacturonase, endo-PG, endo-PG I, endo-pgaA, endo-polygalacturonase, endo-polygalacturonase A, endo-polygalacturonase C, endo-polygalacturonase I, endo-polygalacturonase II, endo-polygalacturonase-3, endo-TePG28b, endogalacturonase, endopectinase, endoPG, EndoPG I, endoPG II, endopolygalacturonase, endopolygalacturonase I, endopolygalacturonases, endopolygalacturonate lyase, EnPG28A, EPG, Epg1-2p, EPG4, Exo-PG, exo-polygalacturonase, FgPG, FpPG, liquifying polygalacturonase, LLP-A1.1 protein, LLP-PG, Magnaporthe oryzae density dependent germination regulator, male fertility 9, MDG1, MF9, More, MpPG2, non-acidic polygalacturonase, OGH, oligogalacturonate hydrolase, P1/P3, P2C, pectate hydrolase, pectic depolymerase, pectic hydrolase, pectin depolymerase, pectin hydrolase, pectin polygalacturonase, pectinase, pectinase SS, pectolase, pectozyme, Peh28A, PehA, PehB, PG, PG II, PG-2A, PG-3, PG1, PG2, PG28A, PG3, PG4, PG5, PG6, PG63, PG8fn, PGA, PGase, PGase SM, PGC, PGI, PGII, PGIP, PGL, Pgu1, phylendonase, poly [1, 4-alpha-D-galacturonide] glycano-hydrolase, poly-alpha-1,4-galacturonide glycanohydrolase, poly-[1,4-alpha-D-galacturonide] glycanohydrolase, polygalacturonase 1, polygalacturonase I, polygalacturonase II, polygalacturonase inhibiting protein 1, polygalacturonase p36, polygalacturonase p40, polygalacturonase-3, polygalacturonase-inhibitor-like, QRT2, QUARTET2, remanase, RPG1, ScPgu1, SpPgu1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.15 endo-polygalacturonase

Molecular Weight

Molecular Weight on EC 3.2.1.15 - endo-polygalacturonase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
gel filtration, PG1
105000
-
gel filtration
106000
-
native PAGE
18000
-
gel filtration
19000
-
1 * 19000, SDS-PAGE
199500
-
polygalacturonase I, gel filtration
20000
-
gel filtration
21000
-
1 * 62000 + 1 * 21000, SDS-PAGE
24000
-
x * 24000, SDS-PAGE
29000
-
x * 29000, SDS-PAGE
29300
-
x * 29300, SDS-PAGE
30000
30200
-
gel filtration
30600
31000
32000
33000
Saccharomyces fragilis
-
gel filtration
34000
34100
-
polygalacturonase I, gel filtration
34600
-
1 * 34600, polygalacturonase I, SDS-PAGE
34632
-
x * 34632, ESI-MS
34751
-
x * 34751, calculated from sequence
35000
35400
x * 35400, about, sequence calculation, x * 36900, about, mass spectrometry
35500
36000
36200
-
MALDI-TOF MS, deglycosylated PGA
36430 - 36750
-
MALDI-TOF-MS analysis
36600
-
gel filtration
36900
x * 35400, about, sequence calculation, x * 36900, about, mass spectrometry
37000
37230
-
MALDI-TOF MS, recombinant PGA
37500
38000
38530
x * 38530, about, sequence calculation
38700
-
x * 38700, mass spectrometry
39500
-
PG1
39600
-
MALDI-TOF MS
40000
40064
-
x * 40064, calculation from nucleotide sequence
40279
-
x * 40279, calculation from nucleotide sequence
40900
deduced for the mature protein, calculated from the nucleotide sequence of the full length PG1 cDNA
41000
41700
-
x * 41700, SDS-PAGE
42000
43000
43100
-
gel filtration
43500
-
x * 43500, SDS-PAGE
44000
45000
45700
-
polygalacturonase II, gel filtration
46000
47000
1 * 47000, purified enzyme, SDS-PAGE
48000
-
gel filtration
50000
50788
x * 50788, calculation from nucleotide sequence
51400
-
equilibrium sedimentation
51600
-
gel filtration
52000
-
x * 52000, SDS-PAGE
56200
-
polygalacturonase II, gel filtration
56500
-
1 * 56500, SDS-PAGE
56800
-
1 * 56800, polygalacturonase II, SDS-PAGE
57000
-
gel filtration
58000
-
x * 58000, SDS-PAGE
60000
60000 - 70000
-
gel filtration
62000
-
1 * 62000 + 1 * 21000, SDS-PAGE
63000
-
PG2, gel filtration
63300
-
x * 63300, SDS-PAGE, x * 43000, SDS-PAGE, x * 30000, SDS-PAGE
6500
-
SDS-PAGE
65000
-
x * 65000, polygalacturonase II, SDS-PAGE
66000
69000
-
1 * 34000 + 1 * 69000, SDS-PAGE
84000
-
polygalacturonase I, gel filtration
85000
-
gel filtration
90000
-
gel filtration
additional information
-
the main band has a MW of 75900 Da and the acid band has a MW of 32200 Da as determined by gel filtration