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Literature summary for 3.2.1.15 extracted from
- Expression, purification, and crystallization of endopolygalacturonase from a pathogenic fungus, Stereum purpureum, in Escherichia coli (2005), Protein Expr. Purif., 44, 130-135.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain Origami (DE3) provides a soluble and active enzyme with proper disulfide bond formation, the enzyme expressed in BL21 is localized in inclusion bodies | Chondrostereum purpureum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapor diffusion method at 20°C, pH 5.0 | Chondrostereum purpureum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | expression in Escherichia coli strain Origami (DE3) provides a soluble and active enzyme with proper disulfide bond formation, the enzyme expressed in BL21 is localized in inclusion bodies | Chondrostereum purpureum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34632 | - |
x * 34632, ESI-MS | Chondrostereum purpureum |
| 34751 | - |
x * 34751, calculated from sequence | Chondrostereum purpureum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chondrostereum purpureum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chondrostereum purpureum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 442 | - |
- |
Chondrostereum purpureum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid + H2O | random hydrolysis of the alpha-1,4 glycosidic linkages | Chondrostereum purpureum | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34632, ESI-MS | Chondrostereum purpureum |
| ? | x * 34751, calculated from sequence | Chondrostereum purpureum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EndoPG I | - |
Chondrostereum purpureum |
| endopolygalacturonase | - |
Chondrostereum purpureum |
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Release 2023.1 (January 2023)
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