Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 18% inhibition | Thermoascus aurantiacus | |
EDTA | 27% inhibition | Thermoascus aurantiacus | |
Hg2+ | complete inhibition | Thermoascus aurantiacus | |
Mg2+ | 24% inhibition | Thermoascus aurantiacus | |
Mn2+ | 77% inhibition | Thermoascus aurantiacus | |
Zn2+ | 59% inhibition | Thermoascus aurantiacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 1.58 mg/ml with polygalacturonan at pH 5.5, 60°C | Thermoascus aurantiacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by K+ and poor effects by Ca2+ and Fe3+ at 2 mM | Thermoascus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29300 | - |
x * 29300, SDS-PAGE | Thermoascus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | Thermoascus aurantiacus | citrus pectin with 26% and 92% degree of esterification, and apple pectin | ? | - |
? | |
pectin + H2O | Thermoascus aurantiacus CBMAI-756 | citrus pectin with 26% and 92% degree of esterification, and apple pectin | ? | - |
? | |
polygalacturonan + H2O | Thermoascus aurantiacus | - |
? | - |
? | |
polygalacturonan + H2O | Thermoascus aurantiacus CBMAI-756 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
- |
- |
Thermoascus aurantiacus CBMAI-756 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 89.2fold by gel filtration and cation exchange chromatography to homogeneity | Thermoascus aurantiacus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | on solid-state fermentation carried out in culture media containing sugar cane bagasse and orange bagasse in proportions of 30% and 70% w/w at 45°C for 4 days | Thermoascus aurantiacus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
5351.50 U/mg, one unit of enzyme activity is defined as the amount of enzyme that reduced the initial viscosity by 50% per minute, pH 5.5, 60°C, substrate pectin, purified enzyme | Thermoascus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | polygalacturonase is an endo/exoenzyme, cf. EC 3.2.1.67 | Thermoascus aurantiacus | ? | - |
? | |
additional information | polygalacturonase is an endo/exoenzyme, cf. EC 3.2.1.67 | Thermoascus aurantiacus CBMAI-756 | ? | - |
? | |
pectin + H2O | citrus pectin with 26% and 92% degree of esterification, and apple pectin | Thermoascus aurantiacus | ? | - |
? | |
pectin + H2O | citrus pectin with 26% and 92% degree of esterification, and apple pectin | Thermoascus aurantiacus CBMAI-756 | ? | - |
? | |
polygalacturonan + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
polygalacturonan + H2O | - |
Thermoascus aurantiacus CBMAI-756 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29300, SDS-PAGE | Thermoascus aurantiacus |
Synonyms | Comment | Organism |
---|---|---|
endo-PG | - |
Thermoascus aurantiacus |
endo-polygalacturonase | - |
Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | - |
Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | purified enzyme, completely stable for 1 h at 50°C. At 55°C and 60°C, the activity decreases by 55% and 90%, respectively. After 50 min inactivation at 60°C | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.5 | - |
Thermoascus aurantiacus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | the enzyme shows the highest activity between pH 4.5 and pH 5.5, and more than 80% between pH 5.0 and pH 6.5. At pH 3.0-4.5, the enzyme shows about 73% activity, at pH 10.0 44% activity | Thermoascus aurantiacus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermoascus aurantiacus | isoelectric focusing | - |
6.6 |