Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.15 extracted from

  • Martins, E. da S.; Leite, R.S.; da Silva, R.; Gomes, E.
    Purification and properties of polygalacturonase produced by thermophilic fungus Thermoascus aurantiacus CBMAI-756 on solid-state fermentation (2013), Enzyme Res., 2013, 438645.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 18% inhibition Thermoascus aurantiacus
EDTA 27% inhibition Thermoascus aurantiacus
Hg2+ complete inhibition Thermoascus aurantiacus
Mg2+ 24% inhibition Thermoascus aurantiacus
Mn2+ 77% inhibition Thermoascus aurantiacus
Zn2+ 59% inhibition Thermoascus aurantiacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 1.58 mg/ml with polygalacturonan at pH 5.5, 60°C Thermoascus aurantiacus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect by K+ and poor effects by Ca2+ and Fe3+ at 2 mM Thermoascus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29300
-
x * 29300, SDS-PAGE Thermoascus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pectin + H2O Thermoascus aurantiacus citrus pectin with 26% and 92% degree of esterification, and apple pectin ?
-
?
pectin + H2O Thermoascus aurantiacus CBMAI-756 citrus pectin with 26% and 92% degree of esterification, and apple pectin ?
-
?
polygalacturonan + H2O Thermoascus aurantiacus
-
?
-
?
polygalacturonan + H2O Thermoascus aurantiacus CBMAI-756
-
?
-
?

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus
-
-
-
Thermoascus aurantiacus CBMAI-756
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 89.2fold by gel filtration and cation exchange chromatography to homogeneity Thermoascus aurantiacus

Source Tissue

Source Tissue Comment Organism Textmining
additional information on solid-state fermentation carried out in culture media containing sugar cane bagasse and orange bagasse in proportions of 30% and 70% w/w at 45°C for 4 days Thermoascus aurantiacus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
5351.50 U/mg, one unit of enzyme activity is defined as the amount of enzyme that reduced the initial viscosity by 50% per minute, pH 5.5, 60°C, substrate pectin, purified enzyme Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information polygalacturonase is an endo/exoenzyme, cf. EC 3.2.1.67 Thermoascus aurantiacus ?
-
?
additional information polygalacturonase is an endo/exoenzyme, cf. EC 3.2.1.67 Thermoascus aurantiacus CBMAI-756 ?
-
?
pectin + H2O citrus pectin with 26% and 92% degree of esterification, and apple pectin Thermoascus aurantiacus ?
-
?
pectin + H2O citrus pectin with 26% and 92% degree of esterification, and apple pectin Thermoascus aurantiacus CBMAI-756 ?
-
?
polygalacturonan + H2O
-
Thermoascus aurantiacus ?
-
?
polygalacturonan + H2O
-
Thermoascus aurantiacus CBMAI-756 ?
-
?

Subunits

Subunits Comment Organism
? x * 29300, SDS-PAGE Thermoascus aurantiacus

Synonyms

Synonyms Comment Organism
endo-PG
-
Thermoascus aurantiacus
endo-polygalacturonase
-
Thermoascus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65
-
Thermoascus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 purified enzyme, completely stable for 1 h at 50°C. At 55°C and 60°C, the activity decreases by 55% and 90%, respectively. After 50 min inactivation at 60°C Thermoascus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5
-
Thermoascus aurantiacus

pH Range

pH Minimum pH Maximum Comment Organism
3 10 the enzyme shows the highest activity between pH 4.5 and pH 5.5, and more than 80% between pH 5.0 and pH 6.5. At pH 3.0-4.5, the enzyme shows about 73% activity, at pH 10.0 44% activity Thermoascus aurantiacus

pI Value

Organism Comment pI Value Maximum pI Value
Thermoascus aurantiacus isoelectric focusing
-
6.6