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Literature summary for 3.2.1.15 extracted from
- Isolation and properties of pectinases from the fungus Aspergillus japonicus (2003), Biochemistry (Moscow), 68, 559-569.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km value for polygalacturonate is 1.03 g/l with polygalacturonase I. Km value for polygalacturonate is 1.03 g/L with polygalacturonase II | Aspergillus japonicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | maximal activity at 0.1-0.2 M, polygalacturonase I and II | Aspergillus japonicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, polygalacturonase I, SDS-PAGE | Aspergillus japonicus |
| 65000 | - |
x * 65000, polygalacturonase II, SDS-PAGE | Aspergillus japonicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus japonicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus japonicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate + H2O | - |
Aspergillus japonicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, polygalacturonase I, SDS-PAGE | Aspergillus japonicus |
| ? | x * 65000, polygalacturonase II, SDS-PAGE | Aspergillus japonicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-polygalacturonase I | - |
Aspergillus japonicus |
| endo-polygalacturonase II | - |
Aspergillus japonicus |
| polygalacturonase I | - |
Aspergillus japonicus |
| polygalacturonase II | - |
Aspergillus japonicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
about 40% inactivation of polygalacturonase I after 60 min, about 10% inactivation of polygalacturonase II after 60 min | Aspergillus japonicus |
| 40 | - |
about 55% inactivation of polygalacturonase I after 60 min, about 50% inactivation of polygalacturonase II after 60 min | Aspergillus japonicus |
| 50 | - |
complete inactivation of polygalacturonase I after 10 min, complete inactivation of polygalacturonase II after 40 min | Aspergillus japonicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26 | - |
Polygalacturonate | polygalacturonase II | Aspergillus japonicus | |
| 90 | - |
Polygalacturonate | polygalacturonase I | Aspergillus japonicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
polygalacturonase II | Aspergillus japonicus |
| 5 | - |
polygalacturonase I | Aspergillus japonicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 5.5 | pH 3.5: about 50% of maximal activity, pH 5.5: about 60% of maximal activity, polygalacturonase II | Aspergillus japonicus |
| 4.5 | 5.5 | pH 4.5: about 50% of maximal activity, pH 5.5: about 40% of maximal activity, polygalacturonase I | Aspergillus japonicus |
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