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3.2.1.15: endo-polygalacturonase

This is an abbreviated version!
For detailed information about endo-polygalacturonase, go to the full flat file.

Word Map on EC 3.2.1.15

Reaction

(1,4-alpha-D-galacturonosyl)n+m
+
H2O
=
(1,4-alpha-D-galacturonosyl)n
 +
(1,4-alpha-D-galacturonosyl)m

Synonyms

ADPG1, ADPG2, Aj-PGase, alkaline endo-PG, ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1, At1g48100, BcPeh28A, BPN3, CluPG1, D-galacturonase, endo PG, endo-D-galacturonanase, endo-D-galacturonase, endo-PG, endo-PG I, endo-pgaA, endo-polygalacturonase, endo-polygalacturonase A, endo-polygalacturonase C, endo-polygalacturonase I, endo-polygalacturonase II, endo-polygalacturonase-3, endo-TePG28b, endogalacturonase, endopectinase, endoPG, EndoPG I, endoPG II, endopolygalacturonase, endopolygalacturonase I, endopolygalacturonases, endopolygalacturonate lyase, EnPG28A, EPG, Epg1-2p, EPG4, Exo-PG, exo-polygalacturonase, FgPG, FpPG, liquifying polygalacturonase, LLP-A1.1 protein, LLP-PG, Magnaporthe oryzae density dependent germination regulator, male fertility 9, MDG1, MF9, More, MpPG2, non-acidic polygalacturonase, OGH, oligogalacturonate hydrolase, P1/P3, P2C, pectate hydrolase, pectic depolymerase, pectic hydrolase, pectin depolymerase, pectin hydrolase, pectin polygalacturonase, pectinase, pectinase SS, pectolase, pectozyme, Peh28A, PehA, PehB, PG, PG II, PG-2A, PG-3, PG1, PG2, PG28A, PG3, PG4, PG5, PG6, PG63, PG8fn, PGA, PGase, PGase SM, PGC, PGI, PGII, PGIP, PGL, Pgu1, phylendonase, poly [1, 4-alpha-D-galacturonide] glycano-hydrolase, poly-alpha-1,4-galacturonide glycanohydrolase, poly-[1,4-alpha-D-galacturonide] glycanohydrolase, polygalacturonase 1, polygalacturonase I, polygalacturonase II, polygalacturonase inhibiting protein 1, polygalacturonase p36, polygalacturonase p40, polygalacturonase-3, polygalacturonase-inhibitor-like, QRT2, QUARTET2, remanase, RPG1, ScPgu1, SpPgu1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.15 endo-polygalacturonase

Specific Activity

Specific Activity on EC 3.2.1.15 - endo-polygalacturonase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00199
-
in crude extract from unripe fruit
0.00664
-
in cride extract from ripe fruit
0.0319
-
crude extract, in 100 mM sodium acetate, pH 5.5, containing 0.4% polygalacturonic acid, at 20°C
0.102
-
after purification, in 100 mM sodium acetate, pH 5.5, containing 0.4% polygalacturonic acid, at 20°C
1.28
-
with apple pomace as the carbon source
100.56
-
crude enzyme, at pH 9.1 and 60°C
103.5
54.4fold purified
112600
-
207fold purified enzyme
13.7
-
culture supernatant of EndoPG I
130
Saccharomyces fragilis
-
isoenzyme II
1328
-
85fold purified enzyme
134.3
Saccharomyces fragilis
-
isoenzyme III
13790
-
purified enzyme, pH 9.0, 60°C
14.5
-
grown on tomato pomace, third day of culture
14.75
-
-
145
Saccharomyces fragilis
-
isoenzyme I
147
-
enzyme 9.97fold purified, in the presence of 2 mM imidazole
148
-
polygalacturonase 2
15
-
PG5 in fermentation broth
1506
-
100fold purified PG5
16.1
-
crude extract
16.87
-
3.02fold purified enzyme
178.2
-
enzyme 12.34fold purified, in the presence of 4 mM imidazole
189.8
P19805
purified enzyme, pH 5.0, 50°C
2.33
crude extract, at pH 4.5 and 30°C
2.713
-
At1g48100
21
-
culture filtrate
21.1
5.8fold purified enzyme
2216.4
-
after 22.04fold purification, at pH 9.1 and 60°C
2350
-
enzyme purified from culture broth by centrifugation, with 10 kDa ultrafiltration membrane, 450 nm microfiltration membrane and 50 kDa ultrafiltration membrane, 1.98fold
24.2
6.9fold purified
246
-
partially purified enzyme, pH 4.5, 40°C
248
-
grown on pectin as a sole carbon source
258
-
partially purified enzyme, pH 5.0, 30°C
276
-
13fold purified enzyme
28120
purified recombinant enzyme, pH 6.0, 45°C
3.01
-
with galacturonic acid as the carbon source
3030
-
enzyme purified from culture broth with 500 kDa ultrafiltration membrane
307
-
22.4fold purified recombinant EndoPG I with four sugar chains
3130
-
enzyme purified from culture broth with 100 kDa ultrafiltration membrane
322.9
-
purified enzyme, pH 5.0, 40°C
3230
-
enzyme purified from culture broth with 25 kDa ultrafiltration membrane
3530
-
enzyme purified from culture broth with 50 kDa ultrafiltration membrane
4.81
-
with orange waste as the carbon source
408
-
29.8fold purified recombinant EndoPG I with one sugar chain
418
-
30.5fold purified recombinant EndoPG I with three sugar chains
43.9
-
enzyme form E2
435
after 187fold purification, at pH 4.5 and 30°C
4380
-
enzyme purified from culture broth with 200 nm microfiltration membrane
4440
-
enzyme purified from culture broth with 450 nm microfiltration membrane
447
-
32.6fold purified recombinant EndoPG I with two sugar chains
4585
-
306fold purified PG5 fraction PGII
5.59
-
crude extract
5000
-
13.7fold purified enzyme
525.9
-
purified enzyme, pH 5.0, 55°C, substrate pectin
54.3
-
determined as micromol/ml/mg protein, purified enzyme, pH 4.8, 45°C, substrate polygalacturonic acid
542
-
culture filtrate
5590
-
enzyme purified from culture broth with 450 nm microfiltration membrane and 50 kDa ultrafiltration membrane, 4.69fold
6.17
Tetracoccosporium sp.
-
-
6120
-
enzyme purified from culture broth with 10 kDa ultrafiltration membrane, 450 nm microfiltration membrane and 50 kDa ultrafiltration membrane, 5.14fold
7.24
-
with polygalacturonic acid as the carbon source
7.35
-
with citrus pectin as the carbon source
7.53
-
purified enzyme, pH 3.5, 40°C
80.8
-
enzyme form E1
83.06
-
partially purified enzyme, pH 4.5, 50°C
additional information