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Literature summary for 3.2.1.15 extracted from
- Production of alkaline enzymes by alkalophilic microorganisms (1972), Agric. Biol. Chem., 36, 285-293.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ stabilizes | Bacillus sp. (in: Bacteria) |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM, complete inactivation | Bacillus sp. (in: Bacteria) |  |
| NaCl | 0.3 M, complete inactivation | Bacillus sp. (in: Bacteria) | |
| PCMB | 0.1 M, complete inactivation | Bacillus sp. (in: Bacteria) | |
| Urea | 8 M, complete inactivation | Bacillus sp. (in: Bacteria) | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | maximal activation at 1 mM | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | 70000 | gel filtration | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
No. P-4-N | - |
| Bacillus sp. (in: Bacteria) No. P-4-N | - |
No. P-4-N | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectic acid + H2O | - |
Bacillus sp. (in: Bacteria) | galacturonic acid oligomers | - |
? | |
| pectic acid + H2O | - |
Bacillus sp. (in: Bacteria) No. P-4-N | galacturonic acid oligomers | - |
? | |
| polygalacturonate + H2O | - |
Bacillus sp. (in: Bacteria) | oligogalacturonates | liberation of digalacturonic acid, trigalacturonic acid and tetragalacturonic acid after 10 min. After 2 h, tetragalacturonic acid disappears with concomitant accumulation of galacturonate | ? | |
| polygalacturonate + H2O | - |
Bacillus sp. (in: Bacteria) No. P-4-N | oligogalacturonates | liberation of digalacturonic acid, trigalacturonic acid and tetragalacturonic acid after 10 min. After 2 h, tetragalacturonic acid disappears with concomitant accumulation of galacturonate | ? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | 50°C: about 30% of maximal activity, 70°C: about 70% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
5 min, 80% of the activity remains | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | 10.5 | - |
Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 12 | pH 8.5: about 30% of maximal activity, pH 12.0: about 40% of maximal activity | Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
70°C, 10 min, 40% loss of activity | Bacillus sp. (in: Bacteria) |
| 6.5 | - |
70°C, 10 min, most stable at | Bacillus sp. (in: Bacteria) |
| 11 | - |
70°C, 10 min, 70% loss of activity | Bacillus sp. (in: Bacteria) |
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