3.2.1.15: endo-polygalacturonase
This is an abbreviated version!
For detailed information about endo-polygalacturonase, go to the full flat file.
Word Map on EC 3.2.1.15
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3.2.1.15
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aspergillus
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pepsinogen
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polygalacturonic
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niger
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galacturonic
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tomato
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pylori
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ripening
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citrus
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helicobacter
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gastritis
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fusarium
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polygalacturonase-inhibiting
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pgips
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methylesterase
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pectinases
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botrytis
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homogalacturonan
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cinerea
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alternaria
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peach
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macer
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sclerotiorum
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rhamnogalacturonans
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softening
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exopolygalacturonase
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sclerotinia
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ripe
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moniliforme
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pectolytic
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oligogalacturonates
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carotovora
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marxianus
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methylesterified
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food industry
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wall-degrading
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pylori-positive
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purpureum
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lindemuthianum
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methylesterification
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stereum
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cochliobolus
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pectinesterase
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polyuronides
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de-esterification
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pectin-degrading
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agriculture
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lycopersici
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nutrition
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medicine
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industry
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analysis
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degradation
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paper production
- 3.2.1.15
- aspergillus
- pepsinogen
- polygalacturonic
- niger
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galacturonic
- tomato
- pylori
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ripening
- citrus
- helicobacter
- gastritis
- fusarium
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polygalacturonase-inhibiting
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pgips
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methylesterase
- pectinases
- botrytis
- homogalacturonan
- cinerea
- alternaria
- peach
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macer
- sclerotiorum
- rhamnogalacturonans
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softening
- exopolygalacturonase
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sclerotinia
-
ripe
- moniliforme
-
pectolytic
- oligogalacturonates
- carotovora
- marxianus
-
methylesterified
- food industry
-
wall-degrading
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pylori-positive
- purpureum
- lindemuthianum
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methylesterification
- stereum
- cochliobolus
- pectinesterase
- polyuronides
-
de-esterification
-
pectin-degrading
- agriculture
-
lycopersici
- nutrition
- medicine
- industry
- analysis
- degradation
- paper production
Reaction
Synonyms
ADPG1, ADPG2, Aj-PGase, alkaline endo-PG, ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1, At1g48100, BcPeh28A, BPN3, CluPG1, D-galacturonase, endo PG, endo-D-galacturonanase, endo-D-galacturonase, endo-PG, endo-PG I, endo-pgaA, endo-polygalacturonase, endo-polygalacturonase A, endo-polygalacturonase C, endo-polygalacturonase I, endo-polygalacturonase II, endo-polygalacturonase-3, endo-TePG28b, endogalacturonase, endopectinase, endoPG, EndoPG I, endoPG II, endopolygalacturonase, endopolygalacturonase I, endopolygalacturonases, endopolygalacturonate lyase, EnPG28A, EPG, Epg1-2p, EPG4, Exo-PG, exo-polygalacturonase, FgPG, FpPG, liquifying polygalacturonase, LLP-A1.1 protein, LLP-PG, Magnaporthe oryzae density dependent germination regulator, male fertility 9, MDG1, MF9, More, MpPG2, non-acidic polygalacturonase, OGH, oligogalacturonate hydrolase, P1/P3, P2C, pectate hydrolase, pectic depolymerase, pectic hydrolase, pectin depolymerase, pectin hydrolase, pectin polygalacturonase, pectinase, pectinase SS, pectolase, pectozyme, Peh28A, PehA, PehB, PG, PG II, PG-2A, PG-3, PG1, PG2, PG28A, PG3, PG4, PG5, PG6, PG63, PG8fn, PGA, PGase, PGase SM, PGC, PGI, PGII, PGIP, PGL, Pgu1, phylendonase, poly [1, 4-alpha-D-galacturonide] glycano-hydrolase, poly-alpha-1,4-galacturonide glycanohydrolase, poly-[1,4-alpha-D-galacturonide] glycanohydrolase, polygalacturonase 1, polygalacturonase I, polygalacturonase II, polygalacturonase inhibiting protein 1, polygalacturonase p36, polygalacturonase p40, polygalacturonase-3, polygalacturonase-inhibitor-like, QRT2, QUARTET2, remanase, RPG1, ScPgu1, SpPgu1
ECTree
3.2.1.15 endo-polygalacturonaseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 3.2.1.15 - endo-polygalacturonase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
10 - 30
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the enzyme remains stable for 2 h at 10-30°C and shows about 50% and 25% activity after 2 h at 40°C and 60°C, respectively
100
2 - 75
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polygalacturonase is highly active at 20 and 37°C but is also detectable at 2, 50, and 75°C. No activity is detected following incubation in boiling water for 15 s
20 - 65
about 10% relative activity at 20°C, about 30% relative activity at 30°C, about 40% relative activity at 40°C, about 70% relative activity at 50°C, about 60% relative activity at 60°C, about 40% relative activity at 65°C
30
30 - 50
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the enzyme retains about 90%, 80%, 75% and 10% activity after 7 h at 30°C, 35°C, 40°C, and 50°C, respectively
30 - 56
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the enzyme retains about 90% activity after 30 min at 30-45°C. The activity drops to about 70%, 40% and less than 10% after 30 min at 45°C, 50°C and 55°C, respectively. The melting temperature is 56°C
30 - 60
the enzyme activity remains stable (more than 90% activity) after 30 min between 30 and 60°C
35 - 45
purified recombinant enzyme, pH 6.0, 1 h without substrate, completely stable
35 - 70
the enzyme retains more than 50% of its activity after incubation at 35-70°C for 60 min, but no activity remains after incubation at 70°C for 2 h
40
40 - 60
45
the enzyme retains more than 90% of the initial activity after incubation at 45°C for 1 h
50
50 - 60
50 - 75
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thermal treatment at 50°C does not have a significant effect on the activity of polygalacturonase in tomato juice, at temperatures between 50 and 75°C, thermal treatment results in partial enzyme inactivation, isoform PG2 is heat-labile and is totally inactivated after a 5 min treatment at 65°C. Thermosonication enhances the inactivation kinetics of isoform PG2 4fold at 60°C while the enhancement decreases to 2.3fold at 75°C
55
60
60 - 100
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the enzyme shows full polygalacturonase activity for up to 30 min at 60°C after which it experiences a sharp drop, the polygalacturonase retains full activity at 80°C for 5 min and has 50% activity at 70°C at 30 min. The enzyme is stable for 15 min at 70°C, while at 90°C for 15 min 35% activity remains. The least polygalacturonase enzyme activity is recorded at 100°C and 10 min (40%). After this, no activity is recorded
60 - 80
the enzyme retains about 70%, 40% and 10% activity after 1 h at 60°C, 70°C and 80°C, respectively
65
70
75
80
90
additional information
100
-
the purified enzyme is heat labile as boiling for 5 min reduces approximately 80% of activity
30
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stable up to 30°C, exhibits 49% of its activity at 20°C, stability decreases rapidly above 60°C
30
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about 40% inactivation of polygalacturonase I after 60 min, about 10% inactivation of polygalacturonase II after 60 min
30
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approximately stable up to 30°C for 60 min of incubation. Activity of enzyme is gradually decreased with increasing temperature and time
40
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about 55% inactivation of polygalacturonase I after 60 min, about 50% inactivation of polygalacturonase II after 60 min
40
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15 min, PG1 loses about 30% of its activity, PG2 loses about 10% of its activity
40 - 60
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the enzyme retains more than 60% of its initial activity after incubation at 40°C for 180 min. The enzyme rapidly loses activity at 50°C and 60°C
40 - 60
the enzyme retains 100%, 100%, 80% and 30% activity after 1 h at 40°C, 50°C, 55°C, and 60°C, respectively
40 - 60
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the enzyme remains stable for 2 h at 40°C and loses about 40% an 90% of its initial activity after 2 h at 50°C and 60°C, respectively. The enzyme is completely inactive after 3 h at 60 or 70°C
50
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complete inactivation of polygalacturonase I after 10 min, complete inactivation of polygalacturonase II after 40 min
50
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the enzyme presents a half-life of 2 h at 50°C. The enzyme is fully thermostable up to 45°C, whereas 25% of the initial activity is retained after 210 min of incubation at 50°C
50
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purified enzyme, pH 4.0-5.0, 120 min, stable, rapid inactivation above
50
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the purified enzyme is 100% stable at 50°C for 1 h, half-life of 10 min at 60°C. At 60°C, enzymatic activity decreases to only 20% of original activity after 1 h
50
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15 min, PG1 loses about 80% of its activity, PG2 loses about 10% of its activity
50 - 60
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purified enzyme, completely stable for 1 h at 50°C. At 55°C and 60°C, the activity decreases by 55% and 90%, respectively. After 50 min inactivation at 60°C
55
purified recombinant enzyme, pH 6.0, 1 h without substrate,loss of 70% activity
55
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after 50 min of incubation in citrus pectin, it maintains 80% of its activity, half-life at 90 min. Half-life in orange waste is only 10 min
60
the enzyme retains more than 90% activity after 30 min at 60°C
60
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purified recombinant proteins, pH 5.0, the wild-type full-length enzyme is completely stable up to, the catalytic domain of polygalacturonase loses 30% within 1 h
60
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the enzyme shows a high level of thermostability in the presence of substrate with a residual activity of 48 and 31% at 60°C after 2 and 3 h of incubation. Residual activity of 5% even after 6 h of incubation at 80°C
60
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above, purified enzyme, pH 5.0, 48.2% activity remaining after 10 min
60
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5 min, complete loss of activity of enzyme form PG2, about 5% loss of activity of enzyme form PG1
60
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15 min, PG1 loses about 90% of its activity, PG2 loses about 20% of its activity
70
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above, purified enzyme, pH 5.0, 9.3% activity remaining after 10 min
70
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15 min, PG1 loses about 95% of its activity, PG2 loses about 85% of its activity
80
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50% activity after 1.6 h at 50°C, 50% activity after 33 min at 55°C, 96% loss of activity after 30 min at 80°C
additional information
Acrocylindrium sp.
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polygalacturonic acid protects the enzyme from heat inactivation
additional information
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PG1 is stable at 50°C, at 70°C stability is drastically reduced. PG2 is a very sensitive enzyme, its half-life time at 50°C is in the range of min and at 70°C the enzyme is inactivated within 1 min
additional information
the enzyme is thermolabile, being completely inactivated if kept at 50°C for 2 h
additional information
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the enzyme is thermolabile, being completely inactivated if kept at 50°C for 2 h
additional information
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removal of large amounts of carbohydrate does not affect heat stability
additional information
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thermal and high-pressure inactivation kinetics of purified PG2 at pH 4.4
additional information
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inactivation at around 55°C corresponds to PG2 and at around 80°C corresponds to PG1. After 5 min at 90°C almost complete inactivation
