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Literature summary for 3.2.1.15 extracted from
- Expression, purification, and analyses of glycosylation and disulfide bonds of Stereum purpureum endopolygalacturonase I in Pichia pastoris (2009), Protein Expr. Purif., 65, 15-22.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | recombinant EndoPG Is are randomly glycosylated at four N-glycosylation sites | Chondrostereum purpureum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ligated into vector pPICZalpha A, transformed into competent cells of Escherichia coli JM109. Expressed in Pichia pastoris GS115 strain | Chondrostereum purpureum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36430 | 36750 | MALDI-TOF-MS analysis | Chondrostereum purpureum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chondrostereum purpureum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | recombinant EndoPG is with differing numbers of sugar chains (1-4), purified by ammonium sulfate precipitation and cation exchange chromatography, between 22.4-32.6fold | Chondrostereum purpureum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.7 | - |
culture supernatant of EndoPG I | Chondrostereum purpureum |
| 307 | - |
22.4fold purified recombinant EndoPG I with four sugar chains | Chondrostereum purpureum |
| 408 | - |
29.8fold purified recombinant EndoPG I with one sugar chain | Chondrostereum purpureum |
| 418 | - |
30.5fold purified recombinant EndoPG I with three sugar chains | Chondrostereum purpureum |
| 447 | - |
32.6fold purified recombinant EndoPG I with two sugar chains | Chondrostereum purpureum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid + H2O | - |
Chondrostereum purpureum | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EndoPG I | - |
Chondrostereum purpureum |
| endopolygalacturonase I | - |
Chondrostereum purpureum |
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