antisense RNA transgenic plants that display decreased expression of MF9 show pollen morphological defects that result in reduced pollen germination efficiency. Inhibition of MF9 also results in break-up of the previously formed tectum and baculae from the beginning of the binucleate stage as a result of premature degradation of tapetum
the ancestral form of polygalacturonase in Rhizopus is endolytic, and exolytic function evolved later, phylogenetic tree of endo-and exolytic polygalacturonases in Rhizopus oryzae, overview
the ancestral form of polygalacturonase in Rhizopus is endolytic, and exolytic function evolved later, phylogenetic tree of endo-and exolytic polygalacturonases in Rhizopus oryzae, overview
MF9 may act as a co-ordinator in the late stages of tapetum degeneration, and subsequently in the regulation of wall material secretion and, in turn, exine formation. BcMF9 may also play a role in intine formation, possibly via regulation of the dynamic metabolism of pectin
contribution of the endo-PG protein to softening during peach ripening. Changes in tissue morphology, endo-PG localization, and endo-PG isoforms are examined during ripening in melting flesh and non-melting flesh cultivars, as well as in a slow-ripening mutant, overview
in abscission, the enzyme is responsible for the degradation of the middle lamella and for the loosening of the primary cell wall. Deposition of certain substances, such as xyloglucan and galactan for floral abscission and lignin for fruit, occurs specifically at the abscission zone
infiltration of the enzyme into Arabidopsis thaliana induces a necrotic response mediated via recognition of the enzyme by resposiveness to Bortrytis polygalacturonases 1, RBPG1, a leucine-rich repeat receptor-like protein, AtRLP42, which acts as a microbe-associated molecular pattern receptor. Most sever effects occur in Arabidopsis thaliana accessions Col-0, Kas-1, and Kas-2, but not in accessions Br-0 and Est-0, but responsiveness is a dominant trait, detailed genetic analysis of accession responsiveness to the different isozymes, overview. RBPG1 homologues physically interact with isozyme BcPG3 and Arabidopsis leucine-rich repeat receptor-like protein, SOBIR1
the enzyme reduces the viscosity of papaya juice by 17.6% and increases its transmittance by 59.1%. The enzyme works synergistically with pectin methylesterase
isoform PG2 performs a regulatory role on the inducible isoform pg1 gene and in triggering the plant immune response during the interaction with tomato roots
the enzyme reduces the viscosity of papaya juice by 17.6% and increases its transmittance by 59.1%. The enzyme works synergistically with pectin methylesterase
isoform PG2 performs a regulatory role on the inducible isoform pg1 gene and in triggering the plant immune response during the interaction with tomato roots
comparison of endo-polygalacturonase from Saccharomyces cerevisiae with the enzyme from Saccharomyces paradoxus after overexpression in Saccharomyces cerevisiae under wine production conditions, overview. The higher activity of Saccharomyces paradoxus strains under laboratory conditions might be due to a different regulation mechanism rather than to a different sequence of gene PGU1. ScPgu1 displayed higher Km and Vmax than SpPgu1 thereby showing a slightly lower affinity for the substrate but a twofold stronger activity under optimal conditions
comparison of endo-polygalacturonase from Saccharomyces cerevisiae with the enzyme from Saccharomyces paradoxus after overexpression in Saccharomyces cerevisiae under wine production conditions, overview. The higher activity of Saccharomyces paradoxus strains under laboratory conditions might be due to a different regulation mechanism rather than to a different sequence of gene PGU1. ScPgu1 displayed higher Km and Vmax than SpPgu1 thereby showing a slightly lower affinity for the substrate but a twofold stronger activity under optimal conditions
comparison of endo-polygalacturonase from Saccharomyces cerevisiae with the enzyme from Saccharomyces paradoxus after overexpression in Saccharomyces cerevisiae under wine production conditions, overview. The higher activity of Saccharomyces paradoxus strains under laboratory conditions might be due to a different regulation mechanism rather than to a different sequence of gene PGU1. ScPgu1 displayed higher Km and Vmax than SpPgu1 thereby showing a slightly lower affinity for the substrate but a twofold stronger activity under optimal conditions
comparison of endo-polygalacturonase from Saccharomyces cerevisiae with the enzyme from Saccharomyces paradoxus after overexpression in Saccharomyces cerevisiae under wine production conditions, overview. The higher activity of Saccharomyces paradoxus strains under laboratory conditions might be due to a different regulation mechanism rather than to a different sequence of gene PGU1. ScPgu1 displayed higher Km and Vmax than SpPgu1 thereby showing a slightly lower affinity for the substrate but a twofold stronger activity under optimal conditions
the wild-type strain ToUdk2 contains endo-PG PehA, EC 3.2.1.15, and exo-PGs PehB and PehC, EC 3.2.1.67, while the PC mutant has mainly endo-PG activity. Endo- and exo-PG synthesis is governed by a sensitive regulatory network, which, in interaction with inhibitory polygalacturonase-inhibiting proteins and cell wall degradation products, leads to generation or avoidance of elicitor-active oligomers, and, thus, may contribute to the development of the compatible or incompatible interaction
active site cleft residues are identified: D210 for MpPG2 as general acid catalyst, residues D190/D211, as general base catalyst, and residue H232 which is involved in the regeneration of the acid-base equilibrium of the catalytic aspartate
active site cleft residues are identified: D210 for MpPG2 as general acid catalyst, residues D190/D211, as general base catalyst, and residue H232 which is involved in the regeneration of the acid-base equilibrium of the catalytic aspartate
the multimodular enzyme consists of an N-terminal catalytic domain of pectin methylesterase, a Thr/Ser-rich linker region, and a C-terminal catalytic domain of polygalacturonase
the multimodular enzyme consists of an N-terminal catalytic domain of pectin methylesterase, a Thr/Ser-rich linker region, and a C-terminal catalytic domain of polygalacturonase
comparison of endo-polygalacturonase from Saccharomyces cerevisiae with the enzyme from Saccharomyces paradoxus after overexpression in Saccharomyces cerevisiae under wine production conditions, overview. The higher activity of Saccharomyces paradoxus strains under laboratory conditions might be due to a different regulation mechanism rather than to a different sequence of gene PGU1. ScPgu1 displayed higher Km and Vmax than SpPgu1 thereby showing a slightly lower affinity for the substrate but a twofold stronger activity under optimal conditions
the wild-type strain ToUdk2 contains endo-PG PehA, EC 3.2.1.15, and exo-PGs PehB and PehC, EC 3.2.1.67, while the PC mutant has mainly endo-PG activity. Endo- and exo-PG synthesis is governed by a sensitive regulatory network, which, in interaction with inhibitory polygalacturonase-inhibiting proteins and cell wall degradation products, leads to generation or avoidance of elicitor-active oligomers, and, thus, may contribute to the development of the compatible or incompatible interaction
comparison of endo-polygalacturonase from Saccharomyces cerevisiae with the enzyme from Saccharomyces paradoxus after overexpression in Saccharomyces cerevisiae under wine production conditions, overview. The higher activity of Saccharomyces paradoxus strains under laboratory conditions might be due to a different regulation mechanism rather than to a different sequence of gene PGU1. ScPgu1 displayed higher Km and Vmax than SpPgu1 thereby showing a slightly lower affinity for the substrate but a twofold stronger activity under optimal conditions