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3.2.1.15: endo-polygalacturonase

This is an abbreviated version!
For detailed information about endo-polygalacturonase, go to the full flat file.

Word Map on EC 3.2.1.15

Reaction

(1,4-alpha-D-galacturonosyl)n+m
+
H2O
=
(1,4-alpha-D-galacturonosyl)n
 +
(1,4-alpha-D-galacturonosyl)m

Synonyms

ADPG1, ADPG2, Aj-PGase, alkaline endo-PG, ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1, At1g48100, BcPeh28A, BPN3, CluPG1, D-galacturonase, endo PG, endo-D-galacturonanase, endo-D-galacturonase, endo-PG, endo-PG I, endo-pgaA, endo-polygalacturonase, endo-polygalacturonase A, endo-polygalacturonase C, endo-polygalacturonase I, endo-polygalacturonase II, endo-polygalacturonase-3, endo-TePG28b, endogalacturonase, endopectinase, endoPG, EndoPG I, endoPG II, endopolygalacturonase, endopolygalacturonase I, endopolygalacturonases, endopolygalacturonate lyase, EnPG28A, EPG, Epg1-2p, EPG4, Exo-PG, exo-polygalacturonase, FgPG, FpPG, liquifying polygalacturonase, LLP-A1.1 protein, LLP-PG, Magnaporthe oryzae density dependent germination regulator, male fertility 9, MDG1, MF9, More, MpPG2, non-acidic polygalacturonase, OGH, oligogalacturonate hydrolase, P1/P3, P2C, pectate hydrolase, pectic depolymerase, pectic hydrolase, pectin depolymerase, pectin hydrolase, pectin polygalacturonase, pectinase, pectinase SS, pectolase, pectozyme, Peh28A, PehA, PehB, PG, PG II, PG-2A, PG-3, PG1, PG2, PG28A, PG3, PG4, PG5, PG6, PG63, PG8fn, PGA, PGase, PGase SM, PGC, PGI, PGII, PGIP, PGL, Pgu1, phylendonase, poly [1, 4-alpha-D-galacturonide] glycano-hydrolase, poly-alpha-1,4-galacturonide glycanohydrolase, poly-[1,4-alpha-D-galacturonide] glycanohydrolase, polygalacturonase 1, polygalacturonase I, polygalacturonase II, polygalacturonase inhibiting protein 1, polygalacturonase p36, polygalacturonase p40, polygalacturonase-3, polygalacturonase-inhibitor-like, QRT2, QUARTET2, remanase, RPG1, ScPgu1, SpPgu1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.15 endo-polygalacturonase

pH Stability

pH Stability on EC 3.2.1.15 - endo-polygalacturonase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1
-
notable loss of activity irrespective of the period of exposure
135810
1 - 6.5
-
after 2 h of incubation, the purified enzyme retains more than 75% of its initial activity between the pH values of 2.0 and 6.5, and shows up to 55% residual activity within a very low pH interval (1.0-2.0). After 24 h in the pH range of 1.0-3.0 the enzyme retains 35% of its initial activity at pH 1.0 and more than 75% from pH 1.5 onwards
751362
1 - 8
-
stable for several hours, marked loss of activity after 6 days
135810
11
-
70°C, 10 min, 70% loss of activity
135835
2 - 6
purified recombinant enzyme, 1 h, 37°C, over 60% activity remaining
717624
2 - 7
the enzyme remains stable after 1 h at pH 2.0-7.0
751307
2 - 8
the enzyme retains about 80% activity after 1 h incubation at pH 2.0-8.0
750585
2 - 9
-
more than 80% of enzyme activity is retained after incubation in pH 2.0 to 6.0 for 24 h. A marked decrease in enzyme activity is observed at pH 7.0 to 9.0
750192
2.2 - 7
the enzyme retains more than 90% activity after 1 h at pH 2.2-7.0 and 37°C
749703
2.5 - 6
-
20°C, 20 h, activity is completely stable
655368
2.5 - 7.5
Acrocylindrium sp.
-
30°C, 24 h, stable
135834
2.8
-
2 h, about 60% loss of activity
135824
3 - 10
the enzyme retains more than 80% enzyme activity at pH 3.0-10.0 when it is incubated at 25°C for 1 h, while it retains more than 80% enzyme activity at pH 4.0-6.0 when it is incubated at 25°C for 24 h
751140
3 - 4.5
-
purified enzyme, 25°C, 24 h, stable at
728968
3 - 5
-
stable
654226
3 - 6
3 - 6.5
-
purified enzyme, 49°C, 10 h, stable at
729513
3 - 7
3.5
-
2 h, about 25% loss of activity
135824
3.5 - 6
3.5 - 8
purified recombinant enzyme, 37°C, 1 h without substrate, over 75% activity remaining
729770
4 - 5
-
4°C or 30°C, stable
135833
4 - 5.5
-
purified enzyme, 1 h, stable at
730876
4 - 6
4 - 7
Saccharomyces fragilis
-
37°C, 30 min, stable
135812
4 - 8
4 - 9
4.3
-
polygalacturonase I is most stable at
135832
4.5
-
70°C, 10 min, 40% loss of activity
135835
4.5 - 8
-
more than 90% of PG activity remains after 24 h in citrus pectin and orange waste, incubation in orange waste gives better results than incubation in citrus pectin at extreme pHs
677638
5 - 6.5
-
in the absence of substrate, the purified enzyme is stable in a narrow pH range, with 100% stability at 5.0, although 55% of this activity is retained at pH 6.5. It maintains 33% of initial activity at pH 9.0
677609
5 - 7
-
30°C, 10 h, stable
135853
5.6
-
polygalacturonase II is most stable at
135832
6
-
2 h, about 10% loss of activity
135824
6.5
-
70°C, 10 min, most stable at
135835
7 - 11
-
the enzyme remains stable for 2 h at pH 7.0-11.0
749438
8.1 - 11.5
-
the enzyme reserves over 65% of the initial activity at a pH range of 8.1-11.5 after incubating the sample at 4°C for 24 h
750075