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3.2.1.15: endo-polygalacturonase

This is an abbreviated version!
For detailed information about endo-polygalacturonase, go to the full flat file.

Word Map on EC 3.2.1.15

Reaction

(1,4-alpha-D-galacturonosyl)n+m
+
H2O
=
(1,4-alpha-D-galacturonosyl)n
 +
(1,4-alpha-D-galacturonosyl)m

Synonyms

ADPG1, ADPG2, Aj-PGase, alkaline endo-PG, ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1, At1g48100, BcPeh28A, BPN3, CluPG1, D-galacturonase, endo PG, endo-D-galacturonanase, endo-D-galacturonase, endo-PG, endo-PG I, endo-pgaA, endo-polygalacturonase, endo-polygalacturonase A, endo-polygalacturonase C, endo-polygalacturonase I, endo-polygalacturonase II, endo-polygalacturonase-3, endo-TePG28b, endogalacturonase, endopectinase, endoPG, EndoPG I, endoPG II, endopolygalacturonase, endopolygalacturonase I, endopolygalacturonases, endopolygalacturonate lyase, EnPG28A, EPG, Epg1-2p, EPG4, Exo-PG, exo-polygalacturonase, FgPG, FpPG, liquifying polygalacturonase, LLP-A1.1 protein, LLP-PG, Magnaporthe oryzae density dependent germination regulator, male fertility 9, MDG1, MF9, More, MpPG2, non-acidic polygalacturonase, OGH, oligogalacturonate hydrolase, P1/P3, P2C, pectate hydrolase, pectic depolymerase, pectic hydrolase, pectin depolymerase, pectin hydrolase, pectin polygalacturonase, pectinase, pectinase SS, pectolase, pectozyme, Peh28A, PehA, PehB, PG, PG II, PG-2A, PG-3, PG1, PG2, PG28A, PG3, PG4, PG5, PG6, PG63, PG8fn, PGA, PGase, PGase SM, PGC, PGI, PGII, PGIP, PGL, Pgu1, phylendonase, poly [1, 4-alpha-D-galacturonide] glycano-hydrolase, poly-alpha-1,4-galacturonide glycanohydrolase, poly-[1,4-alpha-D-galacturonide] glycanohydrolase, polygalacturonase 1, polygalacturonase I, polygalacturonase II, polygalacturonase inhibiting protein 1, polygalacturonase p36, polygalacturonase p40, polygalacturonase-3, polygalacturonase-inhibitor-like, QRT2, QUARTET2, remanase, RPG1, ScPgu1, SpPgu1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.15 endo-polygalacturonase

Cloned

Cloned on EC 3.2.1.15 - endo-polygalacturonase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
1620 bp DNA fragment encompassing the upstream 218 bp plus the entire coding region of the Xc17 pehA into the PstI-XbaI sites of the broad-host-range vector pRK415. 1376 bp NdeI-XhoI fragment containing the Xc17 pehA gene cloned in the Escherichia coli vector pOK12, giving pOKpeh. A Gmr cartridge from pUCGM inserted into the unique HincII site within the pOKpeh insert. Resultant plasmid, pOKpehG, electroporated into strain Xc17 allowing for double crossover. PpehA-lacZ transcriptional fusion constructs generated by cloning PCR fragments into the broad-host-range promoter-probing vector pFY13-9, which uses lacZ as the reporter
-
BcMF2 cloned in the antisense orientation into the binary vector pBI121 and transferred into the Chinese cabbage
-
cloned into the Escherichia coli/yeast shuttle vector Yepsec1 for secretion in Saccharomyces cerevisiae
-
cloning in Escherichia coli strain TOP10F, expression in Pichia pastoris strain X-33 and secretion to the culture medium
-
DNA and amino acid sequence determination and analysis, overexpression in Saccharomyces cerevisiae strain VIN13, which is itself completely devoid of endo-polygalacturonase activity, and secretion to the medium
expressed in Arabidopsis thaliana
-
expressed in Escherichia coli Rosetta-gami 2 cells
expressed in Nicotiana tabacum and Arabidopsis thaliana
-
expressed in Pichia pastoris
expressed in Pichia pastoris GS115 cells
expressed in Pichia pastoris strain GS115
-
expressed in Pichia pastoris strain KM71
-
expressed in Pichia pastoris. PVX as a vector to systemically express endoPG in Nicotiana benthamiana, the coding sequence downstream of the signal peptide of endoPG gene subcloned into pgR106:SP, in-frame with the signal peptide of tobacco PR1a, for secretion of the recombinant protein into the apoplast
expressed in Saccharomyces cerevisiae strain EBY100
expressed in Saccharomyces cerevisiae strains UCLMS-1, -3 and -4, while strains Collection Cepage Cabernet and L2226 do not show any transcription of the gene after integration
-
expression in Escherichia coli
-
expression in Escherichia coli strain Origami (DE3) provides a soluble and active enzyme with proper disulfide bond formation, the enzyme expressed in BL21 is localized in inclusion bodies
-
expression of 10xMyc-tagged isozyme BcPG3 in Nicotiana benthamiana, Arabidopsis thaliana protein RBPG1 and its homologues form with isozyme BcPG3 when co-expressed in Nicotiana benthamiana
-
gene pg I, DNA and amino acid sequence determination and analysis, overexpression in Pichia pastoris in fed-batch fermentation
gene pg I, DNA and amino acid sequence determination and analysis, recombinant expression in Pichia pastoris strain GS115
generation of transgenic Arabidopsis thaliana Ws and Nicotiana tabacum Petit Havana-SR1 plants expressing PG with the point mutation N178D. Arabidopsis line PG201 expressing PGII with the point mutation D201N
-
GhPS1:eGFP fusion gene cloned into pBI121 vector at BamHI/SacI sites, replacing the GUS gene. Hypocotyl explants of cotton (cv Coker 312) transformed with chimeric GhPS1:eGFP fusion gene
heterologously expressed
-
highly conserved region in the C-terminal region of the enzyme probably comprising the elements of substrate binding and the catalytic centre
-
His-ADPG1, His-ADPG2, and His-At1g48100 proteins expressed in Escherichia coli. ADPG1, ADPG2, and QRT2, promoter:GUS transcriptional constructs transformed into wild-type plants
-
homologously overexpressed
-
into pBluescript KS
into plasmid pET22b and transformed into Escherichia coli strain DH5alpha, expressed in Escherichia coli BL21(DE3) cells
into the expression vector pBAD24 and transformed into Escherichia coli strain TOP10
into the pGEM-T-easy vector
-
into the pGEM1-T Easy vector, using DH5alpha Escherichia coli cells as the host
isozyme MpPG2, DNA and amino acid sequence determination and analysis
ligated into vector pPICZalpha A, transformed into competent cells of Escherichia coli JM109. Expressed in Pichia pastoris GS115 strain
-
LLP-PG cDNA cloned into pGEM-T Easy vector
multigene family encoding polygalacturonases consists of seven genes
-
overexpression in Escherichia coli
overexpression in Saccharomyces cerevisiae strain VIN13, which is itself completely devoid of endo-polygalacturonase activity, and secretion to the medium
PG1 and mutated form of PG1 transiently expressed in leaves of Nicotiana benthamiana. Coexpression of pgg2 and the pgip1 gene from Vitis vinifera in Nicotiana benthamiana
PG2 and mutated form of PG2 transiently expressed in leaves of Nicotiana benthamiana. Coexpression of pgg2 and the pgip1 gene from Vitis vinifera in Nicotiana benthamiana. PG2-D192A mutant produced in Pichia pastoris
PG3 transiently expressed in leaves of Nicotiana benthamiana
PG4 transiently expressed in leaves of Nicotiana benthamiana
PG5 transiently expressed in leaves of Nicotiana benthamiana
PG6 transiently expressed in leaves of Nicotiana benthamiana
phylogenetic tree of endo-and exolytic polygalacturonases in Rhizopus oryzae, recombinant expression in Pichia pastoris, subcloning in Escherichia coli strain TOP10
-
promoter regions of pghAxc and pghBxc cloned and fused with beta-glucuronidase genes
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recombinant enzyme expression
-
recombinant expression of the wild-type full-length enzyme and its catalytic domain of polygalacturonase in Pichia pastoris strain GS115
-
the pro-form of polygalacturonan I is expressed in Escherichia coli Origami (DE3) cells
-