Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | pehA transcription is greatly induced by polygalacturonic acid and positively regulated by Clp (cAMP receptor protein-like protein) and RpfF (an enoyl-CoA hydratase homologue which is required for the synthesis of cis-11-methyl-2-dodecenoic acid, a low-molecular-mass diffusible signal factor) | Xanthomonas campestris pv. campestris |
Application | Comment | Organism |
---|---|---|
additional information | PehA is the major but not the sole polygalacturonase, it plays a minor role in Xanthomonas campestris virulence | Xanthomonas campestris pv. campestris |
Cloned (Comment) | Organism |
---|---|
1620 bp DNA fragment encompassing the upstream 218 bp plus the entire coding region of the Xc17 pehA into the PstI-XbaI sites of the broad-host-range vector pRK415. 1376 bp NdeI-XhoI fragment containing the Xc17 pehA gene cloned in the Escherichia coli vector pOK12, giving pOKpeh. A Gmr cartridge from pUCGM inserted into the unique HincII site within the pOKpeh insert. Resultant plasmid, pOKpehG, electroporated into strain Xc17 allowing for double crossover. PpehA-lacZ transcriptional fusion constructs generated by cloning PCR fragments into the broad-host-range promoter-probing vector pFY13-9, which uses lacZ as the reporter | Xanthomonas campestris pv. campestris |
Protein Variants | Comment | Organism |
---|---|---|
additional information | in mutant MH172 with cloned pehA, MH172(pRKpeh), wild-type-level polygalacturonase activity is restored | Xanthomonas campestris pv. campestris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | expression of pehA is subject to catabolite repression, which is independent of Clp and RpfF, and it is repressed under conditions of oxygen limitation or nitrogen starvation | Xanthomonas campestris pv. campestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris pv. campestris | - |
- |
- |
Xanthomonas campestris pv. campestris Xc17 | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
PehA | - |
Xanthomonas campestris pv. campestris |