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Literature summary for 3.2.1.15 extracted from

  • Ogawa, M.; Kay, P.; Wilson, S.; Swain, S.M.
    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and QUARTET2 are polygalacturonases required for cell separation during reproductive development in Arabidopsis (2009), Plant Cell, 21, 216-233.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information IND and HEC3 are required for normal expression of ADPG1 in the silique dehiscence zone and seed abscission zone, respectively. Transcriptional regulation of ADPG1, ADPG2 and QRT2 by jasmonic acid, and of ADPG2 by ethylene. Jasmonic acid and ethylene act together with abscisic acid to regulate floral organ abscission, in part by promoting QRT2 expression Arabidopsis thaliana

Application

Application Comment Organism
additional information ADPG1 and ADPG2 are essential for silique dehiscence. ADPG2 and QRT2 contribute to floral organ abscission, while all three genes contribute to anther dehiscence Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
His-ADPG1, His-ADPG2, and His-At1g48100 proteins expressed in Escherichia coli. ADPG1, ADPG2, and QRT2, promoter:GUS transcriptional constructs transformed into wild-type plants Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information under normal growing conditions, single adpg2 mutants appear similar to wild-type plants in terms of pod shatter and also produce monad pollen. Reduced pod shatter in adpg2-1 and adpg2-2 plants (and in double mutants with qrt2) when watering is ceased before overall plant senescence is complete. Siliques of the double-mutants adpg1-1 adpg2-1 and adpg1-2 adpg2-2 exhibit a more severe phenotype than do those of the adpg1 single mutants and fail to dehisce even if compressed. In terms of pod shatter and seed abscission, adpg2 qrt2 double mutants are similar to adpg2 single mutants, double mutants lacking both ADPG1 and QRT2 appear identical to adpg1 single mutants, and the adpg1-1 adpg2-1 qrt2-2 and adpg1-2 adpg2-2 qrt2-3 triple mutants resemble the adpg1 adpg2 double mutants Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower bud high levels of ADPG1, ADPG2 present and low levels of QRT2 Arabidopsis thaliana
-
additional information QRT2:GUS is expressed in the vascular bundles in the replum and at the central region of the valve. ADPG1-GUS is expressed in stomium cells Arabidopsis thaliana
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root ADPG2 and high levels of QRT2 Arabidopsis thaliana
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rosette leaf low levels of QRT2 Arabidopsis thaliana
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seed
-
Arabidopsis thaliana
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silique ADPG1 is present in siliques just before pod shatter. ADPG2 is expressed in mature siliques. Low levels of QRT2 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.025
-
ADPG1 Arabidopsis thaliana
0.679
-
ADPG2 Arabidopsis thaliana
2.713
-
At1g48100 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
Arabidopsis thaliana oligogalacturonides
-
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Synonyms

Synonyms Comment Organism
ADPG1
-
Arabidopsis thaliana
ADPG2
-
Arabidopsis thaliana
ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1
-
Arabidopsis thaliana
At1g48100
-
Arabidopsis thaliana
endo-PG
-
Arabidopsis thaliana
QRT2
-
Arabidopsis thaliana
QUARTET2
-
Arabidopsis thaliana