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Literature summary for 3.2.1.15 extracted from
- Characterization of an extracellular endopolygalacturonase from the saprobe Mucor ramosissimus Samutsevitsch and its action as trigger of defensive response in tropical plants (2006), Mycopathologia, 162, 337-346.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | besides their role in recycling organic matter, saprobiotic enzymes like endopolygalacturonase may also play an important role in the induction of defensive mechanisms in wild plants by enhancing their non-specific resistance against pathogens | Mucor ramosissimus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
x * 24000, SDS-PAGE | Mucor ramosissimus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mucor ramosissimus | - |
- |
- |
| Mucor ramosissimus URM 3087 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially purified by ion exchange chromatography, gel filtration, and preparative isoelectrofocusing, up to 207fold | Mucor ramosissimus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Mucor ramosissimus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 542 | - |
culture filtrate | Mucor ramosissimus |
| 112600 | - |
207fold purified enzyme | Mucor ramosissimus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, for 180 days | Mucor ramosissimus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | pectin from citrus or Palicourea marcgravii, more active upon pectic substrates with a low degree of methyl esterification. The enzyme itself and the products of its action on the pectic fraction of Palicourea marcgravii elicite the production of defensive compounds in the leaves of the plant | Mucor ramosissimus | oligogalacturonides | - |
? |  |
| pectin + H2O | pectin from citrus or Palicourea marcgravii, more active upon pectic substrates with a low degree of methyl esterification. The enzyme itself and the products of its action on the pectic fraction of Palicourea marcgravii elicite the production of defensive compounds in the leaves of the plant | Mucor ramosissimus URM 3087 | oligogalacturonides | - |
? | |
| polygalacturonic acid + H2O | - |
Mucor ramosissimus | ? | - |
? | |
| polygalacturonic acid + H2O | - |
Mucor ramosissimus URM 3087 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24000, SDS-PAGE | Mucor ramosissimus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endopolygalacturonase | - |
Mucor ramosissimus |
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