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Literature summary for 3.2.1.15 extracted from

  • Miyairi, K.; Okuno, T.; Sawai, K.
    Purification and properties of endopolygalacturonase I from Stereum purpureum, a factor inducing silver-leaf symptoms on apple trees (1985), Agric. Biol. Chem., 49, 111-118.
No PubMed abstract available
Search for more literature for 3.2.1.15

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Chondrostereum purpureum
Cd2+
-
 Chondrostereum purpureum
Hg2+ 0.5 mM, complete inhibition Chondrostereum purpureum
Sn2+
-
 Chondrostereum purpureum
Sr2+
-
 Chondrostereum purpureum

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ 50 mM, stimulates Chondrostereum purpureum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Chondrostereum purpureum
41000
-
 1 * 41000, SDS-PAGE Chondrostereum purpureum
44000
-
 equilibrium sedimentation Chondrostereum purpureum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Chondrostereum purpureum the enzyme is a factor inducing silver-leaf symptoms on apple trees ?
-
 ?

Organism

Organism UniProt Comment Textmining
Chondrostereum purpureum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chondrostereum purpureum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
560
-
-
 Chondrostereum purpureum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Chondrostereum purpureum digalacturonic acid + galacturonate
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Chondrostereum purpureum alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + D-galacturonate
-
 ?
additional information the enzyme is a factor inducing silver-leaf symptoms on apple trees Chondrostereum purpureum ?
-
 ?
pectic acid + H2O
-
 Chondrostereum purpureum galacturonic acid oligomers
-
 ?
polygalacturonate + H2O
-
 Chondrostereum purpureum oligogalacturonates
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Chondrostereum purpureum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Chondrostereum purpureum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90 40°C: about 40% of maximal activity, 90°C: about 35% of maximal activity Chondrostereum purpureum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 30 min, stable up to Chondrostereum purpureum
75
-
 rapid inactivation Chondrostereum purpureum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 hydrolysis of trigalacturonic acid Chondrostereum purpureum
4
-
 hydrolysis of tetragalacturonic acid Chondrostereum purpureum
4.5
-
 hydrolysis of pectic acid Chondrostereum purpureum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 30 min, stable up to 70°C Chondrostereum purpureum