Literature summary for 3.2.1.15 extracted from

Jurick, W.M.; Vico, I.; McEvoy, J.L.; Whitaker, B.D.; Janisiewicz, W.; Conway, W.S.
Isolation, purification, and characterization of a polygalacturonase produced in Penicillium solitum-decayed Golden Delicious apple fruit (2009), Phytopathology, 99, 636-641.

Search for more literature for 3.2.1.15

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	85.1% residual activity at 1 mM	Malus domestica
Mn2+	87.3% residual activity at 1 mM	Malus domestica

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	130% relative activity at 1 mM	Malus domestica
Mg2+	111% relative activity at 1 mM	Malus domestica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	SDS-PAGE	Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
polygalacturonate + H2O	Malus domestica	-	oligogalacturonates	-	?

Organism

Organism	UniProt	Comment	Textmining
Malus domestica	-	cultivar Golden Delicious	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Sephacryl S-200 gel filtration, and CM-Sephadex gel filtration	Malus domestica

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	-	Malus domestica	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0319	-	crude extract, in 100 mM sodium acetate, pH 5.5, containing 0.4% polygalacturonic acid, at 20°C	Malus domestica
0.102	-	after purification, in 100 mM sodium acetate, pH 5.5, containing 0.4% polygalacturonic acid, at 20°C	Malus domestica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
polygalacturonate + H2O	-	Malus domestica	oligogalacturonates	-	?
sodium polypectate + H2O	-	Malus domestica	?	-	?

Synonyms

Synonyms	Comment	Organism
endopolygalacturonase	-	Malus domestica

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
2	75	polygalacturonase is highly active at 20 and 37°C but is also detectable at 2, 50, and 75°C. No activity is detected following incubation in boiling water for 15 s	Malus domestica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	5	-	Malus domestica

pH Range

pH Minimum	pH Maximum	Comment	Organism
3	7	-	Malus domestica

General Information

General Information	Comment	Organism
physiological function	polygalacturonase plays a role in Penicillium solitum-mediated decay of apple fruit	Malus domestica

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)