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3.1.26.4: ribonuclease H

This is an abbreviated version!
For detailed information about ribonuclease H, go to the full flat file.

Word Map on EC 3.1.26.4

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Endonucleolytic cleavage to a 5'-phosphomonoester =

Synonyms

Ape-RNase HII, aRNase HII, Bh ribonuclease H, BhRNase H, CtepRNH, EcRNH, endoribonuclease H, endoribonuclease H (calf thymus), ERNH, huRNaseH1, hybrid nuclease, hybrid ribonuclease, hybridase, hybridase (ribonuclease H), KOD1, LC9-RNase H1, lmo1273, LRNase HII, More, nuclease, hybrid ribo-, nuclease, ribo-, H, P32, protein ST0753, reverse transcriptase, ribonuclease H, ribonuclease H I, ribonuclease H(42), ribonuclease H(70), ribonuclease H1, ribonuclease H2, ribonuclease H3, ribonuclease HI, ribonuclease HII, ribonuclease HIII, ribonulease H1, RNA*DNA hybrid ribonucleotidohydrolase, RNA*DNA hybrid ribonucleotiohydrolase, RNase H, RNase H type 2, RNase H(35), RNase H1, RNase H2, RNase H3, RNase HI, RNase HII, RNase HIII, RNaseH, Rnaseh1, RNASEH2A, RNH, RNH1, RNH2, rnhA, RnhB, rnhC, rnhD, Rv2228c, ST0519, STK_07530, Sto-RNase HI, T4 RNase H, Tk-RNase HII, Tk-Tk-RNase HII, TK0805, TRNH, TthRNH, Ty1 reverse transcriptase/RNase H, type 1 ribonuclease H, type 1 RNase H, type 2 ribonuclease H, type 2 RNase H, type II ribonuclease H

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.4 ribonuclease H

Cloned

Cloned on EC 3.1.26.4 - ribonuclease H

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
complementation assay using the enzyme-deficient Escherichia coli strain MIC2067, which shows an RNase H-dependent temperature-sensitive growth phenotype, by wild-type and mutant enzymes, no complementation by the C domian mutant, overview
EcRNH, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of soluble GST-tagged enzyme in Escherichia coli strain BL21(DE3) plysS, subcloning in Escherichia coli strain DH5alpha
expressed in Escherichia coli BL-21cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)[pLysS] cells
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expressed in Escherichia coli BL21-CodonPlus(DE3) cells
expressed in Escherichia coli MIC2067(DE3) cells
expression in Escherichia coli
expression in Escherichia coli and in HeLa cell
expression of enzyme type 1 and 2 as GFP-fusion proteins in all 6 cell lines, transfection of cell lines with antisense oligodeoxynucleotides directed against RNA polymerase II, replication protein A, and Ha-ras, determination of response in expression levels of the enzyme type 1 and 2, overview
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expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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expression of His-tagged RNase H1 in Escherichia coli strain BL21(DE3)
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expression of human RNase H1 in Escherichia coli
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expression of mutant enzymes in Escherichia coli
Halalkalibacterium halodurans
expression of recombinant chimeric and point mutant enzymes in Escherichia coli strain BL21(DE3), I53 and L56 point mutants are expressed insolubly in inclusion bodies
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expression of recombinant chimeric mutant enzyme and of His-tagged mutant L56S in Escherichia coli strain BL21(DE3)
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expression of RNase HII and RNase HIII in the enzyme-deficient Escherichia coli strain DY329, complementation assay, Chlamydophila pneumoniae RNase HII can complement both Escherichia coli RNase HII and RNase HI, but Chlamydophila pneumoniae RNase HIII can only complement the latter
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expression of wild-type and mutant enzymes in Escherichia coli BL21(DE3)
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expression of wild-type and mutants enzymes in Escherichia coli BL21(DE3)
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expression of wild-type and N-terminally truncated mutant enzymes as GST-fusion proteins in Escherichia coli temperature sensitive mutant strain MIC3001
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expression of wild-type and truncated D107N mutant enzymes in Escherichia coli
gene PAB0352
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gene rnaseh1, translation initiates at each of the two in-frame AUGs of the Rnaseh1 mRNA, with the longer form being imported into mitochondria, regulation mechanisms, overview. Recombinant enzyme expression in Flp-In T-Rex-293 cells, and in vitro translation.
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gene rnhA, overexpression in Escherichia coli MIC3001 of wild-type and mutant enzymes, complementation of enzyme-dependent temperature-sensitive growth phenotype of the Escherichia coli host strain by expression of the wild-type enzyme, but only marginally by the mutant D134H
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gene rnhA, recombinant expression of wild-type and mutant His10-tagged enzymes in Escherichia coli
gene rnhA, recombinant overexpression of RNase H1 in Escherichia coli strain MIC3009
gene rnhB, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain MIC2067(DE3), a rnhA/rnhB double mutant strain, as 60% soluble and 40% insoluble protein
gene rnhB, overexpression of the enzyme in an insoluble form in different strains of Escherichia coli, overexpression of soluble enzyme with a self-cleavable intein-tag
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gene rnhC, DNA and amino acid sequence determination and analysis, overexpression of the soluble enzyme in Escherichia coli strain MIC2067(DE3)
gene rnhC, overexpression of the soluble enzyme in Escherichia coli strain MIC2067(DE3)
genetic organization of the RNase H1-related gene family, different isozymes by alternative splicing, sequence comparisons of isozymes, overexpression of His-tagged isozymes in Escherichia coli strain BL21(DE3)
-
HBV RNaseH sequences from genotype C isolate V01460 encoding residues corresponding to amino acids 688 to 844 of the HBV polymerase (reference strain ADW2, genotype A) are cloned by gene synthesis into pMAL-c5X-His with sequences encoding a His6-tag appended to the 3' end of the RNaseH gene to create pMal-HRHgtC. Sequences encoding Ala-Gly-Ala are inserted between the maltose binding protein (MBP) and HBV RNaseH sequences, and sequences encoding Gly-Ala-Gly are inserted between sequences for the RNaseH and the His6-tag. Recombinant expression of wild-type and mutant His6-tagged enzymes in Escherichia coli strain BL21(DE3)
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overexpression in strain BL21(DE3)
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overexpression of mutant enzymes in strain HB101
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overexpression of N-terminally His-tagged RNase HIII in Escherichia coli strain BL21 CodonPlus (DE3) RIPL
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overexpression of RNase HII in strain Bl21 (DE3)
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overexpression of wild-type and mutant D10A enzymes in Escherichia coli
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overexpression of wild-type and mutant enzyme
overexpression of wild-type and mutant enzymes in Escherichia coli strain HB101
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overexpression of wild-type and mutant enzymes in Escherichia coli strain MIC2067(DE3)
overexpression of wild-type and mutant enzymes in strain HB101
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overexpression of wild-type and several truncated enzymes in Escherichia coli
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overproduced in Escherichia coli
recombinant expression of C-terminally His6-tagged wild-type and mutant enzyme, without the predicted mitochondrial targeting sequence (residues 1-26), in Spodoptera frugiperda Sf9 insect cells using the baculovirus expression system
recombinant expression of His-tagged HBV ribonuclease H1 in Escherichia coli
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recombinant expression of His-tagged human ribonuclease H1 in Escherichia coli
RNase HI sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli
RNase HIIC, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3), genetic complementation of a RNase-deficient Escherichia coli strain fails due to lacking processing of the inactive precursor protein
simultaneous expression of GST-tagged RNASEH2B, and untagged RNASEH2A and RNASEH2C subunits from vector pGEX6P1 as polycistronic construct, in Escherichia coli strain Rosetta-2
type 2 enzyme, overexpression of wild-type in Escherichia coli BL21(DE3), and of selenomethionine mutant in Escherichia coli strain B834, a methionine-auxotroph BL21(DE3) derivative