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3.1.26.4: ribonuclease H

This is an abbreviated version!
For detailed information about ribonuclease H, go to the full flat file.

Word Map on EC 3.1.26.4

Reaction

Endonucleolytic cleavage to a 5'-phosphomonoester =

Synonyms

Ape-RNase HII, aRNase HII, Bh ribonuclease H, BhRNase H, CtepRNH, EcRNH, endoribonuclease H, endoribonuclease H (calf thymus), ERNH, huRNaseH1, hybrid nuclease, hybrid ribonuclease, hybridase, hybridase (ribonuclease H), KOD1, LC9-RNase H1, lmo1273, LRNase HII, More, nuclease, hybrid ribo-, nuclease, ribo-, H, P32, protein ST0753, reverse transcriptase, ribonuclease H, ribonuclease H I, ribonuclease H(42), ribonuclease H(70), ribonuclease H1, ribonuclease H2, ribonuclease H3, ribonuclease HI, ribonuclease HII, ribonuclease HIII, ribonulease H1, RNA*DNA hybrid ribonucleotidohydrolase, RNA*DNA hybrid ribonucleotiohydrolase, RNase H, RNase H type 2, RNase H(35), RNase H1, RNase H2, RNase H3, RNase HI, RNase HII, RNase HIII, RNaseH, Rnaseh1, RNASEH2A, RNH, RNH1, RNH2, rnhA, RnhB, rnhC, rnhD, Rv2228c, ST0519, STK_07530, Sto-RNase HI, T4 RNase H, Tk-RNase HII, Tk-Tk-RNase HII, TK0805, TRNH, TthRNH, Ty1 reverse transcriptase/RNase H, type 1 ribonuclease H, type 1 RNase H, type 2 ribonuclease H, type 2 RNase H, type II ribonuclease H

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.4 ribonuclease H

Specific Activity

Specific Activity on EC 3.1.26.4 - ribonuclease H

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000038
-
-
0.001
below, mutant D97A, with 50 mM Mg2+ or 10 mM Mn2+
0.0046
mutant E98A, with 10 mM Mn2+
0.0051
mutant E98A, with 10 mM Mg2+
0.03
mutant D202A, with 10 mM Mn2+
0.04
purified mutant Y46A, in presence of Mn2+, pH 8.5, 30°C
0.046
0.057
-
isozyme RNase H1beta, with Mn2+
0.06
purified mutant Q54A, in presence of Mn2+, pH 8.5, 30°C
0.068
purified mutant Q54A, in presence of Mg2+, pH 8.5, 30°C
0.07
-
isozyme RNase H1beta, with Mg2+
0.3
mutant E232A, with 10 mM Mn2+
0.31
15°C, pH 8.5, purified recombinant enzyme
0.42
mutant E232A, with 10 mM Mg2+
0.48
substrate M13 DNA/RNA hybrid, enzyme mutant W22A, pH 9.0, 30°C, in presence of 50 mM KCl
0.55
purified mutant S48A, in presence of Mn2+, pH 8.5, 30°C
0.59
purified mutant L52A, in presence of Mg2+, pH 8.5, 30°C
0.61
purified mutant S48A, in presence of Mg2+, pH 8.5, 30°C
0.75
-
recombinant soluble nontagged enzyme
1.2
purified mutant K50A, in presence of Mn2+, pH 8.5, 30°C
1.6
purified mutant K50A, in presence of Mg2+, pH 8.5, 30°C
15.2
purified wild-type enzyme, at 0.0005 mM MnCl2
2.1
-
isozyme RNase H1alpha, with Mn2+
24.2
purified mutant D134N, at 10 mM MnCl2
3.4
mutant N29K/D39G/M76V/K90N/R97G/D136H, 30°C
3.6
substrate M13 DNA/RNA hybrid, wild-type enzyme, pH 9.0, 30°C, in presence of 50 mM KCl
4.7
purified wild-type enzyme, at 1 mM MnCl2
4.9
purified wild-type enzyme, at 10 mM MnCl2
5.5
mutant N29K/D39G/M76V/K90N, 30°C
50.83
-
-
7
-
isozyme RNase H1alpha, with Mg2+
7.6
15°C, pH 8.5, purified recombinant enzyme
7.7
mutant R97G, 30°C
additional information