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Literature summary for 3.1.26.4 extracted from
- A type II ribonuclease H from Leishmania mitochondria: an enzyme essential for the growth of the parasite (2005), Mol. Biochem. Parasitol., 143, 135-145.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | the enzyme is a potential target for antileishmanial chemotherapy | Leishmania donovani |
| pharmacology | the enzyme is a potential target for antileishmanial chemotherapy | Leishmania major |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| RNase HIIC, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3), genetic complementation of a RNase-deficient Escherichia coli strain fails due to lacking processing of the inactive precursor protein | Leishmania donovani |
| RNase HIIC, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3), genetic complementation of a RNase-deficient Escherichia coli strain fails due to lacking processing of the inactive precursor protein | Leishmania major |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | the enzyme contains a mitochondrial targeting sequence with the first 40 amino acid residues at the N-terminus, the enzyme is imported into mitochondrial vesicles in vitro, the enzyme is processed after entry into mitochondria | Leishmania donovani | 5739 | - |
| mitochondrion | the enzyme contains a mitochondrial targeting sequence with the first 40 amino acid residues at the N-terminus, the enzyme is imported into mitochondrial vesicles in vitro, the enzyme is processed after entry into mitochondria | Leishmania major | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania donovani |
| 49000 | - |
x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania major |
| 53000 | - |
x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania donovani |
| 53000 | - |
x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania major |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Leishmania donovani | the enzyme is required for kinetoplast DNA replication in the mitochondrion, the RNase HIIC is essential for growth of promastigotes and amastigotes | ? | - |
? |  |
| additional information | Leishmania major | the enzyme is required for kinetoplast DNA replication in the mitochondrion, the RNase HIIC is essential for growth of promastigotes and amastigotes | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | Q8WR57 | single copy gene, 3 isozymes A, B, and C of the type II enzyme | - |
| Leishmania major | Q8WSZ0 | single copy gene, 3 isozymes A, B, and C of the type II enzyme | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the enzyme is procesed after import into mitochondria | Leishmania donovani |
| proteolytic modification | the enzyme is procesed after import into mitochondria | Leishmania major |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged RNAseHIIC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Leishmania donovani |
| recombinant His-tagged RNAseHIIC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Leishmania major |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| amastigote | - |
Leishmania donovani | - |
| amastigote | - |
Leishmania major | - |
| promastigote | - |
Leishmania donovani | - |
| promastigote | - |
Leishmania major | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is required for kinetoplast DNA replication in the mitochondrion, the RNase HIIC is essential for growth of promastigotes and amastigotes | Leishmania donovani | ? | - |
? | |
| additional information | the enzyme is required for kinetoplast DNA replication in the mitochondrion, the RNase HIIC is essential for growth of promastigotes and amastigotes | Leishmania major | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania donovani |
| ? | x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania major |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LRNase HII | - |
Leishmania donovani |
| LRNase HII | - |
Leishmania major |
| type II ribonuclease H | - |
Leishmania donovani |
| type II ribonuclease H | - |
Leishmania major |
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