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3.1.26.4: ribonuclease H

This is an abbreviated version!
For detailed information about ribonuclease H, go to the full flat file.

Word Map on EC 3.1.26.4

Reaction

Endonucleolytic cleavage to a 5'-phosphomonoester =

Synonyms

Ape-RNase HII, aRNase HII, Bh ribonuclease H, BhRNase H, CtepRNH, EcRNH, endoribonuclease H, endoribonuclease H (calf thymus), ERNH, huRNaseH1, hybrid nuclease, hybrid ribonuclease, hybridase, hybridase (ribonuclease H), KOD1, LC9-RNase H1, lmo1273, LRNase HII, More, nuclease, hybrid ribo-, nuclease, ribo-, H, P32, protein ST0753, reverse transcriptase, ribonuclease H, ribonuclease H I, ribonuclease H(42), ribonuclease H(70), ribonuclease H1, ribonuclease H2, ribonuclease H3, ribonuclease HI, ribonuclease HII, ribonuclease HIII, ribonulease H1, RNA*DNA hybrid ribonucleotidohydrolase, RNA*DNA hybrid ribonucleotiohydrolase, RNase H, RNase H type 2, RNase H(35), RNase H1, RNase H2, RNase H3, RNase HI, RNase HII, RNase HIII, RNaseH, Rnaseh1, RNASEH2A, RNH, RNH1, RNH2, rnhA, RnhB, rnhC, rnhD, Rv2228c, ST0519, STK_07530, Sto-RNase HI, T4 RNase H, Tk-RNase HII, Tk-Tk-RNase HII, TK0805, TRNH, TthRNH, Ty1 reverse transcriptase/RNase H, type 1 ribonuclease H, type 1 RNase H, type 2 ribonuclease H, type 2 RNase H, type II ribonuclease H

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.4 ribonuclease H

pH Optimum

pH Optimum on EC 3.1.26.4 - ribonuclease H

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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
the logarithm of the turnover number of the enzyme increases steeply with pH until a pH-independent region is reached close to neutrality, the pH-dependence of log 1/KM is a sigmoidal curve reaching a maximal value at higher pH, suggesting deprotonation of a residue stabilises substrate binding
7.4 - 8.8
-
in presence of 0.5 mM Mn2+, enzyme form HA1
7.5 - 10
Mg-dependent hydrolysis activity by Tm-RNase H2 gradually increases from pH 7.5 to 10 without reaching a maximum
7.5 - 8
-
Tris buffer or potassium phosphate buffer
7.5 - 9.1
-
-
7.6
-
in presence of 0.5 mM Mn2+, enzyme form HA2, HB1 and HB2
7.7
-
in presence of 40 mM Mg2+, enzyme form HB1
7.8 - 7.9
-
assay at
8 - 8.5
-
in presence of 10 mM Mg2+
8 - 9
-
-
8.3
-
in presence of 5 mM Mg2+, enzyme form HA1
8.4
-
enzyme form H-1
8.5 - 9
8.5 - 9.5
-
cleavage of M13 DNA/RNA hybrid
8.6
-
in presence of 5 mM Mg2+, enzyme form HA2
9.5
-
Mg2+-dependent enzyme
9.8
-
above
additional information