Protein Variants | Comment | Organism |
---|---|---|
D125N | mutation results in loss of dsRNase activity but not RNase H activity | Sulfurisphaera tokodaii |
D76N | no RNase H activity is detected | Sulfurisphaera tokodaii |
D7N | enzyme loses RNase H activity and dsRNase activity in the presence of MnCl2 | Sulfurisphaera tokodaii |
E52Q | no RNase H activity is detected | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions. The enzyme cleaves an RNA/RNA duplex in the presence of Mn2+ or Co2+ | Sulfurisphaera tokodaii | |
Mg2+ | the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions | Sulfurisphaera tokodaii | |
Mn2+ | the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions. The enzyme cleaves an RNA/RNA duplex in the presence of Mn2+ or Co2+ | Sulfurisphaera tokodaii | |
Ni2+ | the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13000 | - |
gel filtration | Sulfurisphaera tokodaii |
16812 | - |
1 * 16812, calculated from sequence | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | F9VN79 | - |
- |
Sulfurisphaera tokodaii 7 | F9VN79 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA + H2O | the enzyme cleaves the RNA strand of an RNA/DNA hybrid or an RNA/RNA duplex in the presence of Mn2+ or Co2+ | Sulfurisphaera tokodaii | ? | - |
? | |
RNA + H2O | the enzyme cleaves the RNA strand of an RNA/DNA hybrid or an RNA/RNA duplex in the presence of Mn2+ or Co2+ | Sulfurisphaera tokodaii 7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 16812, calculated from sequence | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
protein ST0753 | - |
Sulfurisphaera tokodaii |
RNase HI | - |
Sulfurisphaera tokodaii |
type 1 RNase H | - |
Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
the standard cleavage reaction is performed at a pH of 8.5 in the presence of 1 mM MgCl2 or MnCl2, because at a higher pH range, the solubility of divalent metal ions decreases and RNA/DNA substrates might become destabilized | Sulfurisphaera tokodaii |
10 | - |
the cleavage activity increases exponentially as the pH increases from 6 to 10 | Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the cleavage activity increases exponentially as the pH increases from 6 to 10 | Sulfurisphaera tokodaii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sulfurisphaera tokodaii | calculated from sequence | - |
9.5 |