Information on EC 3.1.26.4 - ribonuclease H

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.26.4
-
RECOMMENDED NAME
GeneOntology No.
ribonuclease H
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Endonucleolytic cleavage to 5'-phosphomonoester
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
CAS REGISTRY NUMBER
COMMENTARY hide
9050-76-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
Aeropyrum pernix DSM 11879
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
different splicing variants
-
-
Manually annotated by BRENDA team
strain GD-2
-
-
Manually annotated by BRENDA team
one Mn2+-dependent RNase H and one Mg2+-dependent RNase H
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
single copy gene, 3 isozymes A, B, and C of the type II enzyme
SwissProt
Manually annotated by BRENDA team
single copy gene, 3 isozymes A, B, and C of the type II enzyme
SwissProt
Manually annotated by BRENDA team
4 enzyme form: HA1, HA2, HB1 and HB2
-
-
Manually annotated by BRENDA team
type 2 enzyme
-
-
Manually annotated by BRENDA team
bifunctional protein, the N-terminal domain is homologous with prokaryotic and eukaryotic RNase H domains and the C-terminal domain with alpha-ribazole phosphatase CobC
UniProt
Manually annotated by BRENDA team
bifunctional protein, the N-terminal domain is homologous with prokaryotic and eukaryotic RNase H domains and the C-terminal domain with alpha-ribazole phosphatase CobC
UniProt
Manually annotated by BRENDA team
gene PAB0352
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain SIB1, psychrotrophic bacterium, gene rnhB
SwissProt
Manually annotated by BRENDA team
strain SIB1, psychrotrophic bacterium, gene rnhB
SwissProt
Manually annotated by BRENDA team
strain GI, enzyme form: H-1, H-2 and H-3
-
-
Manually annotated by BRENDA team
strain GI, enzyme form: H-1, H-2 and H-3
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
type I enzyme
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
clone VP62
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
12 base pair RNA-DNA hybrid + H2O
ribonucleotide 5'-phosphomonoester + ?
show the reaction diagram
12 basepair DNA-DNA duplex + H2O
?
show the reaction diagram
oligomeric substrate, cleavage at multiple sites, product identification
-
-
?
12bp-RNA-DNA hybrid + H2O
?
show the reaction diagram
29 basepair DNA-RNA-DNA/DNA + H2O
?
show the reaction diagram
oligomeric substrate, cleavage mainly in the middle of the tetraribonucleotide, product identification
-
-
?
35 bp DNA-RNA-DNA/DNA chimeric hybrid + H2O
?
show the reaction diagram
5'-(6-carboxy-fluorescein)-cggagaugacgg-3'/5'-CCGTCTCTCCG-3' + H2O
?
show the reaction diagram
D13-R4-D12-D29 hybrid + H2O
?
show the reaction diagram
-
-
-
?
D14R1D3:DNA18 + H2O
?
show the reaction diagram
-
-
-
?
DNA*DNA + H2O
?
show the reaction diagram
-
5'-to 3'-exonuclease activity, degradation of DNA*DNA duplexes
-
-
?
DNA-(1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid + H2O
?
show the reaction diagram
-
-
-
-
?
DNA-(2'-alkoxy-1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid + H2O
?
show the reaction diagram
-
-
-
-
?
DNA-(aza-ENA-T)-antisense-RNA hybrid + H2O
?
show the reaction diagram
-
-
-
-
?
DNA-(azetidine-T)-antisense-RNA hybrid + H2O
?
show the reaction diagram
-
-
-
-
?
DNA-(oxetane-T)-antisense-RNA hybrid + H2O
?
show the reaction diagram
-
-
-
-
?
DNA-2'-methoxyethoxy RNA hybrid
?
show the reaction diagram
-
chimeric substrates containing a central DNA region with flanking northern-biased 2'-methoxyethyl nucleotides hybridized to complementary RNA, enhanced cleavage rates are observed for the eastern-biased 2'-ara-fluorothymidine and bulge inducing N-methylthymidine modifications positioned at the 5'-DNA/3'-MOE junction as well as the southern-biased 2'-methylthiothymidine and conformationally flexible tetrafluoroindole modifications positioned at the 5'-MOE/3'-DNA junction, overview
-
-
-
DNA-2'-methoxyethoxy-antisense RNA hybrid + H2O
?
show the reaction diagram
-
2'-methoxyethoxy nucleotides, positioned at the 3' and 5' poles, into the antisense oligodeoxyribonucleotide of the heteroduplex to alter the helical geometry of the substrate
-
-
?
DNA-rN1-DNA/DNA + H2O
?
show the reaction diagram
-
specific cleavage by RNase HII at the 5'-side of the ribonucleotide, cleavage efficiencies of the perfectly matched DNA-rN1-DNA/DNA duplexes are higher than those carrying a mismatched ribonucleotide
-
-
?
DNA-RNA duplex + H2O
?
show the reaction diagram
DNA-RNA hybrid + H2O
?
show the reaction diagram
DNA-RNA hybrid + H2O
DNA + 5'-phosphonucleotides
show the reaction diagram
DNA-RNA hybrid + H2O
ssDNA + 5'-phosphomonoester oligonucleotides
show the reaction diagram
-
in the pause of minus strang synthesis, RNAse H degrades the RNA template, with the exception of the polypurine tract sequence, immediately upstream of U3, which serves as a primer for plus-strand synthesis
-
-
?
DNA-RNA hybrid duplex + H2O
oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
show the reaction diagram
DNA-RNA-DNA hybrid + H2O
?
show the reaction diagram
-
specific hydrolysis of the RNA strand of the hybrid
-
-
?
DNA-RNA-DNA/DNA hybrid + H2O
?
show the reaction diagram
a duplex containing a (5')RNA-DNA(3') junction with one, three, or six ribonucleotides, i.e. DNA5-RNA1-DNA6/DNA12, DNA3-RNA3-DNA6/DNA12, and RNA6-DNA6/DNA12, and a substrate with a (5')DNA-RNA(3') junction, DNA5-RNA7/DNA12
-
-
?
DNA12-RNA1-DNA27/DNA40 hybrid + H2O
?
show the reaction diagram
-
enzyme cleaves RNA20/DNA20 hybrid and DNA12-RNA1-DNA27/DNA40 hybrid substrates with similar efficiency
-
?
dsDNA oligonucleotide with a single ribose + H2O
dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide
show the reaction diagram
dsDNA oligonucleotide with a stretch of ribonucleotides + H2O
dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide
show the reaction diagram
-
enzyme excises misincorporated ribonucleotides in DNA
-
-
?
dsDNA oligonucleotides with a single ribose + H2O
dsDNA oligonucleotides with 1 nt gap + 5'-monophosphate ribonucleotide
show the reaction diagram
-
preferred substrate, enzyme excises misincorporated ribonucleotides in DNA, enzyme places the first 5' nick, while the second 3' cut is made by Rad27p
-
-
?
M13 DNA-RNA hybrid + H2O
?
show the reaction diagram
M13 DNA/RNA hybrid + H2O
?
show the reaction diagram
peptide nucleic acid - 2'-deoxy 2'-fluoroarabinonucleic acid hybrid + H2O
?
show the reaction diagram
-
chimeric oligomers possessing a single central peptide nucleic acid insert are capable of forming hybrid duplexes with complementary RNA, although with diminished thermal stability in comparison to the unmodified oligomers
-
-
?
peptide nucleic acid - DNA + H2O
?
show the reaction diagram
-
chimeric oligomers possessing a single central peptide nucleic acid insert are capable of forming hybrid duplexes with complementary RNA, although with diminished thermal stability in comparison to the unmodified oligomers
-
-
?
poly(rA)/poly(dT) + H2O
?
show the reaction diagram
-
-
-
?
poly-rA/poly-dT + H2O
?
show the reaction diagram
-
products are short oligonucleotides with very few intermediate-sized oligonucleotides
-
?
polyA*dT36 hybrid + H2O
?
show the reaction diagram
-
-
-
-
?
PPT-RNA + H2O
?
show the reaction diagram
RNA + H2O
?
show the reaction diagram
RNA*2'F-ANA-DNA hybrid + H2O
?
show the reaction diagram
RNA*antisense-DNA hybrid + H2O
?
show the reaction diagram
RNA*DNA hybrid + H2O
5'-phospho-3'-hydroxyoligonucleotides
show the reaction diagram
-
specifically degrades the RNA moiety
-
-
?
RNA*DNA hybrid + H2O
?
show the reaction diagram
RNA*DNA hybrid + H2O
DNA + 5'-phosphonucleotides
show the reaction diagram
-
5'-to 3'-exonuclease activity, degradation of RNA*DNA duplexes
-
-
?
RNA-DNA duplex + H2O
?
show the reaction diagram
RNA-DNA heteroduplex + H2O
ribonucleotide 5'-phosphomonoester + ?
show the reaction diagram
-
-
-
-
?
RNA-DNA hybrid + H2O
?
show the reaction diagram
RNA-DNA hybrid + H2O
ribonucleotide 5'-phosphomonoester
show the reaction diagram
RNA-DNA hybrid + H2O
ribonucleotide 5'-phosphomonoester + ?
show the reaction diagram
RNA-DNA hybrid HTS-1 + H2O
?
show the reaction diagram
5'-GAUCUGAGCCUGGGAGCU-fluorescein-3' annealed to 5'-Dabcyl-AGCTCCCAGGCTCAGATC-3'
-
-
?
RNA-DNA hybrid HTS-2 + H2O
?
show the reaction diagram
5'-CUGGUUAGACCAGAUCUGAGCCUGGGAGCU-fluorescein-3' annealed to 5'-Dabcyl-AGCTCCCAGGCTCAGATC-3'
-
-
?
RNA-DNA single-stranded chimera + H2O
?
show the reaction diagram
-
-
-
?
RNA-DNA*DNA hybrid + H2O
8mer oligonucleotide
show the reaction diagram
7 M urea-denatured 18mer, no activity with untreated hybrid, specific removal of the RNA portion of the duplex
-
-
?
RNA-DNA*DNA hybrid + H2O
DNA*DNA + 5'-phosphomononucleotides
show the reaction diagram
RNA-DNA*DNA hybrid + H2O
oligonucleotides
show the reaction diagram
7 M urea-denatured 37mer, no activity with untreated hybrid, specific removal of the RNA portion of the duplex
a 20mer, a 10mer, a 9mer, and a 7mer
-
?
RNA-DNA/DNA hybrid + H2O
?
show the reaction diagram
-
PabRNase HII acts as a specific endonuclease on RNA-DNA/DNA duplexes. Specific cleavage, one nucleotide upstream of the RNA-DNA junction, occurs on a substrate in which RNA initiators is fully annealed to the cDNA template. Additionally, PabRNase HII cleaves a single ribonucleotide embedded in a double-stranded DNA
-
-
?
RNA-RNA duplex + H2O
?
show the reaction diagram
RNA/DNA hybrid + H2O
?
show the reaction diagram
RNA18:DNA18 + H2O
?
show the reaction diagram
-
-
-
?
RNA20/DNA20 hybrid + H2O
?
show the reaction diagram
-
enzyme cleaves RNA20/DNA20 hybrid and DNA12-RNA1-DNA27/DNA40 hybrid substrates with similar efficiency
-
?
single-stranded RNA + H2O
?
show the reaction diagram
-
isoenzyme C-1 and C-2 specifically act on the RNA moiety of RNA-DNA hybrid, isoenzyme C-3 degrades single-stranded RNA as well as the RNA of hybrids
-
-
?
ssDNA-dsDNA + H2O
?
show the reaction diagram
-
5'-to 3'-exonuclease activity, exonuclease activity removing short oligonucleotides of 3-30 nucleotides from adjacent DNA
-
-
?
ssRNA + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
12 base pair RNA-DNA hybrid + H2O
ribonucleotide 5'-phosphomonoester + ?
show the reaction diagram
DNA-RNA duplex + H2O
?
show the reaction diagram
DNA-RNA hybrid + H2O
DNA + 5'-phosphonucleotides
show the reaction diagram
DNA-RNA hybrid + H2O
ssDNA + 5'-phosphomonoester oligonucleotides
show the reaction diagram
-
in the pause of minus strang synthesis, RNAse H degrades the RNA template, with the exception of the polypurine tract sequence, immediately upstream of U3, which serves as a primer for plus-strand synthesis
-
-
?
DNA-RNA hybrid duplex + H2O
oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
show the reaction diagram
-
-
-
?
RNA*DNA hybrid + H2O
?
show the reaction diagram
RNA-DNA heteroduplex + H2O
ribonucleotide 5'-phosphomonoester + ?
show the reaction diagram
-
-
-
-
?
RNA-DNA hybrid + H2O
?
show the reaction diagram
RNA-DNA hybrid + H2O
ribonucleotide 5'-phosphomonoester
show the reaction diagram
RNA-DNA hybrid + H2O
ribonucleotide 5'-phosphomonoester + ?
show the reaction diagram
RNA-DNA*DNA hybrid + H2O
DNA*DNA + 5'-phosphomononucleotides
show the reaction diagram
RNA-DNA/DNA hybrid + H2O
?
show the reaction diagram
-
PabRNase HII acts as a specific endonuclease on RNA-DNA/DNA duplexes. Specific cleavage, one nucleotide upstream of the RNA-DNA junction, occurs on a substrate in which RNA initiators is fully annealed to the cDNA template. Additionally, PabRNase HII cleaves a single ribonucleotide embedded in a double-stranded DNA
-
-
?
ssDNA-dsDNA + H2O
?
show the reaction diagram
-
5'-to 3'-exonuclease activity, exonuclease activity removing short oligonucleotides of 3-30 nucleotides from adjacent DNA
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co3+
strictly metal-dependent nuclease. It exhibits activity in the presence of Mg2+, Mn2+, Co2+ or Ni2+, whereas no activity is observed in the absence of these metal ions. Little activity is detected in the presence of other metals including Co3+, Cu2+, Zn2+, and Ca2+
Cu2+
strictly metal-dependent nuclease. It exhibits activity in the presence of Mg2+, Mn2+, Co2+ or Ni2+, whereas no activity is observed in the absence of these metal ions. Little activity is detected in the presence of other metals including Co3+, Cu2+, Zn2+, and Ca2+
Na+
the enzyme exhibits the highest activity in the presence of 100 mm NaCl
NH4Cl
-
enzyme form H2 is mostly inactive at low salt and requires 100-200 mM concentration for maximal activity. NH4Cl is more efficient than NaCl
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,3R,4R,5R,8S)-8-benzyloxy-1-benzyloxymethyl-5-benzyloxyamino-3-(thymin-1-yl)-2-oxa-bicyclo[3.2.1]octane
-
-
(1R,3R,4R,5R,8S)-8-benzyloxy-1-benzyloxymethyl-5-trifluoroacetamino-3-(thymin-1-yl)-2-oxa-bicyclo[3.2.1]octane
-
-
(1R,3R,4R,5S,8S)-8-benzyloxy-1-benzyloxymethyl-5-((methylthio)thiocarbonyl)oxy-3-(thymin-1-yl)-2-oxa-bicyclo[3.2.1]octane
-
-
(1R,3R,4R,5S,8S)-8-benzyloxy-1-benzyloxymethyl-5-(4-methylbenzoyl)-3-(thymin-1-yl)-2-oxa-bicyclo[3.2.1]octane
-
-
(1R,3R,4R,5S,8S)-8-benzyloxy-1-benzyloxymethyl-5-hydroxy-3-(thymin-1-yl)-2-oxa-bicyclo[3.2.1]octane (20a) and (1R,3R,4R,5R,8S)-8-benzyloxy-1-benzyloxymethyl-5-hydroxy-3-(thymin-1-yl)-2-oxabicyclo[3.2.1]octane
-
-
(1R,3R,4R,5S,8S)-8-benzyloxy-1-benzyloxymethyl-5-methoxalyloxy-5-methyl-3-(thymin-1-yl)-2-oxa-bicyclo[3.2.1]octane
-
-
(1R,3R,4R,8S)-8-benzyloxy-1-benzyloxymethyl-3-(thymin-1-yl)-2-oxa-bicyclo[3.2.1]octane
-
-
(1R,3R,4R,8S)-8-benzyloxy-1-benzyloxymethyl-5-one-3-(thymin-1-yl)-2-oxa-bicyclo[3.2.1]octane
-
-
(NH4)2SO4
1,10-phenanthroline
-
inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme
1-(2-O-acetyl-3,5-O-benzyl-4-C-cyanoethyl-beta-D-ribofuranosyl)-thymine
-
-
1-(3,5-O-benzyl-2-O-phenoxythiocarbonyl-4-C-propionaldehyde-beta-D-ribofuranosyl)thymineO-benzyloxime
-
-
1-(3,5-O-benzyl-4-C-cyanoethyl-2-O-hydroxyl-beta-D-ribofuranosyl)-thymine
-
-
1-(3,5-O-benzyl-4-C-propionaldehyde-2-O-hydroxyl-beta-D-ribofuranosyl)thymine O-benzyl oxime
-
-
2-(2,3-dimethylphenyl)-6-fluoro-1,2-benzothiazol-3(2H)-one
-
-
2-(2,5-dimethylphenyl)-6-fluoro-1,2-benzothiazol-3(2H)-one
-
-
2-(4,6-dimethyl-3-oxo-1,2-benzothiazol-2(3H)-yl)-N-propylacetamide
-
-
2-(4-chlorophenyl)-6-fluoro-1,2-benzothiazol-3(2H)-one
-
-
2-(6-fluoro-3-oxo-1,2-benzothiazol-2(3H)-yl)-N-(4-methylphenyl)acetamide
-
-
2-phenyl-1,2-benzothiazol-3(2H)-one
-
-
2-[(cyclopentylcarbonyl)amino]-4-ethyl-5-methylthiophene-3-carboxamide
-
-
3,5-di-O-benzyl-4-C-cyanoethyl-1,2-O-isopropylidene-alpha-D-ribofuranose
-
-
4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one
-
-
5-nitrofuran-2-carboxylic acid [[4-(4-bromophenyl)-thiazol-2-yl]-(tetrahydrofuran-2-ylmethyl)-carbamoyl]-methyl ester
-
derivative of 5-nitrofuran-2-carboxylic acid carbamoyl methyl ester. 20-25 microM effectively inhibit HIV-1 replication
6-(naphthalen-2-yl)-3-(pyridin-3-yl)[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole
-
-
6-fluoro-2-(2-methylphenyl)-1,2-benzothiazol-3(2H)-one
-
-
6-fluoro-2-(4-methylphenyl)-1,2-benzothiazol-3(2H)-one
-
-
7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione
YLC2-155
-
acetonitrile
-
20%, 50% loss of activity
alpha-thujaplicin
antisense oligodeoxynucleotides
-
directed against RNA polymerase II, replication protein A, and Ha-ras, determination of response in expression levels of the enzyme type 1 and 2, overview
-
beta-thujaplicin
beta-thujaplicinol
Cu2+
-
in presence of Mg2+, inhibition
Dextran
diphosphate
-
inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme
DNA
-
single-stranded or double-stranded, very strong
DTT
-
-
EDTA
-
-
Ethidium bromide
-
-
Fe2+
-
in presence of Mg2+, inhibition
gamma-thujaplicin
Hg2+
-
-
manicol
Mg2+
-
Mg2+ is inhibitory at concentrations above 10 mM
N-(5-benzyl-1,3,4-thiadiazol-2-yl)-2-ethylhexanamide
-
-
N-benzyl-2-(6-fluoro-3-oxo-1,2-benzothiazol-2(3H)-yl)acetamide
-
-
N-cyclopentyl-2-(4,6-dimethyl-3-oxo[1,2]thiazolo[5,4-b]pyridin-2(3H)-yl)acetamide
-
-
N-cyclopropyl-1-methyl-3-oxo-1,3-dihydro-2,1-benzothiazole-5-sulfonamide
-
-
N-ethylmaleimide
-
inhibits wild-type enzyme and deletion mutant H1[DELTA1-73]
NaF
-
inhibition of Mg2+-dependent enzyme
NH4Cl
-
activity of enzyme form H1 decreases rapidly above 50 mM and becomes nearly abolished at 150 mM
nootkatin
Nucleic acids
-
enzyme form H1 is more susceptible to inhibition than enzyme form H2
-
poly(rA)
-
noncompetitive
-
poly(rArU)
-
noncompetitive
-
poly(rCdG)
-
uncompetitive
-
poly(rIdC)
-
competitive
-
Polyribonucleotides
-
slight
reovirus RNA
-
competitive
-
rifampicin
rifampicin AF/013
-
-
S-adenosylhomocysteine
-
-
S-adenosylmethionine
SDS
-
0.002%, 50% loss of activity
spermidine
-
inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme
spermine
-
inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme
trihydroxy benzoyl biphenyl carboxylate hydrazone
BHMP07
-
trihydroxybenzoyl naphthyl hydrazone
-
-
tropolone
Zn2+
-
in presence of Mg2+, inhibition
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
2-mercaptoethanol
dithiothreitol
-
optimal concentration is 1 mM
Sulfhydryl reagent
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000361
5'-(6-carboxy-fluorescein)-cggagaugacgg-3'/5'-CCGTCTCTCCG-3'
pH 9.0, 30C
-
0.000027 - 0.00003
D14R1D3:DNA18
0.000122
DNA-(1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid
-
pH 7.5, 21C
-
0.000242
DNA-(2'-alkoxy-1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid
-
pH 7.5, 21C
-
0.000532
DNA-(aza-ENA-T)-antisense-RNA hybrid
-
pH 7.5, 21C
-
0.000236
DNA-(azetidine-T)-antisense-RNA hybrid
-
pH 7.5, 21C
-
0.000083
DNA-(oxetane-T)-antisense-RNA hybrid
-
pH 7.5, 21C
-
0.000064
DNA-RNA hybrid
-
pH 7.5, 21C
-
0.0011 - 0.0078
DNA-RNA-DNA hybrid
0.0014
DNA/RNA hybrid
-
obtained by transcription of calf thymus DNA
-
0.071 - 0.26
M13 DNA-RNA hybrid
0.0005 - 0.0011
M13 DNA/RNA
0.00039 - 0.0014
M13 DNA/RNA hybrid
0.0005 - 0.0019
poly(rAdT)
0.012
polyA*dT36
-
pH 8.0, 500 mM NaCl, wild-type enzyme
-
0.00009 - 0.00837
RNA*DNA hybrid
0.00012 - 0.0002
RNA-DNA duplex
0.000097 - 0.000106
RNA-DNA heteroduplex
0.0011 - 0.0028
RNA-DNA hybrid
0.00006 - 0.0036
RNA-DNA*DNA hybrid
0.0005
RNA-DNA/DNA hybrid
-
pH 8.0, 37C, purified recombinant His-tagged enzyme
-
0.0013
RNA-RNA duplex
wild-type, 50C, presence of Mn2+
-
0.000143 - 0.000158
RNA18:DNA18
additional information
additional information