3.1.26.4: ribonuclease H

This is an abbreviated version!
For detailed information about ribonuclease H, go to the full flat file.

Reaction

Endonucleolytic cleavage to a 5'-phosphomonoester =

Synonyms

Ape-RNase HII, aRNase HII, Bh ribonuclease H, BhRNase H, CtepRNH, EcRNH, endoribonuclease H, endoribonuclease H (calf thymus), ERNH, huRNaseH1, hybrid nuclease, hybrid ribonuclease, hybridase, hybridase (ribonuclease H), KOD1, LC9-RNase H1, lmo1273, LRNase HII, More, nuclease, hybrid ribo-, nuclease, ribo-, H, P32, protein ST0753, reverse transcriptase, ribonuclease H, ribonuclease H I, ribonuclease H(42), ribonuclease H(70), ribonuclease H1, ribonuclease H2, ribonuclease H3, ribonuclease HI, ribonuclease HII, ribonuclease HIII, ribonulease H1, RNA*DNA hybrid ribonucleotidohydrolase, RNA*DNA hybrid ribonucleotiohydrolase, RNase H, RNase H type 2, RNase H(35), RNase H1, RNase H2, RNase H3, RNase HI, RNase HII, RNase HIII, RNaseH, Rnaseh1, RNASEH2A, RNH, RNH1, RNH2, rnhA, RnhB, rnhC, rnhD, Rv2228c, ST0519, STK_07530, Sto-RNase HI, T4 RNase H, Tk-RNase HII, Tk-Tk-RNase HII, TK0805, TRNH, TthRNH, Ty1 reverse transcriptase/RNase H, type 1 ribonuclease H, type 1 RNase H, type 2 ribonuclease H, type 2 RNase H, type II ribonuclease H

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.26 Endoribonucleases producing 5'-phosphomonoesters

                3.1.26.4 ribonuclease H

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Results	in table
16	Activating Compound
117230	AA Sequence
12	Application
1	CAS Registry Number
64	Cloned(Commentary)
35	Crystallization (Commentary)
18003	Disease/ Diagnostics
79	General Information
10	General Stability
24	IC50 Value
171	Inhibitors
10	kcat/KM [mM/s]
2	Ki Value [mM]
87	KM Value [mM]
39	Localization
233	Metals/Ions
71	Molecular Weight [Da]
69	Natural Substrates/ Products (Substrates)
441	Organism
470	PDB ID
83	pH Optimum
16	pH Range
2	pH Stability
2	pI Value
2	Posttranslational Modification
280	Protein Variants
76	Purification (Commentary)
20	Reaction
2	Reaction Type
219	Reference
6	Renatured (Commentary)
58	Source Tissue
67	Specific Activity [micromol/min/mg]
4	Storage Stability
332	Substrates and Products (Substrate)
61	Subunits
315	Synonyms
53	Temperature Optimum [°C]
6	Temperature Range [°C]
41	Temperature Stability [°C]
49	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.1.26.4 - ribonuclease H

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, 0.025 mg enzyme per ml, in 0.1 M Tris-HCl, pH 7.5, 0.01 MgCl2, 20 mM urea, 1 mM DTT, 0.1% Triton X-100, 50% v/v glycerol, stable for several weeks

Escherichia coli

-

134080

-20°C, 25 mM Tris-HCl, pH 7.5, 30 mM NaCl, 0.5 mM EDTA, 5 mM 2-mercaptoethanol, 50% glycerol, stable

Escherichia coli

-

663628

-20°C, 50% v/v glycerol, stable for several months

Rattus norvegicus

-

134387

-70°C, complete loss of activity after a few days

Mesocricetus auratus

-

134395