EC Number   |
pH Optimum Minimum |
pH Optimum Maximum |
Reference |
|---|
   3.1.26.4 | -999 |
- |
folding/unfolding and metal binding at different pH values |
657310 |
| 3.1.26.4 | -999 |
- |
the type 2 RNase H is an Mg2+- and alkaline pH-dependent enzyme |
715367 |
| 3.1.26.4 | 6.5 |
- |
the logarithm of the turnover number of the enzyme increases steeply with pH until a pH-independent region is reached close to neutrality, the pH-dependence of log 1/KM is a sigmoidal curve reaching a maximal value at higher pH, suggesting deprotonation of a residue stabilises substrate binding |
681431 |
| 3.1.26.4 | 7.4 |
8.8 |
in presence of 0.5 mM Mn2+, enzyme form HA1 |
134395 |
| 3.1.26.4 | 7.5 |
- |
assay at |
655447, 656012, 656122, 657193, 665555, 678280, 682020, 682021, 716001 |
| 3.1.26.4 | 7.5 |
- |
enzyme form H-2 |
134398 |
| 3.1.26.4 | 7.5 |
- |
in Hepes buffer |
729704 |
| 3.1.26.4 | 7.5 |
- |
in presence of 40 mM Mg2+, enzyme form HB2 |
134395 |
| 3.1.26.4 | 7.5 |
8 |
Tris buffer or potassium phosphate buffer |
134397 |
| 3.1.26.4 | 7.5 |
9.1 |
- |
134376 |
