Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | enzyme form HA1, HA2, and HB1 | Mesocricetus auratus | |
NEM | 2 mM or above | Mesocricetus auratus | |
S-adenosylmethionine | 2 mM or above | Mesocricetus auratus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | stimulates enzyme HB2 | Mesocricetus auratus | |
Mg2+ | optimal concentrations for the 4 enzyme forms at pH 7.6 and at pH 8.3 | Mesocricetus auratus | |
Mn2+ | optimal concentrations for the 4 enzyme forms at pH 7.6 and at pH 8.3 | Mesocricetus auratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | - |
4 enzyme form: HA1, HA2, HB1 and HB2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | BHK-21/C13 | Mesocricetus auratus | - |
Storage Stability | Organism |
---|---|
-70°C, complete loss of activity after a few days | Mesocricetus auratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA hybrid + H2O | - |
Mesocricetus auratus | ribonucleotide 5'-phosphomonoester | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8.8 | in presence of 0.5 mM Mn2+, enzyme form HA1 | Mesocricetus auratus |
7.5 | - |
in presence of 40 mM Mg2+, enzyme form HB2 | Mesocricetus auratus |
7.6 | - |
in presence of 0.5 mM Mn2+, enzyme form HA2, HB1 and HB2 | Mesocricetus auratus |
7.7 | - |
in presence of 40 mM Mg2+, enzyme form HB1 | Mesocricetus auratus |
8.3 | - |
in presence of 5 mM Mg2+, enzyme form HA1 | Mesocricetus auratus |
8.6 | - |
in presence of 5 mM Mg2+, enzyme form HA2 | Mesocricetus auratus |