Cloned (Comment) | Organism |
---|---|
expression of enzyme type 1 and 2 as GFP-fusion proteins in all 6 cell lines, transfection of cell lines with antisense oligodeoxynucleotides directed against RNA polymerase II, replication protein A, and Ha-ras, determination of response in expression levels of the enzyme type 1 and 2, overview | Homo sapiens |
General Stability | Organism |
---|---|
partially purified enzyme extract is labile against freezing and dilution, addition of 45% glycerol | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antisense oligodeoxynucleotides | directed against RNA polymerase II, replication protein A, and Ha-ras, determination of response in expression levels of the enzyme type 1 and 2, overview | Homo sapiens | |
additional information | presence of intrinsic cell-type specific factors affecting the activity and localization of type 2 enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | enzyme type 1 of all cell lines and enzyme type 2 of 15PC3 are distributed throughout the whole cell, presence of intrinsic cell-type specific factors affecting the activity and localization of type 2 enzyme | Homo sapiens | - |
- |
nucleus | type 2 enzyme, restricted to, except in cell line 15PC3 | Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Homo sapiens | |
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | presence of intrinsic cell-type specific factors affecting the activity and localization of type 2 enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
type 1 and type 2 enzymes | - |
Purification (Comment) | Organism |
---|---|
partially | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
15PC3 cell | prostate cancer cell line, high level in enzyme type 1 and 2 activity | Homo sapiens | - |
HEK-293 cell | embryonal kidney cell line, high level in enzyme type 1 activity, low level in enzyme type 2 activity | Homo sapiens | - |
HeLa cell | cervical carcinoma cell line, high level in enzyme type 1 activity, moderate level in enzyme type 2 activity | Homo sapiens | - |
HTB-82 cell | rhabdomyosarcoma cell line, low level in enzyme type 1 and 2 activity | Homo sapiens | - |
MiaPaCa-2 cell | pancreatic carcinoma cell line, low level in enzyme type 1 activity, high level in enzyme type 2 activity | Homo sapiens | - |
T-24 cell | bladder carcinoma cell line, low level in enzyme type 1 activity, moderate level in enzyme type 2 activity | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
determination of RNA levels | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | presence of intrinsic cell-type specific factors affecting the activity and localization of type 2 enzyme | Homo sapiens | ? | - |
? | |
RNA*DNA hybrid + H2O | specific for the RNA moiety | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease H | - |
Homo sapiens |
RNase H | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |