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Literature summary for 3.1.26.4 extracted from

  • Liang, R.; Liu, X.; Pei, D.; Liu, J.
    Biochemical characterization and functional complementation of ribonuclease HII and ribonuclease HIII from Chlamydophila pneumoniae AR39 (2007), Microbiology, 153, 787-793.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of RNase HII and RNase HIII in the enzyme-deficient Escherichia coli strain DY329, complementation assay, Chlamydophila pneumoniae RNase HII can complement both Escherichia coli RNase HII and RNase HI, but Chlamydophila pneumoniae RNase HIII can only complement the latter Chlamydia pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information Chlamydophila pneumoniae RNase HII can complement both Escherichia coli RNase HII and RNase HI, but Chlamydophila pneumoniae RNase HIII can only complement the latter Chlamydia pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Chlamydia pneumoniae

Organism

Organism UniProt Comment Textmining
Chlamydia pneumoniae
-
RNases HII and HIII, encoded in ORFs CP0654 and CP0782
-
Chlamydia pneumoniae AR39
-
RNases HII and HIII, encoded in ORFs CP0654 and CP0782
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12bp-RNA-DNA hybrid + H2O RNase HII cleaves the 12 bp RNA-DNA substrate at multiple sites, but RNase HIII at only one site Chlamydia pneumoniae ?
-
?
12bp-RNA-DNA hybrid + H2O RNase HII cleaves the 12 bp RNA-DNA substrate at multiple sites, but RNase HIII at only one site Chlamydia pneumoniae AR39 ?
-
?
35 bp DNA-RNA-DNA/DNA chimeric hybrid + H2O
-
Chlamydia pneumoniae ?
-
?
35 bp DNA-RNA-DNA/DNA chimeric hybrid + H2O
-
Chlamydia pneumoniae AR39 ?
-
?
additional information substrate specificity and involved active site residues of RNases HII and HIII, overview Chlamydia pneumoniae ?
-
?
additional information substrate specificity and involved active site residues of RNases HII and HIII, overview Chlamydia pneumoniae AR39 ?
-
?

Synonyms

Synonyms Comment Organism
RNase HII
-
Chlamydia pneumoniae
RNase HIII
-
Chlamydia pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Chlamydia pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
neither Chlamydophila pneumoniae RNase HII nor Chlamydophila pneumoniae RNase HIII is thermostable Chlamydia pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10
-
Chlamydia pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
5 12
-
Chlamydia pneumoniae