3.1.26.4: ribonuclease H

This is an abbreviated version!
For detailed information about ribonuclease H, go to the full flat file.

Reaction

Endonucleolytic cleavage to a 5'-phosphomonoester =

Synonyms

Ape-RNase HII, aRNase HII, Bh ribonuclease H, BhRNase H, CtepRNH, EcRNH, endoribonuclease H, endoribonuclease H (calf thymus), ERNH, huRNaseH1, hybrid nuclease, hybrid ribonuclease, hybridase, hybridase (ribonuclease H), KOD1, LC9-RNase H1, lmo1273, LRNase HII, More, nuclease, hybrid ribo-, nuclease, ribo-, H, P32, protein ST0753, reverse transcriptase, ribonuclease H, ribonuclease H I, ribonuclease H(42), ribonuclease H(70), ribonuclease H1, ribonuclease H2, ribonuclease H3, ribonuclease HI, ribonuclease HII, ribonuclease HIII, ribonulease H1, RNA*DNA hybrid ribonucleotidohydrolase, RNA*DNA hybrid ribonucleotiohydrolase, RNase H, RNase H type 2, RNase H(35), RNase H1, RNase H2, RNase H3, RNase HI, RNase HII, RNase HIII, RNaseH, Rnaseh1, RNASEH2A, RNH, RNH1, RNH2, rnhA, RnhB, rnhC, rnhD, Rv2228c, ST0519, STK_07530, Sto-RNase HI, T4 RNase H, Tk-RNase HII, Tk-Tk-RNase HII, TK0805, TRNH, TthRNH, Ty1 reverse transcriptase/RNase H, type 1 ribonuclease H, type 1 RNase H, type 2 ribonuclease H, type 2 RNase H, type II ribonuclease H

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.26 Endoribonucleases producing 5'-phosphomonoesters

                3.1.26.4 ribonuclease H

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Results	in table
16	Activating Compound
117230	AA Sequence
12	Application
1	CAS Registry Number
64	Cloned(Commentary)
35	Crystallization (Commentary)
18003	Disease/ Diagnostics
79	General Information
10	General Stability
24	IC50 Value
171	Inhibitors
10	kcat/KM [mM/s]
2	Ki Value [mM]
87	KM Value [mM]
39	Localization
233	Metals/Ions
71	Molecular Weight [Da]
69	Natural Substrates/ Products (Substrates)
441	Organism
470	PDB ID
83	pH Optimum
16	pH Range
2	pH Stability
2	pI Value
2	Posttranslational Modification
280	Protein Variants
76	Purification (Commentary)
20	Reaction
2	Reaction Type
219	Reference
6	Renatured (Commentary)
58	Source Tissue
67	Specific Activity [micromol/min/mg]
4	Storage Stability
332	Substrates and Products (Substrate)
61	Subunits
315	Synonyms
53	Temperature Optimum [°C]
6	Temperature Range [°C]
41	Temperature Stability [°C]
49	Turnover Number [1/s]

Turnover Number

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Turnover Number on EC 3.1.26.4 - ribonuclease H

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.158 - 0.4

D14R1D3:DNA18

3 entries

-

2.43

DNA-(1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid

Escherichia coli

-

pH 7.5, 21°C

678280

-

2.5

DNA-(2'-alkoxy-1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid

Escherichia coli

-

pH 7.5, 21°C

678280

-

3.97

DNA-(aza-ENA-T)-antisense-RNA hybrid

Escherichia coli

-

pH 7.5, 21°C

678280

-

2.13

DNA-(azetidine-T)-antisense-RNA hybrid

Escherichia coli

-

pH 7.5, 21°C

678280

-

1.47

DNA-(oxetane-T)-antisense-RNA hybrid

Escherichia coli

-

pH 7.5, 21°C

678280

-

1.54

DNA-RNA hybrid

Escherichia coli

-

pH 7.5, 21°C

678280

-

0.003 - 0.45

DNA-RNA-DNA hybrid

9 entries

-

0.95

DNA/RNA hybrid

Escherichia coli

-

pH 7.8-7.9, 25°C

663628

-

0.11 - 0.64

polyA*dT36

2 entries

-

0.0068 - 0.283

RNA-DNA duplex

5 entries

-

0.025 - 17

RNA-DNA heteroduplex

7 entries

-

0.133 - 0.208

RNA-DNA*DNA hybrid

4 entries

-

0.093

RNA-DNA/DNA hybrid

Pyrococcus abyssi

-

pH 8.0, 37°C, purified recombinant His-tagged enzyme

715367

-

0.0005 - 0.093

RNA-RNA duplex

2 entries

-

0.983 - 3.48

RNA18:DNA18

3 entries

-

additional information

additional information

7 entries

-

0.158

D14R1D3:DNA18

Homo sapiens

O75792

mutant R291H, pH 8.0, temperature not specified in the publication

715580

-

0.35

D14R1D3:DNA18

Homo sapiens

O75792

mutant K143I, pH 8.0, temperature not specified in the publication

715580

-

0.4

D14R1D3:DNA18

Homo sapiens

O75792

wild-type enzyme, pH 8.0, temperature not specified in the publication

715580

-

0.003

DNA-RNA-DNA hybrid

Thermus thermophilus

-

mutant D134H, pH 8.0, 30°C

654569

-

0.075

DNA-RNA-DNA hybrid

Thermus thermophilus

-

mutant A12S, pH 8.0, 30°C

654569

-

0.082

DNA-RNA-DNA hybrid

Thermus thermophilus

-

wild-type enzyme, pH 8.0, 30°C

654569

-

0.122

DNA-RNA-DNA hybrid

Thermus thermophilus

-

mutant A77P, pH 8.0, 30°C

654569

-

0.133

DNA-RNA-DNA hybrid

Thermus thermophilus

-

mutant K75M, pH 8.0, 30°C

654569

-

0.2

DNA-RNA-DNA hybrid

Thermus thermophilus

-

mutant A12S/K75M, pH 8.0, 30°C

654569

-

0.25

DNA-RNA-DNA hybrid

Thermus thermophilus

-

mutant A12S/A77P, pH 8.0, 30°C

654569

-

0.33

DNA-RNA-DNA hybrid

Thermus thermophilus

-

mutant K75M/A77P, pH 8.0, 30°C

654569

-

0.45

DNA-RNA-DNA hybrid

Thermus thermophilus

-

mutant A12S/K75M/A77P, pH 8.0, 30°C

654569

-

0.11

polyA*dT36

Methanocaldococcus jannaschii

-

pH 8.0, 500 mM NaCl, wild-type enzyme

654714

-

0.64

polyA*dT36

Methanocaldococcus jannaschii

-

pH 8.0, 50 mM NaCl, wild-type enzyme

654714

-

0.0068

RNA-DNA duplex

Pyrococcus furiosus

Q8U036

mutant F114E, 50°C, presence of Mn2+

707438

-

0.11

RNA-DNA duplex

Pyrococcus furiosus

Q8U036

mutant R113E, 50°C, presence of Mn2+

707438

-

0.188

RNA-DNA duplex

Pyrococcus furiosus

Q8U036

wild-type, 50°C, presence of Mn2+

707438

-

0.255

RNA-DNA duplex

Pyrococcus furiosus

Q8U036

mutant A115E, 50°C, presence of Mn2+

707438

-

0.283

RNA-DNA duplex

Pyrococcus furiosus

Q8U036

wild-type, 50°C, presence of Mg2+

707438

-

0.025

RNA-DNA heteroduplex

Homo sapiens

-

mutant enzyme H264A, at pH 7.5 and 30°C

729704

-

0.41

RNA-DNA heteroduplex

Pyrococcus furiosus

Q8U036

50°C, pH 8.0, divalent cation: Mn2+, mutant enzyme F114E

744264

-

3.5

RNA-DNA heteroduplex

Homo sapiens

-

wild type enzyme, at pH 7.5 and 30°C

729704

-

6.6

RNA-DNA heteroduplex

Pyrococcus furiosus

Q8U036

50°C, pH 8.0, divalent cation: Mn2+, mutant enzyme R113E

744264

-

11.3

RNA-DNA heteroduplex

Pyrococcus furiosus

Q8U036

50°C, pH 8.0, wild-type enzyme, divalent cation: Mn2+

744264

-

15.3

RNA-DNA heteroduplex

Pyrococcus furiosus

Q8U036

50°C, pH 8.0, divalent cation: Mn2+, mutant enzyme A115E

744264

-

17

RNA-DNA heteroduplex

Pyrococcus furiosus

Q8U036

50°C, pH 8.0, wild-type enzyme, divalent cation: Mg2+

744264

-

0.133

RNA-DNA*DNA hybrid

Archaeoglobus fulgidus

O29634

recombinant wild-type enzyme, pH 8.0, 30°C

656489

-

0.192

RNA-DNA*DNA hybrid

Archaeoglobus fulgidus

O29634

recombinant mutant R146A, pH 8.0, 30°C

656489

-

0.2

RNA-DNA*DNA hybrid

Archaeoglobus fulgidus

O29634

recombinant mutant R46A and K143A, pH 8.0, 30°C

656489

-

0.208

RNA-DNA*DNA hybrid

Archaeoglobus fulgidus

O29634

recombinant mutant Y164A, pH 8.0, 30°C

656489

-

0.0005

RNA-RNA duplex

Pyrococcus furiosus

Q8U036

wild-type, 50°C, presence of Mn2+

707438

-

0.093

RNA-RNA duplex

Pyrococcus furiosus

Q8U036

50°C, pH 8.0, wild-type enzyme, divalent cation: Mn2+

744264

-

0.983

RNA18:DNA18

Homo sapiens

O75792

mutant R291H, pH 8.0, temperature not specified in the publication

715580

-

2.1

RNA18:DNA18

Homo sapiens

O75792

mutant K143I, pH 8.0, temperature not specified in the publication

715580

-

3.48

RNA18:DNA18

Homo sapiens

O75792

wild-type enzyme, pH 8.0, temperature not specified in the publication

715580

-

additional information

additional information

Thermus thermophilus

-

-

134403

-

additional information

additional information

Escherichia coli

-

-

134403

-

additional information

additional information

Escherichia coli

-

-

655784

-

additional information

additional information

Homo sapiens

-

-

655784

-

additional information

additional information

Escherichia coli

-

the logarithm of the turnover number of the enzyme increases steeply with pH until a pH-independent region is reached close to neutrality

681431

-

additional information

additional information

Archaeoglobus fulgidus

O29912

kinetic parameters for the enzyme in the absence and presence of proliferating cell nuclear antigen (PCNA)

723290

-

additional information

additional information

Archaeoglobus fulgidus

-

kinetic parameters for the enzyme in the absence and presence of proliferating cell nuclear antigen (PCNA)

723290

-